Abstract

Proper disinfection and inactivation of highly pathogenic viruses is an essential component of public health and prevention. Depending on environment, surfaces, and type of contaminant, various methods of disinfection must be both efficient and available. To test both established and novel chemical disinfectants against risk group 4 viruses in our maximum containment facility, we developed a standardized protocol and assessed the chemical inactivation of the two Ebola virus variants Mayinga and Makona suspended in two different biological soil loads. Standard chemical disinfectants ethanol and sodium hypochlorite completely inactivate both Ebola variants after 30 s in suspension at 70% and 0.5% v/v, respectively, concentrations recommended for disinfection by the World Health Organization. Additionally, peracetic acid is also inactivating at 0.2% v/v under the same conditions. Continued vigilance and optimization of current disinfection protocols is extremely important due to the continuous presence of Ebola virus on the African continent and increased zoonotic spillover of novel viral pathogens. Furthermore, to facilitate general pandemic preparedness, the establishment and sharing of standardized protocols is very important as it allows for rapid testing and evaluation of novel pathogens and chemical disinfectants.

Details

Title
Virucidal activity of three standard chemical disinfectants against Ebola virus suspended in tripartite soil and whole blood
Author
Jonsdottir, Hulda R. 1 ; Zysset, Daniel 2 ; Lenz, Nicole 3 ; Siegrist, Denise 2 ; Ruedin, Yelena 2 ; Ryter, Sarah 4 ; Züst, Roland 2 ; Geissmann, Yannick 2 ; Ackermann-Gäumann, Rahel 5 ; Engler, Olivier B. 2 ; Weber, Benjamin 2 

 Federal Office for Civil Protection, Spiez Laboratory, Spiez, Switzerland (GRID:grid.482328.7) (ISNI:0000 0004 0516 7352); Inselspital University Hospital, Department of Rheumatology, Immunology, and Allergology, Bern, Switzerland (GRID:grid.411656.1) (ISNI:0000 0004 0479 0855); University of Bern, Department of BioMedical Research, Bern, Switzerland (GRID:grid.5734.5) (ISNI:0000 0001 0726 5157) 
 Federal Office for Civil Protection, Spiez Laboratory, Spiez, Switzerland (GRID:grid.482328.7) (ISNI:0000 0004 0516 7352) 
 Federal Office for Civil Protection, Spiez Laboratory, Spiez, Switzerland (GRID:grid.482328.7) (ISNI:0000 0004 0516 7352); University of Lausanne, Institute of Microbiology, Lausanne University Hospital, Lausanne, Switzerland (GRID:grid.9851.5) (ISNI:0000 0001 2165 4204); Federal Office for Agriculture, Agroscope, Bern, Switzerland (GRID:grid.484687.1) (ISNI: 0000 0001 1457 2921) 
 Federal Office for Civil Protection, Spiez Laboratory, Spiez, Switzerland (GRID:grid.482328.7) (ISNI:0000 0004 0516 7352); Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany (GRID:grid.424065.1) (ISNI:0000 0001 0701 3136) 
 Federal Office for Civil Protection, Spiez Laboratory, Spiez, Switzerland (GRID:grid.482328.7) (ISNI:0000 0004 0516 7352); ADMED Microbiologie, La Chaux-de-Fonds, Switzerland (GRID:grid.482328.7) 
Pages
15718
Publication year
2023
Publication date
2023
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2866963271
Copyright
© The Author(s) 2023. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.