1. Introduction
The genus Cortinarius is the most species-rich fungal species, even after its revision and emendation by Liimatainen et al. [1]. Its subgenus Telamonia itself represents the largest currently recognized part of the genus with 184 species and 80 sections identified by Liimatainen et al. [2]. In the Mediterranean, temperate, and arctic–alpine zones of the Northern Hemisphere, Telamonia species are dominant in ectomycorrhizal communities, although their taxonomy remains one of the least resolved according to the above-mentioned molecular works. Alpine communities associated with dwarf Salix species, Dryas octopetala, and Polygonum viviparum are especially dominated by small Telamonia species.
Jules Favre [3,4] was the first meticulous observer of these Lilliputian communities in the hardly accessible slopes of the Swiss National Park, from where he reported 26 species of Cortinarius, 19 of them new to science. After Favre’s era, 25 years of intensive prospections took place, carried out by Robert Kühner and Denise Lamoure in the French alpine zone of the National Park of Vanoise [5,6]. A total of 38 species of Cortinarius were recorded by them, 10 newly described [7,8], and 1 previously described by another famous promotor of alpine mycology, Marcel Bon [9]. Finally, Bon keyed out 56 species for the European alpine zone [10], 38 of them in the currently delimited subgen. Telamonia.
With the exception of Cortinarius tatrensis Fellner & Landa described from the Tatras Mountains [11], and in spite of intensive forays into many alpine areas throughout Europe, no new Cortinarius species were subsequently published from these ecosystems, indicating that Favre’s [3,12], Lamoure’s [7,8,13], and Bon’s [10] taxonomies remained the reference works for the identification of alpine Cortinarius in Europe for more than three decades.
The DNA-based revisions of Telamonia simultaneously published by Liimatainen et al. [2] and, specifically focusing on alpine taxa, by Kokkonen [14], have clearly highlighted the need to revise the entire traditionally accepted taxonomy for these small fungi. The sequencing of the type collections was a key step for this ambitious challenge. Liimatainen et al. [2] published 11 ITS type sequences from Favre’s alpine species of Telamonia, and Kokkonen [14] published 6 more from Favre’s species and 3 from Lamoure’s taxa. While most species have thus been elucidated (unlocking the identification of many arctic–alpine collections and environmental samplings), those which could not be sequenced for various reasons remain enigmatic.
A series of prospections in the Vanoise region (Savoie, France) close to Kühner and Lamoure’s areas by F. A. and P.-A. M. between 2010 and 2019, and privileged access to D. Lamoure’s herbarium (LY) in 2020, yielded important alpine material that could be sequenced and compared to the data already published [2,14]. Most of the collections could be attributed to taxa already reported from Vanoise [6] and confirmed here, but some of them were of special interest and are the object of the present article. Introduced here are Cortinarius infidus sp. nov., a sibling species of C. inops J. Favre (sect. Verni), C. dryadophilus sp. nov. (sect. Castanei), and C. saniosopygmaeus sp. nov. (sect. Saniosi). A sequence obtained from the holotype of C. caesionigrellus Lamoure reveals the phylogenetic identity of this taxon within the sect. Flexipedes. A recent sequenced collection of this species further allows its taxonomic update and illustration for the first time. In addition, the new combinations Cortinarius flexipes var. brachyspermus (Lamoure) comb. nov., based on type revision, and Thaxterogaster dovrensis (Brandrud) comb. & stat. nov., with a description of the first record from the alpine zone, are introduced here.
2. Materials and Methods
Studied material: Recent collections were carried out in Savoie, France, by F. A., and in Lombardy, Italy by A. F., all in alpine areas. Type and additional collections are kept in the herbarium of the University of Lyon, France (LY), and exceptions are mentioned in the “Material studied” parts in the Taxonomy section.
Morphological studies: fresh basidiomata were photographed in situ and the habitat, altitude, soil characteristics, and significant plants were noted. Detailed observations of macromorphological characters were performed on fresh, dehydrated, and rehydrated material, and compared to photographic material and field notes. Micro-anatomical studies were conducted on sections from the pileus, stipe, and lamellae, revived and mounted in 10% ammoniacal Congo red, or in 3–5% KOH. Spore measurements were taken from natural deposits on the stipe surface obtained from dry basidiomata, mounted in water or in Congo red, and measured using the freeware Piximètre 5.10 [15]. A minimum of 30 spores were measured from each basidiome and the Me (average length and width), Q (minimum and maximum length/width ratio), and Qm (average length/width ratio) were calculated. The spore measurements exclude the apiculus.
DNA extraction, amplification, and sequencing: DNA extraction and PCR amplification were conducted with the REDExtract-N-Amptm Plant PCR Kit (Sigma-Aldrich, St. Louis, MO, USA), following the manufacturer’s instructions. The internal transcribed spacers and 5.8S of nuc rDNA (ITS barcode) were amplified from each collection, with the primers ITS-1F, ITS-4b, ITS-4, ITS-2, and ITS-3 [16,17], as described in [18]. The amplicons were purified by Eurofins Genomics, Ebersberg, Germany, prior to sequencing. Raw sequence data were edited and assembled with Codon Code Aligner 4.1.1 (CodonCode Corp., Centerville, MA, USA), and deposited in GenBank under the accession numbers indicated in the Taxonomy section below (type material and additional material).
Phylogenetic analyses: The ITS sequence dataset analysed here was assembled by combining 19 newly generated sequences with 54 phylogenetically close published sequences from the Cortinarius subgen. Telamonia, identified by BLAST searches in GenBank and UNITE. Multiple sequence alignment was carried out with MUSCLE 3.7 [19] and edited manually to adjust some homologous indels. Maximum likelihood phylogenetic analysis of the aligned sequences was performed online at www.phylogeny.lirmm.fr [20] with PhyML 3.0 [21], using the GTR model of evolution. Branch support was assessed using the non-parametric, Shimodaira–Hasegawa, version of the approximate likelihood-ratio test (SH-aLRT), implemented in the latest release of PhyML and which ensures high accuracy when SH-aLRT > 0.81 [22]. The phylogenetic tree (depicted in Figure 1) was edited with Inkscape 0.91 (
3. Results and Discussion
3.1. New Taxa in Cortinarius Sect. Castanei, Sect. Verni, and Sect. Saniosi
Cortinarius dryadophilus Armada, Bellanger & P.-A. Moreau, sp. nov. MycoBank MB849751. (Figure 2A,B).
