Abstract

To analyze protective/regenerative effects of adipose tissue-derived mesenchymal stem cells (ADMSC) on 131I-Radioiodine (RAI)-induced salivary gland damage in rats.

Study population consisted of controls (n:6) and study groups (n:54): RAI (Group 1), ADMSC (Group 2), amifostine (Group 3), RAI+amifostine (Group 4), concomitant RAI+ADMSC (Group 5) and RAI+ADMSC after 48 h (Group 6). We used light microscopy (LM), transmission electron microscopy (TEM), and salivary gland scintigraphy (SGS), and analyzed data statistically.

We observed the homing of ADMSC in salivary glands at 1st month on LM. RAI exposure affected necrosis, periductal fibrosis, periductal sclerosis, vascular sclerosis and the total sum score were in a statistically significant manner (P < 0.05). Intragroup comparisons with LM at 1st and 6th months revealed statistically significant improvements in Group 6 (P < 0.05) but not in Groups 4 and 5. Intergroup comparisons of the total score showed that Groups 4 and 5 in 1st month and Group 6 in 6th month had the lowest values. TEM showed vacuolization, edema, and fibrosis at 1st month, and an improvement in damage in 6th month in Groups 5 and 6. SGSs revealed significant differences for the maximum secretion ratio (Smax) (P = 0.01) and the gland-to-background ratio at a maximum count (G/BGmax) (P = 0. 01) at 1st month, for G/BGmax (P = 0.01), Smax (P = 0.01) and the time to reach the maximum count ratio over the time to reach the minimum count (Tmax/Tmin) (P = 0.03) at 6th month. 1st and 6th month scans showed differences for Smax and G/BGmax (P = 0.04), but not for Tmax/Tmin (p > 0.05). We observed a significant deterioration in gland function in group 1, whereas, mild to moderate deteriorations were seen in protective treatment groups.

Our results indicated that ADMSC might play a promising role as a protective/regenerative agent against RAI-induced salivary gland dysfunction.

Details

Title
Protective/restorative role of the adipose tissue-derived mesenchymal stem cells on the radioiodine-induced salivary gland damage in rats
Author
Saylam, Güleser 1 ; Bayır, Ömer 1 ; Gültekin, Salih Sinan 2 ; Ferda Alparslan Pınarlı 3 ; Han, Ünsal 4 ; Korkmaz, Mehmet Hakan 5 ; Mehmet Eser Sancaktar 6 ; Tatar, İlkan 7 ; Sargon, Mustafa Fevzi 7 ; Tatar, Emel Çadallı 1 

 University of Health Sciences, Dışkapı Yıldırım Beyazıt Training and Research Hospital, Department of Otolaryngology, Head and Neck Surgery, Ankara, Turkey 
 University of Health Sciences, Dışkapı Yıldırım Beyazıt Training and Research Hospital, Department of Nuclear Medicine, Ankara, Turkey 
 University of Health Sciences, Dışkapı Yıldırım Beyazıt Training and Research Hospital, Department of Stem Cell and Genetic Diagnostic Center, Ankara, Turkey 
 University of Health Sciences, Dışkapı Yıldırım Beyazıt Training and Research Hospital, Department of Pathology, Ankara, Turkey 
 Yıldırım Beyazıt University, Faculty of Medicine, Department of Otolaryngology, Head and Neck Surgery, Ankara, Turkey 
 Ministry of Health, Samsun Training and Reseach Hospital, Department of Otolaryngology, Head and Neck Surgery, Samsun, Turkey 
 Hacettepe University, Faculty of Medicine, Department of Anatomy, Ankara, Turkey 
Pages
307-316
Publication year
2017
Publication date
2017
Publisher
De Gruyter Poland
ISSN
13182099
e-ISSN
0485893X
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2860546527
Copyright
© 2017. This work is published under http://creativecommons.org/licenses/by/4.0 (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.