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© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Calcium (Ca2+) flux acts as a central signaling pathway in B cells, and its alterations are associated with autoimmune dysregulation and B-cell malignancies. We standardized a flow-cytometry-based method using various stimuli to investigate the Ca2+ flux characteristics of circulating human B lymphocytes from healthy individuals. We found that different activating agents trigger distinct Ca2+ flux responses and that B-cell subsets show specific developmental-stage dependent Ca2+ flux response patterns. Naive B cells responded with a more substantial Ca2+ flux to B cell receptor (BCR) stimulation than memory B cells. Non-switched memory cells responded to anti-IgD stimulation with a naive-like Ca2+ flux pattern, whereas their anti-IgM response was memory-like. Peripheral antibody-secreting cells retained their IgG responsivity but showed reduced Ca2+ responses upon activation, indicating their loss of dependence on Ca2+ signaling. Ca2+ flux is a relevant functional test for B cells, and its alterations could provide insight into pathological B-cell activation development.

Details

Title
An Optimized Flow Cytometric Method to Demonstrate the Differentiation Stage-Dependent Ca2+ Flux Responses of Peripheral Human B Cells
Author
Bajnok, Anna 1   VIAFID ORCID Logo  ; Serény-Litvai, Timea 2   VIAFID ORCID Logo  ; Temesfői, Viktória 3   VIAFID ORCID Logo  ; Nörenberg, Jasper 4   VIAFID ORCID Logo  ; Herczeg, Róbert 5 ; Ambrus Kaposi 6 ; Berki, Timea 7 ; Mezosi, Emese 8   VIAFID ORCID Logo 

 Department of Obstetrics and Gynecology, Clinical Center, Medical School, University of Pécs, 7624 Pécs, Hungary; [email protected] (A.B.); [email protected] (J.N.); National Laboratory on Human Reproduction, University of Pécs, 7624 Pécs, Hungary; Szentágothai Research Centre, University of Pécs, 7624 Pécs, Hungary 
 National Laboratory on Human Reproduction, University of Pécs, 7624 Pécs, Hungary; Szentágothai Research Centre, University of Pécs, 7624 Pécs, Hungary; Department of Immunology and Biotechnology, Clinical Center, Medical School, University of Pécs, 7624 Pécs, Hungary 
 National Laboratory on Human Reproduction, University of Pécs, 7624 Pécs, Hungary; Szentágothai Research Centre, University of Pécs, 7624 Pécs, Hungary; Department of Laboratory Medicine, Clinical Center, Medical School, University of Pécs, 7624 Pécs, Hungary 
 Department of Obstetrics and Gynecology, Clinical Center, Medical School, University of Pécs, 7624 Pécs, Hungary; [email protected] (A.B.); [email protected] (J.N.); National Laboratory on Human Reproduction, University of Pécs, 7624 Pécs, Hungary; Department of Medical Microbiology and Immunology, Clinical Center, Medical School, University of Pécs, 7624 Pécs, Hungary 
 National Laboratory on Human Reproduction, University of Pécs, 7624 Pécs, Hungary; Szentágothai Research Centre, University of Pécs, 7624 Pécs, Hungary; Bioinformatics Research Group, Genomics and Bioinformatics Core Facility, University of Pécs, 7624 Pécs, Hungary 
 National Laboratory on Human Reproduction, University of Pécs, 7624 Pécs, Hungary; Department of Programming Languages and Compilers, Faculty of Informatics, Eötvös Loránd University, 1053 Budapest, Hungary 
 National Laboratory on Human Reproduction, University of Pécs, 7624 Pécs, Hungary; Department of Immunology and Biotechnology, Clinical Center, Medical School, University of Pécs, 7624 Pécs, Hungary 
 National Laboratory on Human Reproduction, University of Pécs, 7624 Pécs, Hungary; First Department of Internal Medicine, Clinical Center, Medical School, University of Pécs, 7624 Pécs, Hungary 
First page
9107
Publication year
2023
Publication date
2023
Publisher
MDPI AG
ISSN
16616596
e-ISSN
14220067
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2819458031
Copyright
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.