Etymology: Latin, that likes Dryas thickets, relative to the ecology of this species.
Type material: FRANCE, Savoie, Peisey-Nancroix, along the GR5 path on the way to the lake of La Plagne, 2050 m asl, among Dryas octopetala, 25 August 2018, F. Armada (holotype LY FA4330). GenBank ITS OR419975.
Diagnosis: A date-brown or vinaceous-brown «Decipientes» with reddish tinges in the stipe evoking a small-spored C. subtribulosus, in Dryas thickets.
Pileus 15–28 mm in diameter, conical to conical-convex; surface vinaceous reddish-brown to dark date-brown at the centre, lighter towards the margin, hygrophanous, covered by a whitish fibrillose or araneous veil, abundant especially at the margin; margin slightly incurved, early fimbriate, whitish, appendiculated by thin remnants of cortina. Lamellae up to 4 mm wide, crowded, thin, adnate, rather irregular in length, beige to dark beige, and rusty brown when mature; edge entire, somewhat sinuous, subconcolorous to the faces or paler. Stipe 31–43 × 3–5 mm, slender, cylindrical fistulose on adults; surface distinctly blueish at the apex, below entirely covered by a whitish fibrillose-silky veil forming a small annuliform zone at the upper third, hiding a vinaceous reddish-brown background visible by detersion. Context moderately thick at the centre, thin elsewhere, and reddish-brown or vinaceous, lilacine at the stipe apex. Smell weak, pleasant, and slightly fruity or pastry-like. Taste mild. Spore print not obtained, cinnamon ochre on stipe surface. KOH reaction: bistre-brown on pileus surface and context.
Spores (7.5) 8–9.5 (10.5) × (5.25) 5.5–6 (6.5) µm, ellipsoid-pruniform, with a fairly marked hilar depression, strongly ornamented by pointed or echinulate-truncate warts up to 0.5 µm high, higher at the apex where they can reach 1 (1.25) µm. Basidia 28–46 × 8–10 µm, cylindrical to slightly clavate, tetrasporic, and clamped. Sterile marginal cells 4–8 µm wide, common, and basidioid; edge fertile. Pileipellis made of a thin layer of narrow hyphae 1–5 µm wide covering sausage-shaped hyphae up to 16 µm wide; strong yellow or dark yellow pigmentation, wall coating, sometimes finely incrusting the upper hyphae; clamped.
Habitat and distribution: So far only known from the type locality but most likely present, albeit overlooked or misidentified, in other ecologically similar areas.
Notes: We introduce C. dryadophilus as a new species here based on a single collection, because of its unique molecular signature and because its ecology may help identify it in the field. The ITS sequence of this species is unknown from the GenBank and UNITE public databases, but it clearly belongs to the sect. Castanei [2] (Figure 1). This lineage encompasses the contested neotype of C. castaneus (Bull.) Fr., that of C. decipiens (Pers.) Fr., several historical names that were sometimes merged into a C. decipiens sensu lato concept [23], and species more recently identified or validated by molecular tools, such as C. cistocastaneus Armada & Bellanger [24] and C. rufomyrrheus Eyssart., Sleiman & Bellanger [25]. As delineated in “Mission Impossible” [2], sect. Castanei consists of a strongly supported clade of mostly European species, associated with a handful of North American, more distantly related species, some yet to be named. Our new species nests in a basal position to the European clade and its inclusion in the latter clade further supports it (Figure 1). Its phylogenetic closest neighbour is C. subodoratus Bidaud, from which it differs by five nucleotide changes (three SNPs + two indels) [2].
Prior to DNA sequencing, we intended to relate C. dryadophilus to previously known alpine species [3,7,8,10]. Cortinarius basiroseus P.D. Orton, originally described from the calcicolous beech forest of Netley Park in southern England [26], was targeted because of apparently compatible colours and microscopic features. The sequencing of the holotype of C. basiroseus, successfully achieved during this work, evidenced the unexpected synonymy of this name (with nomenclatorial priority) with C. rubrocinctus Reumaux and C. uraceoarmillatus Bidaud, which together founded the sect. Rubrocincti [2] distant from sect. Castanei to which C. dryadophilus belongs.
Another red-stiped alpine Cortinarius sect. Telamonia is C. tatrensis [11]. A short sequence from the holotype of this species is available [2] and, although its phylogenetic placement remains uncertain, its conspecificity with the present species can be ruled out.
Morphologically, C. dryadophilus differs from C. castaneus (neotype) by larger spores (8–9.5 × 5.5–6 vs. 6.5–8 × 4.5–5.5 µm, respectively), lamella colour (beige or dark beige vs. bright reddish-ochre, respectively), and a different smell (fruity or pastry-like vs. slightly raphanoid to cedarwood, respectively). Another closely related species is C. decipiens, neotypified [27] by a collection from France under Picea abies, and that has since been widely observed from the plains to the Alps under various tree species. Its spores are of the same size as those of C. dryadophilus and only the colour of the lamellae, perhaps more often with violet tinges, may tell the two species apart. As a perfect lookalike of C. decipiens, C. falsosus Moën.-Locc. & Reumaux [28] displays the same risks of confusion with C. dryadophilus. With darker colours of both the pileus and lamellae, C. fuscoflexipes M.M. Moser & McKnight [29] is macroscopically more distinct from our species, but it produces similar spores. Despite being originally described from Yellowstone National Park in the USA, it was recently reported in Finland [14] and may thus co-occur in alpine localities with C. dryadophilus. The recently described C. rufomyrrheus [25] also displays real morphological similarities with C. dryadophilus but differs by smaller and narrower spores and a smell evoking burnt meat. Quite surprisingly, the species most resembling C. dryadophilus macroscopically does not belong to the sect. Castanei, but to sect. Bombycini. Cortinarius subturibulosus Kizlik & Trescol is indeed a perfect lookalike of the present species, with fortunately larger spores and usually distinctive smell of orange blossoms [30]; both may co-occur in the same Dryas patches, as was the case for our collection.
