Special Section Guest Editorial: Fluorescence Lifetime Imaging, Optical Micromechanics, and Beyond

Abstract

The editorial introduces the Journal of Biomedical Optics Special Section on Selected Topics in Biophotonics: Fluorescence Lifetime Imaging and Optical Micromechanics.

The present special section of the Journal of Biomedical Optics Volume 25 Issue 7, entitled “Fluorescence Lifetime Imaging and Optical Micromechanics,” comprises two invited tutorial papers and several contributed papers from the summer school Biophotonics ’19¹ as well as contributed papers within the general scope of the school. This section is meant to reflect highlights from the summer school, and serves as an opportunity for participants to publish the work discussed during the school in a collected fashion for everyone to read. After now completing the Biophotonics ’19 summer school with this special section, we are very much looking forward to celebrating the anniversary of the school at Biophotonics ’21 (this school being the 10^th in the series¹) and the accompanying JBO special section.

Motivation and Purpose of Biophotonics Graduate Schools

Over the past decade, lasers, optical methods, and instruments based on light interaction with tissues have emerged as powerful techniques for medical diagnostics, monitoring wide spectra of tissue function and pathology. In biophysics and biology, optical sensing and manipulation of cells have strengthened understanding of basic cell function. Together with improved laser therapeutic techniques, optical sensing and cell manipulation form the basis for increasing interest in biophotonics. Throughout Europe, the USA, and the rest of the world, major research centers are highly active in this field that in a broad sense may be labeled biophotonics. Therefore, education within this area is increasingly important.

The main purpose with the biennial graduate summer school is to provide education within biophotonics for students and young scientists at the highest international level. Our aim is to attract internationally renowned researchers as lecturers who would attract the most talented young researchers worldwide in the field of biophotonics.

Format of the Biophotonics Graduate Summer School

The school mainly targets graduate students and postdoctoral fellows from around the world. The format of the school is a combination of lectures and student poster presentations, with time between lectures for discussions and exchange of scientific ideas. The lecturers cover one topic in a full session comprising four lectures, which thoroughly covers the basics and state-of-the-art of each topic. On one hand, this choice limits the number of topics taught at each school. On the other hand, the topics selected for the schools are covered in detail. Therefore, the range of topics taught will change from school to school.

An important feature of the school format is that students and lecturers spend the entire week together, which provides excellent opportunities for the exchange of scientific ideas, networking, and socializing.

The 9^th International Graduate Summer School Biophotonics ’19¹ covered the basics of lasers as well as supercontinuum sources and their application in medicine, tissue optics, photodynamic therapy, optical manipulation and optical tweezers, and their applications in biophotonics, optical micromechanics and speckle (covered in one invited tutorial paper), diffuse optical and molecular imaging, optical coherence tomography, photo-acoustic tomography, fluorescence life-time imaging (covered in another invited tutorial paper), and coherent Raman scattering microscopy. In addition, at the kick-off of the school, entrepreneurship was covered in two keynote lectures. One of these two lectures was given by Dr. Cuccia (Modulim, USA), an alumni of the very first school, Biophotonics 2003, who spoke on his journey from PhD student, to inventor, to CEO of a start-up company selling FDA-approved medical devices based on his own original ideas. Prof. Sune Svanberg (Lund University, Sweden) delivered a third keynote on spectroscopy in life sciences.

Special Section in the Journal of Biomedical Optics

We are pleased to introduce the contributions to this special section on “Fluorescence Lifetime Imaging and Optical Micromechanics” comprising two invited tutorial papers and four contributed papers, from participants of the school and from other researchers in the field. Not all of the school-participant contributions fall within the themes covered by the special section, but all contributions reflect the core topics of the school and span the fields of biomedical optics and biophotonics. The invited tutorial papers are:

• Rupsa Datta et al., “Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications,” J. Biomed. Opt. 25, 071203 (13 May 2020), doi 10.1117/1.JBO.25.7.071203

• Zeinab Hajjarian and Seemantini K. Nadkarni, “Tutorial on laser speckle rheology: technology, applications, and opportunities,” J. Biomed. Opt. 25, 050801 (1 May 2020), doi 10.1117/1.JBO.25.5.050801.

