Shark teeth zinc isotope values document intrapopulation foraging differences related to ontogeny and sex

Abstract

Trophic ecology and resource use are challenging to discern in migratory marine species, including sharks. However, effective management and conservation strategies depend on understanding these life history details. Here we investigate whether dental enameloid zinc isotope (δ⁶⁶Zn_en) values can be used to infer intrapopulation differences in foraging ecology by comparing δ⁶⁶Zn_en with same-tooth collagen carbon and nitrogen (δ¹³C_coll, δ¹⁵N_coll) values from critically endangered sand tiger sharks (Carcharias taurus) from Delaware Bay (USA). We document ontogeny and sex-related isotopic differences indicating distinct diet and habitat use at the time of tooth formation. Adult females have the most distinct isotopic niche, likely feeding on higher trophic level prey in a distinct habitat. This multi-proxy approach characterises an animal’s isotopic niche in greater detail than traditional isotope analysis alone and shows that δ⁶⁶Zn_en analysis can highlight intrapopulation dietary variability thereby informing conservation management and, due to good δ⁶⁶Zn_en fossil tooth preservation, palaeoecological reconstructions.

Sex- and ontogeny-related differences in diet and habitat use of endangered sand tiger sharks from Delaware Bay revealed by analysis of shark teeth zinc isotope values.