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© 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Chromatography is a widely used analytical tool for separating a mixture of compounds into individual component. High performance liquid chromatography (HPLC) is one of the most important methods used for the separation, identification and quantification of a compounds present in a mixture. It meets many criteria of analysis but its main drawbacks are it is relatively time consuming to run a chromatogram and consumes high amount of solvent compared to other analytical methods. There is a need to develop a method which can overcome these drawbacks of HPLC. Ultra performance liquid chromatography (UPLC) is the new approach which opens novel direction in the field of liquid chromatography. It works on similar principle but shows better performance than conventional HPLC. UPLC is a technique of liquid chromatography with improved runtime and sensitivity with less than 2 μm particle size. The UPLC separation process is carried out under very high pressure (up to100 MPa). Additionally, it reduces the cost of reagent with shorter run time as compared to conventional HPLC. This article updated until 2020, provides a general review on the principle, instrumentation and application of UPLC in different fields of science.

Details

Title
A Mini-review on Ultra Performance Liquid Chromatography
Author
Basharat, Rabia; Kotra, Vijay; Lean Yen Loong; Mathews, Allan; Kanakal, Mahibub Mahamadsa; CH B Praveena Devi; Shaik Nyamathulla; Varala, Ravi; Long Chiau Ming; KRS Sambasiva Rao; Babu, B Hari; M Mujahid Alam
Pages
847-857
Publication year
2021
Publication date
2021
Publisher
Oriental Scientific Publishing Company
ISSN
0970020X
e-ISSN
22315039
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2807982948
Copyright
© 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.