Abstract

Acoustic-resolution photoacoustic microscopy (AR-PAM) enables visualization of biological tissues at depths of several millimeters with superior optical absorption contrast. However, the lateral resolution and sensitivity of AR-PAM are generally lower than those of optical-resolution PAM (OR-PAM) owing to the intrinsic physical acoustic focusing mechanism. Here, we demonstrate a computational strategy with two generative adversarial networks (GANs) to perform semi/unsupervised reconstruction with high resolution and sensitivity in AR-PAM by maintaining its imaging capability at enhanced depths. The b-scan PAM images were prepared as paired (for semi-supervised conditional GAN) and unpaired (for unsupervised CycleGAN) groups for label-free reconstructed AR-PAM b-scan image generation and training. The semi/unsupervised GANs successfully improved resolution and sensitivity in a phantom and in vivo mouse ear test with ground truth. We also confirmed that GANs could enhance resolution and sensitivity of deep tissues without the ground truth.

Details

Title
Enhanced resolution and sensitivity acoustic-resolution photoacoustic microscopy with semi/unsupervised GANs
Author
Le, Thanh Dat 1 ; Min, Jung-Joon 2 ; Lee, Changho 3 

 Chonnam National University, Department of Artificial Intelligence Convergence, Gwangju, Korea (GRID:grid.14005.30) (ISNI:0000 0001 0356 9399) 
 Chonnam National University Medical School and Hwasun Hospital, Department of Nuclear Medicine, Hwasun-gun, Korea (GRID:grid.14005.30) (ISNI:0000 0001 0356 9399) 
 Chonnam National University, Department of Artificial Intelligence Convergence, Gwangju, Korea (GRID:grid.14005.30) (ISNI:0000 0001 0356 9399); Chonnam National University Medical School and Hwasun Hospital, Department of Nuclear Medicine, Hwasun-gun, Korea (GRID:grid.14005.30) (ISNI:0000 0001 0356 9399) 
Pages
13423
Publication year
2023
Publication date
2023
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2852213576
Copyright
© The Author(s) 2023. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.