Abstract

Background

Modular tissue engineering (MTE) is a novel “bottom-up” approach that aims to mimic complex tissue microstructural features. The constructed micromodules are assembled into engineered biological tissues with repetitive functional microunits and form cellular networks. This is emerging as a promising strategy for reconstruction of biological tissue.

Results

Herein, we constructed a micromodule for MTE and developed engineered osteon-like microunits by inoculating human-derived umbilical cord mesenchymal stem cells (HUMSCs) onto nHA/PLGA microspheres with surface modification of dual growth factors (BMP2/bFGF). By evaluating the results of proliferation and osteogenic differentiation ability of HUMSCs in vitro, the optimal ratio of the dual growth factor (BMP2/bFGF) combination was derived as 5:5. In vivo assessments showed the great importance of HUMSCs for osteogneic differentiation. Ultimately, direct promotion of early osteo-differentiation manifested as upregulation of Runx-2 gene expression. The vascularization capability was evaluated by tube formation assays, demonstrating the importance of HUMSCs in the microunits for angiogenesis.

Conclusions

The modification of growth factors and HUMSCs showed ideal biocompatibility and osteogenesis combined with nHA/PLGA scaffolds. The micromodules constructed in the current study provide an efficient stem cell therapy strategy for bone defect repair.

Details

Title
Dual growth factor-modified microspheres nesting human-derived umbilical cord mesenchymal stem cells for bone regeneration
Author
Song, Wenzhi; Zhao, Lanlan; Gao, Yuqi; Han, Chunyu; Gao, Shengrui; Guo, Min; Bai, Jianfei; Wang, Liqiang; Yin, Wanzhong; Wu, Feng; Zhang, Peibiao
Pages
1-20
Section
Research
Publication year
2023
Publication date
2023
Publisher
BioMed Central
e-ISSN
17541611
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2838783091
Copyright
© 2023. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.