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© 2021. This work is licensed under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Significance: Gene expression analysis is an important fundamental area of biomedical research. However, live gene expression imaging has proven challenging due to constraints in conventional optical devices and fluorescent reporters.

Aim: Our aim is to develop smaller, more cost-effective, and versatile imaging capabilities compared with conventional devices. Bioluminescence reporter-based gene expression analysis was targeted due to its advantages over fluorescence-based imaging.

Approach: We created a small compact imaging system using micro-CMOS image sensors (μCIS). The μCIS model had an improved pixel design and a patterned absorption filter array to detect the low light intensity of bioluminescence.

Results: The device demonstrated lower dark current, lower temporal noise, and higher sensitivity compared with previous designs. The filter array enabled us to subtract dark current drift and attain a clearer light signal. These improvements allowed us to measure bioluminescence reporter-based gene expression in living mammalian cells.

Conclusion: Using our μCIS system for bioluminescence imaging in the future, the device can be implanted in vivo for simultaneous gene expression imaging, behavioral analysis, and optogenetic modulation.

Details

Title
Ultrasmall compact CMOS imaging system for bioluminescence reporter-based live gene expression analysis
Author
Olorocisimo, Joshua Philippe; Briones, Jeric; Sasagawa, Kiyotaka; Haruta, Makito; Takehara, Hironari; Tashiro, Hiroyuki; Ishida-Kitagawa, Norihiro; Bessho, Yasumasa; Ohta, Jun
First page
116002
Section
Imaging
Publication year
2021
Publication date
Nov 2021
Publisher
S P I E - International Society for
ISSN
1083-3668
e-ISSN
1560-2281
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2862327151
Copyright
© 2021. This work is licensed under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.