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© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

In recent years, magnesium hydroxide has been widely studied due to its bioactivity and biocompatibility. The bactericidal effects of magnesium hydroxide nanoparticles on oral bacteria have also been reported. Therefore, in this study, we investigated the biological effects of magnesium hydroxide nanoparticles on inflammatory responses induced by periodontopathic bacteria. Macrophage-like cells, namely J774.1 cells, were treated with LPS derived from Aggregatibacter actinomycetemcomitans and two different sizes of magnesium hydroxide nanoparticles (NM80/NM300) to evaluate their effects on the inflammatory response. Statistical analysis was performed using an unresponsive Student’s t-test or one-way ANOVA followed by Tukey’s post hoc test. NM80 and NM300 inhibited the expression and secretion of IL-1β induced by LPS. Furthermore, IL-1β inhibition by NM80 was dependent on the downregulation of PI3K/Akt-mediated NF-κB activation and the phosphorylation of MAPK molecules such as JNK, ERK1/2, and p38 MAPK. By contrast, only the deactivation of the ERK1/2-mediated signaling cascade is involved in IL-1β suppression by NM300. Although the molecular mechanism involved varied with size, these results suggest that magnesium hydroxide nanoparticles have an anti-inflammatory effect against the etiologic factors of periodontopathic bacteria. These properties of magnesium hydroxide nanoparticles can be applied to dental materials.

Details

Title
Mechanisms Underlying the Suppression of IL-1β Expression by Magnesium Hydroxide Nanoparticles
Author
Koga, Ayaka 1 ; Thongsiri, Chuencheewit 2 ; Kudo, Daisuke 3 ; Dao Nguyen Duy Phuong 3 ; Iwamoto, Yoshihito 3 ; Fujii, Wataru 4   VIAFID ORCID Logo  ; Nagai-Yoshioka, Yoshie 5   VIAFID ORCID Logo  ; Yamasaki, Ryota 5   VIAFID ORCID Logo  ; Ariyoshi, Wataru 5   VIAFID ORCID Logo 

 Department of Health Sciences, Kyushu Dental University, Kitakyushu 803-8580, Fukuoka, Japan; [email protected]; Division of Infections and Molecular Biology, Department of Health Promotion, Kyushu Dental University, Kitakyushu 803-8580, Fukuoka, Japan; [email protected] (Y.N.-Y.); [email protected] (R.Y.) 
 Department of Conservative Dentistry and Prosthodontics, Srinakharinwirot University, Bangkok 10110, Thailand; [email protected] 
 SETOLAS Holdings Inc., Sakaide 762-0012, Kagawa, Japan; [email protected] (D.K.); [email protected] (D.N.D.P.); [email protected] (Y.I.) 
 Unit of Interdisciplinary Promotion, School of Oral Health Sciences, Faculty of Dentistry, Kyushu Dental University, Kitakyushu 803-8580, Fukuoka, Japan; [email protected] 
 Division of Infections and Molecular Biology, Department of Health Promotion, Kyushu Dental University, Kitakyushu 803-8580, Fukuoka, Japan; [email protected] (Y.N.-Y.); [email protected] (R.Y.) 
First page
1291
Publication year
2023
Publication date
2023
Publisher
MDPI AG
e-ISSN
22279059
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2819373165
Copyright
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.