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© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

The stingless bee Partamona helleri plays a role in pollinating both native and cultivated plants in the Neotropics. However, its populations can be reduced by the pyrethroid insecticide lambda-cyhalothrin. This compound may cross the intestinal barrier and circulate through the hemolymph, affecting various non-target bee organs. The aim of the present study was to assess the extent of cellular damage in the midgut and the resulting oxidative stress caused by lambda-cyhalothrin in P. helleri workers. Bees were orally exposed to lambda-cyhalothrin. The lethal concentration at which 50% of the bees died (LC50) was 0.043 mg a.i. L−1. The P. helleri workers were fed this concentration of lambda-cyhalothrin and their midguts were evaluated. The results revealed signs of damage in the midgut epithelium, including pyknotic nuclei, cytoplasm vacuolization, changes in the striated border, and the release of cell fragments, indicating that the midgut was compromised. Furthermore, the ingestion of lambda-cyhalothrin led to an increase in the activity of the detoxification enzyme superoxide dismutase and the levels of the NO2/NO3 markers, indicating oxidative stress. Conversely, the activities of the catalase and glutathione S-transferase enzymes decreased, supporting the occurrence of oxidative stress. In conclusion, the ingestion of lambda-cyhalothrin by P. helleri workers resulted in damage to their midguts and induced oxidative stress.

Details

Title
Midgut Cell Damage and Oxidative Stress in Partamona helleri (Hymenoptera: Apidae) Workers Caused by the Insecticide Lambda-Cyhalothrin
Author
João Victor de Oliveira Motta 1   VIAFID ORCID Logo  ; Lenise Silva Carneiro 1 ; Martínez, Luís Carlos 2   VIAFID ORCID Logo  ; Daniel Silva Sena Bastos 1   VIAFID ORCID Logo  ; Matheus Tudor Candido Santos Resende 1 ; Bárbara Monteiro Castro Castro 3 ; Mariana Machado Neves 1   VIAFID ORCID Logo  ; José Cola Zanuncio 3   VIAFID ORCID Logo  ; Serrão, José Eduardo 1   VIAFID ORCID Logo 

 Department of General Biology, Institute of Biotechnology Applied to Agriculture, Federal University of Viçosa, Viçosa 36570-900, Brazil; [email protected] (J.V.d.O.M.); [email protected] (L.S.C.); [email protected] (D.S.S.B.); [email protected] (M.T.C.S.R.); [email protected] (M.M.N.) 
 Faculdad de Ciencias Agricolas, Universidad de Narino, Pasto 522020, Colombia; [email protected] 
 Department of Entomology, Institute of Biotechnology Applied to Agriculture, Federal University of Viçosa, Viçosa 36570-900, Brazil; [email protected] (B.M.C.C.); [email protected] (J.C.Z.) 
First page
1510
Publication year
2023
Publication date
2023
Publisher
MDPI AG
e-ISSN
20763921
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2856765244
Copyright
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.