Cortinarius infidus Armada, Bellanger & P.-A. Moreau, sp. nov. MycoBank MB849752. (Figure 3A–F).
Etymology: Latin, unfaithful, referring to the misleading taxonomic features of this species, and associated confusion with its sister species C. inops.
Type material: FRANCE, Savoie, Peisey-Nancroix, Lac Marlou, 2500 m asl, among Salix herbacea, S. retusa, and Polygonum viviparum, 21 August 2018, F. Armada (LY FA4276, holotype). GenBank ITS OR420012.
Diagnosis: Similar to Cortinarius inops from which it differs by somewhat longer spores; otherwise characterized by a conspicuous white veil visible at least at the pileus margin, vinaceous-red tinges at least in the stipe flesh, spores reaching 10 µm, and a strict association with dwarf Salix carpets.
Additional material: FINLAND, Keski-Pohjanmaa, Kalajoki, Hiekkasärkät, near Salix phylicifolia and Alnus sp., sandy soil, 10 September 2004, K. Kokkonen 255/04 (not studied), GenBank ITS MN841140; Kokkola, Lohtaja, Vattajaniemi, beach, among S. repens and Pinus sylvestris seedlings, 11 September 2004, K. Kokkonen 265/04 (not studied), GenBank ITS MN841141; Pietarsaari, Fäboda, beach, near Salix phylicifolia, with Alnus, Pinus, and Betula spp. further away, 12 September 2004, K. Kokkonen 280/04 (not studied), GenBank ITS MN841143. FRANCE, Savoie, Peisey-Nancroix, Lac Marlou, 2500 m asl, among S. herbacea, 17 August 2010, F. Armada & E. Armada, FA 1734, GenBank ITS OR420007; ibid., among Salix herbacea, S. retusa, and Polygonum viviparum, 21 August 2018, F. Armada, FA 4281, GenBank ITS OR420013; ibid., Val-d’Isère, Col de l’Iseran, among S. herbacea (S. retusa, S. reticulata, S. hastata, and P. viviparum nearby), 2650 m asl, 23 August 2018, F. Armada, FA 4298, GenBank ITS OR420014. SWEDEN. Lapland, Lule Lappmark, Gällivare, Nieras, among S. herbacea, 30 August 2016, K. Kokkonen 1395/16 (not studied), GenBank ITS MN841144. SWITZERLAND, Graubünden. Scuol, Lai Sesvenna, among S. herbacea, 2660 m asl, 17 August 2017, K. Kokkonen 281/17 (not studied), GenBank ITS MN841142; ibid., 25 August 2019, K. Kokkonen 38/19 (not studied), GenBank ITS MN841150. Cortinarius inops: FRANCE, Savoie, Peisey-Nancroix, col de la Chal, 2500 m asl, among S. retusa, 25 August 2005, F. & E. Armada, FA 27, GenBank ITS OR420011; ibid., 13 August 2010, FA 1697, GenBank ITS OR420006; Val d’Isère, col de l’Iseran, 2650 m asl, among S. herbacea, S. retusa, and S. reticulata (P. viviparum and S. hastata nearby), 23 August 2018, F. Armada, FA 4310, GenBank ITS OR420015; ibid., 14 August 2019, FA 4874, GenBank ITS OR420016; Haute-Savoie, Les Contamines-Montjoie, col du Joly, 2000 m asl, among S. reticulata, S. retusa, and Dryas octopetala, 22 August 2010, F. & E. Armada, A. Bidaud & R. Fillion, FA 1767, GenBank ITS OR420008.
Pileus 4–33 mm in diameter, conical to conical-convex and remaining so, with an obtuse but pronounced umbo, sometimes acute; surface faintly viscid when wet, quickly dry, glabrous, entirely covered by a white fibrillose veil, abundant at the margin when young, on an almost black background, then reddish-brown to brownish, strongly hygrophanous, drying from the centre to reddish-brown, vinaceous-brown, or dark chestnut; margin incurved, sometimes crenulate or striate, incised on very old specimens, becoming fibrillose-fissurate at the end (like Inocybe spp.). Lamellae up to 5–6 mm wide, rather thick, broadly adnate, moderately crowded, light brown café-au-lait or beige, then early brown to rusty-brown; edges smooth, concolorous, or slightly paler than faces. Stipe 10–45 × 1.5–6 mm, slender, straight, or often curved at mid-height, distinctly clavate at base; surface entirely covered by an abundant whitish veil, forming or not a ring-like zone, on a vinaceous to reddish-brown or beige-brown ground visible by detersion; cortina white, loosening, early appressed. Context fairly thick at the centre, vinaceous, reddish-brown to brown when moist, and whitish and greyish when dry. Smell indistinct to slightly raphanoid or of cedar wood when cut. Taste mild. KOH reaction: brownish-black on the pileus surface and context. Spore print not obtained.
Spores (7) 7.5–10 (10.5) × (4.75) 5.25–5.5 (6.25) µm, yellowish-ochraceous in KOH, ellipsoid, pruniform to subamygdaliform, usually with strong ornamentation made of truncate warts up to 1.25 µm high, denser at the apex, with a visible apicular depression. Basidia 24–40 × 7.5–11 µm, tetrasporic. Sterile marginal cells, 5–18 µm wide, cylindric-clavate or paddle-like, sometimes difficult to see but always very abundant, at least locally, and septate. Pileipellis made of three layers of hyphae, reaching respectively 1.5–4 µm, up to 16 µm, and up to 32 µm wide, with coarse dark yellow encrusting pigment, sometimes forming broad patches. Clamps present at all septa.
Habitat and distribution: So far confirmed from alpine dwarf Salix microsylvae (S. herbacea, S. retusa, S. reticulata, and S. hastata) in the French and Swiss Alps above 2500 m asl and at lower elevations in northern Sweden, and on seashores of Finland, associated with S. repens or S. phylicifolia. Probably widespread in boreoarctic and alpine areas with Salix spp., but likely often confused with C. inops.
