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© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Lignocellulosic biomass needs attention as an alternative energy source to overcome the adverse impacts of fossil fuels. Diversified Galiyat forests of Lower Himalaya may represent the potential source of lignocellulose degrading microbiota, particularly the lytic bacteria. Therefore, soil and wood samples were collected from different sites of Nathiagali and Thandiani of Galiyat forests. The soil samples collected were clayey, with a pH between 6.7 and 7.0, and with an organic matter of 2.8%–2.9% in Nathiagali and 2.1%–2.2% in Thandiani. The soils were enriched with more diversified cultivable bacteria (9 Log CFU/g) than the respective wood samples (7.4–8.6 Log CFU/g). Out of 90 bacteria, 22 isolates were efficient for cellulose degradation, 14 for xylanase activity, and 10 for laccase production. Cluster analysis showed that lignocellulolytic bacteria were grouped based on the sample medium (soil–wood) rather than the sampling site (Thandiani–Nathiagali). Efficient bacteria were also sequenced, and we found that cellulase production was prevalent in Pseudomonas spp. while laccase activity was diverse among taxonomically varied bacteria. Moreover, Stenotrophomonas sp. TS2B1 performed the best for corncob xylose degradation. Overall, the results suggest that Galiyat forests represent diverse lignocellulolytic microbial populations which should be further evaluated for applications in lignocellulosic waste management and for potential consequent fuel production.

Details

Title
Proficient Lignocellulolytic Novel Bacterial Isolates from Diversified Galiyat Forests of Lower Himalaya
Author
Malik Owais Ullah Awan 1 ; Akhtar Iqbal 1   VIAFID ORCID Logo  ; Muhammad Imtiaz Rashid 2   VIAFID ORCID Logo  ; Irshad, Usman 1   VIAFID ORCID Logo  ; Hafeez, Farhan 1   VIAFID ORCID Logo  ; Ullah, Farid 1 ; Irshad, Muhammad 1 ; Ondrasek, Gabrijel 3   VIAFID ORCID Logo  ; Mustac, Ivan 3 ; Rashid Nazir 1   VIAFID ORCID Logo 

 Department of Environmental Sciences, COMSATS University Islamabad (CUI), Abbottabad Campus, Tobe Camp, University Road, Abbottabad 22060, Pakistan 
 Center of Excellence in Environmental Studies, King Abdulaziz University, Jeddah 21589, Saudi Arabia; [email protected] 
 Department of Soil Amelioration, Faculty of Agriculture, University of Zagreb, 10000 Zagreb, Croatia; [email protected] 
First page
1180
Publication year
2023
Publication date
2023
Publisher
MDPI AG
e-ISSN
19994907
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2829811315
Copyright
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.