Antibacterial efficacy of greenly synthesized silver nanoparticles using nanocurcumin and silver nanoparticle gel on bovine mastitis

Abstract

This study summarizes the synthesis of AgNPs using nanocurcumin as a reducing and stabilizing agent (CurAgNPs). The stability of CurAgNPs after 12 months of storage and their antimicrobial activity against four bacteria causing mastitis in cows were investigated. Ultraviolet—visible (UV–vis) spectroscopy of the dark brownish-red stabilized CurAgNPs solution decating the surface plasmon resonance peak of the sample was observed at 438 nm. Images of the spherical CurAgNPs were obtained using transmission electron microscopy (TEM), which showed a mean particle size distribution of 15–58 nm, with a mean size of 32 nm. The influence of CurAgNPs on four microorganisms that cause mastitis in cows, Streptococcus agalactiae (S. agalactiae), Staphylococcus aureus (S. aureus), Pseudomonas aeruginosa (P. aeruginosa), and Eschericia coli (E. coli), was studied, and the minimum inhibitory concentration (MIC) was from 1.6 to 6.25 ppm. From this, the MBC of CurAgNPs were observed at 3.15 ppm for S. agalactiae, and 6.25 ppm for S. aureus, P. aeruginosa and E. coli, respectively. The formulated homogeneous gel containing 100 ppm CurAgNPs, 1.5% carboxymethyl cellulose (CMC), and distilled water was developed with a viscosity of 141 ± 7.55 cP, pH of 6.72 ± 0.11, and homogenized. The zeta potential of CurAgNPs gel after 6 months of storage is almost constant. In addition, the improved CurAgNPs gel demonstrated significant antimicrobial activity compared with tetracycline at a concentration of 100 ppm.