Notes: Identifying a member of the “Cortinarius inops complex” is not too difficult in the field because of their shared dark basidiomata, more or less abundant whitish veil, and frequent pinkish-lilac or vinaceous tinges of the flesh. However, it remains challenging to unambiguously resolve them solely on morphological bases. Consistently, the present species was first identified [14] as a “group 3” unnamed ITS and rpb2 lineage very close to, but phylogenetically distinct from, two lineages including the lectotype of C. inops J. Favre [12] and the holotype of C. suberythrinus Moën.-Locc., respectively. Concomitantly, published in the famous type study “Mission Impossible“ [2] was an ITS sequence of the holotype of C. tenebricus J. Favre, that belongs in sect. Verni, close to C. suberythrinus. The combined analysis of the two datasets (Figure 1) supports a complex of three cryptic or semi-cryptic species that includes C. inops (syn. C. tenebricus), C. suberythrinus (syn. C. vernus var. nevadavernus Suár.-Sant. & A. Ortega, ? syn. C. erythrinus Fr.), and C. infidus sp. nov. that was not rare in our prospected area of the French Alps. C. infidus differs from C. inops at the ITS locus by four nucleotide changes (2 SNPs + two indels) and at the rpb2 locus by 14 SNPs; it differs from C. suberythrinus by two nucleotide changes (1 SNP + one indel) only at the ITS locus but by 11 SNPs at the rpb2 locus [14].
Ecologically, C. infidus differs from C. suberythrinus by its arctic–alpine distribution, so far only recorded in association with Salix spp., whilst C. suberythrinus is more ubiquitous but not known to be associated with Salix. Cortinarius infidus and C. inops seem to share the same ecological niches and both were even observed once on the same spot (Col de l’Iseran, 23 August 2018). Such sympatric occurrence of two populations with fixed SNPs at two distinct loci, with no sign of gene flow (e.g., heteromorphisms at the variable sites) strongly supports the existence of a tight reproductive barrier between them and subsequently supports two distinct species. The analysis of our 10 collections belonging to this complex pointed spore length as a possible (subtle) diagnostic criterion to distinguish C. infidus and C. inops by non-molecular tools. Indeed, although the average length values are not much different between the two species (7.5–10 µm vs. 7–9,5 µm, respectively), we observed that the number of spores reaching 10–10.5 µm was significantly higher in C. infidus.
Other morphological features claimed by past authors to display taxonomic value within the complex, like the presence/absence of cheilocystidia [3], the pinkish colour of the flesh [6,8], or the smell of cedar wood [31], were not supported here as a diagnostic to separate the two species. Cheilocystidia may be overlooked if only a portion of the lamella is observed without scrutiny, and these sterile elements are more difficult to detect on exsiccata, as they are often collapsed. All our personal collections of C. infidus and C. inops displayed conspicuous cheilocystidia. The reported lack of these sterile elements in C. tenebricus by Favre himself and by Horak on the same material [3,12] illustrates the challenging detection of such a “key feature” emphasized for instance by Bon [32]. Cheilocystidia are also present in C. oreobius J. Favre [3,12], a morphologically and ecologically closely related species with slightly different spores, but whose phylogenetic identity has not yet been established. Cortinarius pusillus F.H. Møller might be a candidate for an earlier, but illegitimate name for C. infidus. This species described from the Faeroe Islands was considered for instance by Moser [33], but not by Bon [10], as conspecific with C. inops.
Cortinarius saniosopygmaeus Armada, Bellanger & P.-A. Moreau, sp. nov. MycoBank MB849753. (Figure 4A,B)
Etymology: Latin, evoking sect. Saniosi, which this species belongs to, and Cortinarius phaeopygmaeus, which it resembles morphologically.
Type material: FRANCE, Savoie, Peisey-Nancroix, Col de la Chal, 2500 m asl, among Salix herbacea, 17 August 2010, E. Armada & F. Armada (LY FA1724, holotype). GenBank ITS OR420003.
Diagnosis: A perfect lookalike of the ubiquitous C. saniosus or of its alpine synonym C. chrysomallus, with larger spores reaching 11 × 7 µm and fruiting in Salix herbacea carpets.
Pileus 7–20 mm in diameter, conical-convex then convex-flattened, with a small obtuse but fairly pronounced umbo, attenuated on older specimens; surface dry in early stages, dark reddish-brown gradually darker at the centre, not striate even when moist, not distinctly hygrophanous, made slightly fibrillose by a sparse yellowish-ochre veil especially visible at the margin, and glabrous when old; margin incurved, incised at the end, rendered orange-yellow by the veil, then glabrous and almost concolorous to the rest of surface on adults. Lamellae up to 3.5 mm wide, somewhat ventricose, broadly adnate to irregularly uncinate, sometimes subdecurrent, subdistant (17–23 reaching the stipe, one [-two] short plural per lamella), thin, pale beige-orange then rusty-orange; edge entire, subconcolorous to the face. Stipe 10–19 × 1–3 mm, straight or curved, and never fistulose; surface rendered entirely fibrillous by a persistent yellow veil, often forming a distinct ring-like zone at the lower third, showing a brownish ground colour when handled. Context not very thick, reddish-brown to dirty yellowish-white in the pileus. Smell not detected. Taste mild. KOH reaction: black, immediate on the pileipellis and context. Spore print not obtained.
Spores 9–11 (11.5–12.25) × (6) 6.5–7 (7.5) µm, yellowish-ochraceous in KOH, ellipsoid to subamygdaliform, with a small apicular depression, moderately verrucose, more coarsely at the apex. Basidia 28–39 × 8.5–11.5 µm, cylindrical or narrowly clavate, and tetrasporic. Sterile marginal cells very variable, locally abundant to nearly absent, cylindrical or racket-shaped, septate, and 7–10 µm wide. Pileipellis and trichocutis made of a layer of erected hyphae 1–3 µm wide, covering broader hyphae reaching 8–10 µm wide, and coarsely incrusted by a dark yellow to reddish epiparietal pigment. Subpellis a layer of allantoid hyphae reaching 28 µm wide, slightly less pigmented or encrusted than in the pileipellis. Clamps present at all septa.
