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Abstract
Cutaneous leishmaniasis cases have increased dramatically in recent years in Nepal. The study offers molecular identification of the Leishmania species using 40 patient’s aspiration biopsy samples, targeting markers kinetoplast minicircle DNA (kDNA) and internal transcribed spacer-1 (ITS1). Among molecularly diagnosed 22 cutaneous leishmaniasis cases, L. donovani complex was identified in 13 instances and L. major in 9 cases. The ITS1 PCR was positive in 12 of the positive nested- kDNA PCR cases (12/22), confirming L. donovani complex in seven of the cases and L. major in five of the cases. In addition, the study conclude that concurrent occurrence of atypical cutaneous infections caused by L. donovani parasite in 59.1% of cases and typical cutaneous infections caused by L. major parasite in 40.9% of cases. A Phylogentic analaysis showed that the detected L. donovani species present null genetic distances from seven references of L. donovani, but slight differences between ITS1 sequences and not grouped into a significant monophyletic cluster.
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Details
1 Tribhuvan University, Central Department of Biotechnology, Kirtipur, Kathmandu, Nepal (GRID:grid.80817.36) (ISNI:0000 0001 2114 6728)
2 Sukraraj Tropical and Infectious Disease Hospital, Kathmandu, Nepal (GRID:grid.508276.e)
3 Bir Hospital, Kathmandu, Nepal (GRID:grid.414507.3) (ISNI:0000 0004 0468 8519)
4 Nepalganj Medical College and Teaching Hospital, Nepalganj, Nepal (GRID:grid.414507.3)
5 Tribhuvan University, Central Department of Zoology, Kirtipur, Kathmandu, Nepal (GRID:grid.80817.36) (ISNI:0000 0001 2114 6728)
6 Central University of Punjab, Department of Biochemistry, Bathinda, India (GRID:grid.428366.d) (ISNI:0000 0004 1773 9952)
7 McGill University, Department of Microbiology and Immunology, Montreal, Canada (GRID:grid.14709.3b) (ISNI:0000 0004 1936 8649)
8 University Montpellier, IRD, CIRAD, INTERTRYP, Montpellier, France (GRID:grid.121334.6) (ISNI:0000 0001 2097 0141)