The first paper, from a lecturer (Prof. Melissa Skala) at the school, provides an excellent background to the field of fluorescence lifetime imaging microscopy, whilst also reviewing instrumentation and applications. The second invited tutorial, also from a lecturer (Prof. Seemantini Nadkarni) at the school, covers speckle-based measurement techniques and their applications for imaging cell micromechanics. These tutorials further build the online JBO resources for high quality teaching material and provide a natural continuation to previous tutorial papers on the foundation of diffuse optics,² imaging thick tissues with diffuse optics,³ molecular imaging,⁴ optical micromanipulation,⁵ photodynamic therapy,⁶ optical coherence tomography,⁷ biological imaging with coherent Raman scattering microscopy,⁸ in vivo applications of Raman spectroscopy,⁹ photoacoustic tomography,¹⁰ and fiber-based sources for biophotonics¹¹ published in similar special sections from previous schools. These papers all belong to a planned series of tutorial review papers from each biennial school that provide high-level, open-access educational material for the benefit of the scientific community and, in addition, fulfill our own motivation for creating the school in the first place. With this special section, there are now in total 12 excellent tutorials that provide profound introduction into the basics of our field.

In addition to the invited tutorial papers, we have four contributed papers. Erkkilä et al. https://doi.org/10.1117/1.JBO.25.7.071202 demonstrate a surgical microscope platform with fluorescence lifetime imaging for neurosurgery. They demonstrate the capabilities in guiding of surgery for 5-ALA-labeled glioblastoma tissue. , James et al. https://doi.org/10.1117/1.JBO.25.7.071204 demonstrate fluorescence lifetime imaging in a two-photon microscope, i.e., combining tomography and lifetime measurements, investigating sentinel lymph nodes. Calcium-induced epidermal differences are investigated in vitro by using two-photon microscopy to better understand its diagnostic potential (label-free biomarker) by , Malak et al. https://doi.org/10.1117/1.JBO.25.7.071205 Finally, and Meyer et al. https://doi.org/10.1117/1.JBO.25.7.071206 present a method for optimal selection of emission spectral bands in multiphoton microscopy of biomarkers. The technique is demonstrated using autofluorescence spectra of 2D and 3D cell cultures.

Acknowledgments

We would first like to thank all the lecturers and participants of the summer school Biophotonics ’19 for making the school a very fruitful and positive experience, the authors of all papers in this special section for their excellent contributions, and the many reviewers around the world who provided high-quality reviews of the manuscripts. Your dedicated efforts have made this high-quality special section possible. We would also like to thank the JBO publication staff, Renae Keep and Rita Davis in particular, for their invaluable support and prompt assistance in helping meet the JBO standards. Finally, the editors would like to thank Prof. Brian Pogue, Editor-in-Chief, for his strong support of the Biophotonics school, as well as suggestions and stimulating encouragements during the entire process of developing this special section.

Stefan Andersson-Engels is head of Biophotonics at IPIC (Irish Photonic Integration Ctr.). He graduated in engineering physics from Lund University in 1985, and received his PhD in physics from Lund University in 1990. He was then a postdoc at McMaster University in Ontario, Canada, from 1990 to 1991. After a few years as lecturer he was appointed professor in 1999 at Lund University. He has published extensively in the area of biomedical optics, with 200 papers in peer-reviewed journals and an h-index of 60. His main interest is optical spectroscopy and photodynamic therapy, while he also is active in improving the understanding in fundamental aspects of light propagation in turbid media. He is a senior advisory editor for the Journal of Biomedical Optics.

Peter E. Andersen received his MSc degree in electrical engineering in 1991 and his PhD from the Technical University of Denmark in 1995. He is with DTU Health Tech at the Technical University of Denmark and group leader of the Biophotonic Imaging Group. He has more than 20 years of research experience within biomedical optics, light source technology, optical coherence tomography systems, nonlinear microscopy, and translation of these modalities into clinical applications, including endoscopic applications. Currently, he is an editorial board member of the Journal of Biomedical Optics.

References

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