Habitat and distribution: So far only confirmed from alpine Salix herbacea thickets of Austria, France, and Switzerland.
Additional material: AUSTRIA, Ötztal, alpine Salix herbacea snowbed, 22 August 1990, E. & K. Bendiksen, EKB 90.0822 (as C. chrysomallus, not studied), GenBank ITS DQ102656. SWITZERLAND, Graubünden. Scuol, Lai Sesvenna, near S. herbacea, 2665 m asl, 25 August 2019, K. Kokkonen, KK 35/19 (not studied), GenBank ITS MN841233; ibid., Val Sesvenna, near S. herbacea, 2525 m asl, 14 August 2017, K. Kokkonen, KK 306/17 (not studied), 307/17 (not studied), GenBank ITS MN841232; ibid., 360/17 (not studied); ibid., 17 August 2017, KK 283/17 (not studied), 316/17 (not studied). Cortinarius saniosus: FRANCE, Savoie, Peisey-Nancroix, Lac Marlou, 2500 m asl, among Salix herbacea, 21 August 2008, F. Armada, E. Armada & N. Van Vooren, FA 1069, GenBank ITS OR420002; Bonneval-sur-Arc, col de l’Iseran, 2650 m asl, among Salix retusa, 23 August 2018, F. Armada, FA 4317, GenBank ITS OR420005. Cortinarius subsaniosus: FRANCE, Savoie, Peisey-Nancroix, Lac Marlou, 2500 m asl, among Salix herbacea, 19 August 2010, F. Armada, E. Armada, A. Bidaud & E. Bidaud, FA 1751/AB 10-08-57, GenBank ITS OR420004.
Notes: The present lineage has been known for a while but has not been formally named so far. In a landmark taxonomic revision of the C. saniosus (Fr.) Fr. complex, Lindström et al. [34] identified a group of four sequences in a so-called “clade 2”, sister to C. saniosus. Three of these sequences were later shown [14,35] to belong to C. subsaniosus Liimat. & Niskanen, a perfect lookalike of C. saniosus, with just slightly larger spores and a possible narrower distribution in lowland or coastal sandy areas. The fourth sequence in clade 2, GenBank DQ102656, originated from an Austrian alpine collection with Salix herbacea and was initially identified (and confirmed by M. Moser) as C. chrysomallus Lamoure [36]. Six additional collections were cited from similar ecosystems in Switzerland, with ITS sequences identical to DQ102656 [14]. Despite having pointed out some morphological differences in comparison with C. saniosus and C. subsaniosus, Kokkonen [14] refrained from formally naming this S. herbacea-associated alpine Saniosi. The three species co-occurred in our prospecting areas with Salix herbacea in a higher alpine zone, and we could compare them in detail for this study.
Cortinarius subsaniosus has not been recorded in the alpine zone to date. Our above-listed collections of C. subsaniosus and of C. saniosus were determined in the field as C. saniosus or C. chrysomallus, which were both expected in alpine snowbeds according to the usual literature [7,8,9,10]. Collection FA 1724 (C. saniosopygmaeus) was thought to represent C. phaeopygmaeus J. Favre, because of slightly larger spores than the two previous Saniosi species. The latter name was considered one of the less-well-characterized and interpretable alpine Cortinarius species [10,12,36] till its lectotype [12] was successfully sequenced and revealed that C. phaeopygmaeus is a member of the sect. Flexipedes [37], excluding conspecificity with the present species.
Spore size may be a key feature to disentangle this species complex: FA1724 displayed spores reaching 11 × 7 µm, whilst our collections of C. saniosus displayed spores measuring (8) 8.5–10 × 5–6.5 µm in accordance with previous studies [14,34,35]. Our unique collection of C. subsaniosus displayed similar spores, measuring 7.5–10 (10.5) × 5.5–6.5 µm, thus smaller than those published in the protologue (9.5–11 × 6–7 µm, MV = 10.2 × 6.1 µm [35]) but in accordance with Kokkonen (8–10 × 5.5–6 µm, MV = 9.1 × 5.7 µm), suggesting some phenotypic plasticity within C. subsaniosus, already reported at the macroscopic level [14].
Although spores of more sequenced collections of the three species should be carefully measured to confirm their respective range of variations, we here define C. saniosopygmaeus sp. nov. as a strictly Salix herbacea-associated species with distinctly larger spores on average than other alpine Saniosi.
3.2. Type Revisions and Updates
Cortinarius caesionigrellus Lamoure, Trav. Sci. Parc Natl. Vanoise 9: 82 (1978) (Figure 5).
Material studied: FRANCE, Savoie, Bonneval-sur-Arc, behind l’Ouille-des-Reys, 2650 m, among Salix herbacea, 22 August 1971, D. Lamoure (LY L. 71-51, holotype), GenBank ITS OR419961. ITALY, Lombardy, Sondrio, Valfurva, passo Gavia, 2550 m asl, in an alpine meadow of Salix herbacea and Alchemilla pentaphyllea, 46°21′25.5″ N 10°30′02.7″ E, 18 August 2021, A. Fellin (TR gmb 01247), GenBank ITS OR420018; ibid. 31 July 2018 (TR gmb 01248), not sequenced.
Pileus (6) 8–25 mm in diameter, hemispherical to convex-appressed, in young specimens also campanulate, with central umbo barely outlined; inflexed margin, finely incised; cuticle dry, fibrillose, with a tendency to fissure radially or more irregularly towards the centre, covered by white-greyish araneous veil remnants or small appressed scales in young specimens, more abundant and persistent towards the margin, blackish-brown when wet, chestnut-brown, and lighter brown towards the margin in dry weather. Lamellae adnate-emarginate, distant, broad, sinuous, finely veined on the faces, brownish but with conspicuous and uniform lilac-purple tinges; edge entire or weakly indented, paler, and greyish; interspersed with short lamellulae. Stipe 8–25 (30) mm, cylindrical, sometimes curved, attenuated or barely clavate at the base or enlarged in the middle, striated, fibrillose, and covered with scattered or irregularly arranged greyish-white veil remnants; ground colour brownish and subconcolorous to the pileus; stipe base and mycelial felt occasionally with bluish hues. Context concolorous or subconcolorous with the pileus, darker and sometimes with a bluish tinge towards the stipe base and in the cortex; slight smell of Pelargonium.
Spores (8.1–) 8.5–9.1–9.6 (–10.2) × (5.4–) 5.6–5.9–6.2 (–6.5) µm, Q = (1.3–) 1.44–1.54–1.65 (–1.78), yellowish-ochraceous in KOH, dextrinoid, broadly ellipsoid to subovoid with rather variable profile, and finely verrucose; ornamentation consisting of low punctiform or trapezoidal warts, often larger and coalescent at the apex. Basidia 28–42 × 8–11 µm, claviform, hyaline or brownish in KOH, and mostly tetrasporic but with the presence of sporadic trisporic and bisporic basidia. Sterile marginal cells trivial, cylindric-clavate, basidioliform, interspersed between basidia, hyaline, and 13–28 × 4–8 µm. Pileipellis consisting of a thin layer of hyaline or at most yellowish hyphae 2–4 µm wide (suprapellis) covering a layer of parallel cylindrical hyphae 3–9 µm wide (mediopellis); subpellis made of broader, ventricose, short septate hyphae 15–20 µm wide; strongly encrusting, brownish pigment as coarse granulations in the pileipellis, and as small plaques or clustered granules in the subpellis. Clamps present at all septa.
Habitat and distribution: Reported from several alpine localities in France and Switzerland above 2300 m asl and from the Swedish Lapland, in snowbeds with Salix herbacea, but so far only confirmed from France and Italy, always with Salix herbacea and occasional Alchemilla pentaphyllea nearby.
Notes: This species was described by D. Lamoure as a rare but easily diagnosable species in the field, by the blue colour of the young lamellae and basal mycelium, combined with the greyish veil that persists long at the pileus margin, and its ecology restricted to high alpine snowbeds [8]. Since then, it has been recorded only twice, from Switzerland [38] and Italy [39]. An additional collection from M. Maletti (Italy), is reported and illustrated online [40]. However, without molecular support, there is no evidence that any of these collections are conspecific with Lamoure’s taxon. None of the recent revisions of the subgenus [2,14] included this species.
To complete our survey of alpine Agaricales of the Vanoise area [40], we succeeded in finding at LY and sequencing the holotype collection of C. caesionigrellus (L. 71-51), here confirmed as a member of the sect. Flexipedes (Figure 1). The phylogenetically closest species is the non-alpine species C. umbrinolutescens Reumaux [2], with four SNPs + eight indels separating the two ITS type sequences. No sequence with more than 97.9% identity with that of L. 71-51 is currently (5 August 2023) available in GenBank or UNITE, suggesting that C. caesionigrellus is probably a rare species at the global scale. Serendipitously, A. F. deferred to J.-M. B.’s expertise on the sequence of a small alpine Telamonia collected under Salix herbacea in the Italian Alps, which did not match anything by Blast in GenBank. This sequence turned out to differ from that of L. 71-51 by a unique SNP and is thus considered here as representing the first DNA-confirmed record of this species since its original description (Figure 1). This unexpected finding allows us to update Lamoure’s original diagnosis and to reliably illustrate this species for the first time.
Cortinarius flexipes var. brachyspermus (Lamoure) Bellanger, comb. nov. MycoBank MB849754
Basionym: Cortinarius paleifer var. brachyspermus Lamoure, arctic and alpine Mycology, II: 258 (1987)
Material studied: FINLAND, Lapland, amongst Polytrichum norvegicum and Sibbaldia procumbens under Salix herbacea, 1972, D. Lamoure (LY L. 72-64, holotype). GenBank ITS OR419960.
Notes: The ITS sequence of the holotype of Cortinarius paleifer var. brachyspermus, which we could generate from the original material at LY, turned out to be almost identical (one SNP + two indels) to that of the neotype of C. flexipes (Pers.) Fr., in sect. Flexipedes (Figure 1). This finding supports the original treatment of the present taxon as a variety of C. paleifer Svrček, the latter name being considered a late synonym of C. flexipes by several authors [41,42]. This synonymy was recently [2] confirmed by sequencing the type collections of both taxa. As a result, and because of the morphological and ecological singularities of this alpine taxon described in detail by Lamoure [35,43], we here recombine this variety under C. flexipes. No recent collection or illustration of this variety is known to us.
Thaxterogaster dovrensis (Brandrud) Bellanger, comb. & stat nov. MycoBank MB849755 (Figure 6).
Basionym: Cortinarius argenteolilacinus var. dovrensis Brandrud, in Brandrud et al., Mycol. Progr., 17 (12): 1346 (2018)
Material studied: FRANCE, Peisey-Nancroix, along the GR5 path to the lake of La Plagne, 2050 m asl, alpine meadow with Helianthemum nummularium and Salix retusa, close to Dryas octopetala and Polygonum viviparum, 25 August 2018, F. Armada (LY FA4340). GenBank ITS OR419956.
Notes: In their revision of sect. Riederi, Brandrud et al. [44] taxonomically treated C. argenteolilacinus M.M. Moser as an inclusive species with two varieties: var. argenteolilacinus for mainly nemoral-montane Fagus (-Tilia)-associated populations and a newly introduced var. dovrensis Brandrud for mainly northern, subarctic Betula-associated populations. Despite the fact that translating phylogeny into taxonomy is largely a matter of human choice, this one is a bit surprising because the two sister clades are well-supported and do not display any sign of gene flow. Moreover, as the authors rightly pointed out, they are associated by a combination of ecogeographical, morphological, and anatomical conspicuous differences that would largely support the species rank for these two sister taxa. In their comments, the authors justified their choice of the varietal rank over the subspecies rank (“due to geographical differences, these taxa might also be treated as subspecies, but we think it is wise to stick to only one infraspecific rank (subspecies is nowadays very little applied in agaric taxonomy”) [44], but never evoked a possible species rank for dovrensis. Most likely, their conservatism resulted from the short phylogenetic distance between the two clades: “the taxon is phylogenetically very closely related to C. argenteolilacinus var. argenteolilacinus and differs only in two to three substitution and indel positions in the ITS region”. One could argue that a number of genuine Cortinarius species (i.e., differing by clear and not overlapping morphological or ecological features) differ by less than three nucleotide changes, especially in the subgenus Telamonia [2,14]. Others may argue that phylogeny is not an alternative way but a tool to serve taxonomy, and that prioritizing nucleotide differences at a single locus over morpho-anatomical, biogeographical, or ecological stable associated features to assign a taxonomic rank is actually not so conservative.
The cases where infraspecific ranks have been proposed or maintained by sequence data are actually not so numerous in Cortinarius and they consisted of either distinct morphotaxa with no associated sequence difference (e.g., C. atrovirens subsp. ionochlorus (Maire) Vizzini & Gasparini [45]), or cryptic/semi-cryptic species with a limited number of nucleotide differences (e.g., C. subturibulosus var. bombycinus (Mahiques & Burguete) Suár.-Sant. & A. Ortega [23]). It should be noted that many of such cryptic/semi-cryptic phylospecies are later raised to the species rank, upon deeper sampling that unveils overlooked morpho-anatomic diagnostic features, or upon sequencing additional loci that unveils unreasonably long phylogenetic distances for two infraspecific taxa. Cortinarius argenteolilacinus var. dovrensis does not fit into any of these categories and deserves, in our opinion, the species rank.
In 2022, “Taming the beast […]” was published [1], a breakthrough paper splitting Cortinarius into 10 smaller genera. This new systematics is disruptive in many respects, but it is grounded in unprecedented phylogenomic data that grant it solid evolutionary bases. In this study, C. argenteolilacinus and related taxa so far treated in sect. Riederi, nest in Thaxterogaster, a genus previously including only sequestrate mushrooms [46] but shown to phylogenetically represent a wider and distinct lineage within Cortinariaceae, sister to all other newly introduced genera [1]. Without explanation but likely applying the art. 21.2 of the current version of the Code of Nomenclature (“Code Shenzhen” [47]), Liimatainen et al. [1] changed the name of the sect. Riederi to sect. Riederorum, and further introduced subgen. Riederorum (erroneously labelled as T. subgenus Riederi in Figure 2) to distinguish this lineage from phylogenetically and morphologically distantly related taxa from subgenera e.g., Multiformes and Scauri. The authors have proceeded to the most relevant recombinations of species, including Thaxterogaster argenteolilacinus (M.M. Moser) Niskanen & Liimat.). We here follow this systematics and meanwhile raise Brandrud’s taxon to the species rank it deserves according to the above-mentioned arguments.
The collection illustrated here is the first French record for this species, as well as its first published observation in the alpine zone.
F.A., J.-M.B. and P.-A.M. designed the study; F.A. and A.F. collected and studied the samples macro- and microscopically, and wrote the descriptions; J.-M.B. sequenced all the cited collections; and J.-M.B. and P.-A.M. performed the phylogenetic analyses. All authors have read and agreed to the published version of the manuscript.
Not applicable.
Not applicable.
All DNA sequences generated in this study are deposited in GenBank. Collecting data are integrated into the MycoflAURA regional databases on fungal records (
We especially thank Mélanie Thiébaut and Blandine Bärtschi (LY) for having arranged the authors’ visits to D. Lamoure’s fungal collections and allowing DNA extractions from this material. We are grateful to Esteban Armada, André and Espérance Bidaud, and Nicolas Van Vooren for their field collaboration in alpine samplings, and Katri Kokkonen (Finland) for sharing unpublished data and useful comments on arctic collections. DNA extraction and PCR amplifications were performed in the GEMEX facility of the CEFE (head: Marie-Pierre Dubois).
The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.
Footnotes
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.
Enlarge this image.Figure 1. Partial midpoint rooted ML phylogenetic reconstruction of subgen. Telamonia from ITS sequences. In bold: sequences generated for this study.
Enlarge this image.Figure 2. Cortinarius dryadophilus LY FA4330T. (A) Basidiomata in situ. (B) Basidiospores. Bar: 10 mm (A), 10 µm (B). Credits: F. Armada.
Enlarge this image.Figure 3. Cortinarius infidus (A,B,D) LY FA4276T. (C,E,F) FA4281. (A,C) Basidiomata in situ. (B) Basidiospores. (D–F) Cheilocystidia. Bar: 10 mm (A,C), 10 µm (B,D–F). Credits: F. Armada.
Enlarge this image.Figure 4. Cortinarius saniosopygmaeus LY FA1724T. (A) Basidiomata in situ. (B) Basidiospores. Bar: 10 mm (A), 10 µm (B). Credits: F. Armada.
Enlarge this image.Figure 5. Cortinarius caesionigrellus TR gmb 01247, basidiomata in situ. Bar: 10 mm. Credit: A. Fellin.
Enlarge this image.Figure 6. Thaxterogaster dovrensis (LY FA4340). (A) Basidiomata in situ. (B) Basidiospores. Bar: 10 mm (A), 10 µm (B). Credits: F. Armada.
References
1. Liimatainen, K.; Kim, J.T.; Pokorny, L.; Kirk, P.M.; Dentinger, B.; Niskanen, T. Taming the beast: A revised classification of Cortinariaceae based on genomic data. Fungal Div.; 2022; 112, pp. 89-170. [DOI: https://dx.doi.org/10.1007/s13225-022-00499-9]
2. Liimatainen, K.; Niskanen, T.; Dima, B.; Ammirati, J.F.; Kirk, P.M.; Kytövuori, I. Mission impossible completed: Unlocking the nomenclature of the largest and most complicated subgenus of Cortinarius, Telamonia. Fungal Div.; 2020; 104, pp. 291-331. [DOI: https://dx.doi.org/10.1007/s13225-020-00459-1]
3. Favre, J. Les champignons supérieurs de la zone alpine du Parc national Suisse. Ergebn. Wiss. Untersuch. Schweizerisches. Nationalparks; 1955; 5, pp. 1-212.
4. Brunner, I.; Frey, B.; Hartmann, M.; Zimmermann, S.; Graf, F.; Suz, L.M.; Niskanen, T.; Bidartondo, M.I.; Senn-Irlet, B. Ecology of alpine macrofungi—Combining historical with recent data. Front. Microbiol.; 2017; 26, 2066. [DOI: https://dx.doi.org/10.3389/fmicb.2017.02066]
5. Kühner, R.; Lamoure, D. Agaricales de la zone alpine. Introduction. Bull. Soc. Mycol. France; 1970; 86, pp. 875-880.
6. Kühner, R.; Lamoure, D. Catalogue des Agaricales (Basidiomycètes) de la zone alpine du Parc national de la Vanoise et des régions limitrophes. Trav. Sci. Parc natl Vanoise; 1986; 15, pp. 103-187.
7. Lamoure, D. Agaricales de la zone alpine. Genre Cortinarius Fr. Sous-genre Telamonia (Fr.) Loud. 1re partie. Trav. Sci. Parc. Natl. Vanoise; 1977; 8, pp. 115-146.
8. Lamoure, D. Agaricales de la zone alpine. Genre Cortinarius Fr. Sous-genre Telamonia (Fr.) Loud. suite (I). Trav. Sci. Parc. Natl. Vanoise; 1978; IX, pp. 77-101.
9. Bon, M. Quelques nouveaux taxons de la flore mycologique alpine. Bull. Trim. Féd. Mycol. Dauphiné-Savoie; 1985; 25, pp. 23-30.
10. Bon, M. Clé analytique des Cortinaires alpins (avec quelques incursions dans la rhodoraie et l'aulnaie verte). Doc. Mycol.; 1992; 22, pp. 43-67.
11. Fellner, R.; Landa, J. Some species of Cortinariaceae and Russulaceae in the alpine belt of the Belaer Tatras. Bibl. Mycol.; 1993; 150, pp. 33-37.
12. Horak, E. Revision der von J. Favre (1955) aus der Region des Schweizer Nationalparks beschriebenen alpinen Arten von Cortinarius subgen. Telamonia (Agaricales). Candollea; 1987; 42, pp. 771-803.
13. Moreau, P.-A. À la découverte des champignons de zone alpine…. Bull. Mycol. Bot. Dauphiné-Savoie; 2002; 166, pp. 5-61.
14. Kokkonen, K. Diversity of boreal small species of Cortinarius subgenus Telamonia with Salix. Karstenia; 2020; 58, pp. 60-117. [DOI: https://dx.doi.org/10.29203/ka.2020.489]
15. Henriot, A.; Cheype, J.-L. Piximètre: La Mesure de Dimensions sur Images. Version 5.10. Available online: http://www.piximetre.fr (accessed on 1 August 2023).
16. White, T.J.; Bruns, T.; Lee, S.; Taylor, J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols: A Guide to Methods and Applications; Innis, M.A.; Gelfand, D.H.; Sninsky, J.J.; White, T.J. Academic Press: New York, NY, USA, 1990; pp. 315-322. [DOI: https://dx.doi.org/10.1016/B978-0-12-372180-8.50042-1]
17. Gardes, M.; Bruns, T.D. ITS primers with enhanced specificity for Basidiomycetes—Application to the identification of mycorrhizae and rusts. Mol. Ecol.; 1993; 2, pp. 113-118. [DOI: https://dx.doi.org/10.1111/j.1365-294X.1993.tb00005.x] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/8180733]
18. Moreau, P.-A.; Bellanger, J.-M.; Lebeuf, R.; Athanassiou, Z.; Athanasides, A.; Lambert, H.; Schwarz, C.; Larsson, E.; Loizides, M. Hidden diversity uncovered in Hygrophorus sect. Aurei (Hygrophoraceae), including the Mediterranean H. meridionalis and the North American H. boyeri, spp. nov. Fungal Biol.; 2018; 122, pp. 817-836. [DOI: https://dx.doi.org/10.1016/j.funbio.2018.04.009]
19. Edgar, R.C. MUSCLE: Multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res.; 2004; 32, pp. 1792-1797. [DOI: https://dx.doi.org/10.1093/nar/gkh340] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/15034147]
20. Dereeper, A.; Guignon, V.; Blanc, G.; Audic, S.; Buffet, S.; Chevenet, F.; Dufayard, J.-F.; Guindon, S.; Lefort, V.; Lescot, M. et al. Phylogeny.fr: Robust phylogenetic analysis for the non-specialist. Nucleic Acids Res.; 2008; 1, pp. W465-W469. [DOI: https://dx.doi.org/10.1093/nar/gkn180] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/18424797]
21. Anisimova, M.; Gil, M.; Dufayard, J.-F.; Dessimoz, C.; Gascuel, O. Survey of branch support methods demonstrates accuracy, power, and robustness of fast likelihood-based approximation schemes. Syst. Biol.; 2011; 60, pp. 685-699. [DOI: https://dx.doi.org/10.1093/sysbio/syr041]
22. Bellanger, J.M.; Moreau, P.-A.; Corriol, G.; Bidaud, A.; Chalange, R.; Dudova, Z.; Richard, F. Plunging hands into the mushroom jar: A phylogenetic framework for Lyophyllaceae (Agaricales, Basidiomycota). Genetica; 2015; 143, pp. 169-194. [DOI: https://dx.doi.org/10.1007/s10709-015-9823-8]
23. Suárez-Santiago, V.N.; Ortega, A.; Peintner, U.; López-Flores, I. Study on Cortinarius subgenus Telamonia section Hydrocybe in Europe, with especial emphasis on Mediterranean taxa. Mycol. Res.; 2009; 113, pp. 1070-1090. [DOI: https://dx.doi.org/10.1016/j.mycres.2009.07.006]
24. Armada, F.; Bellanger, J.-M. Cortinarius cistocastaneus sp. nov., un nouveau cortinaire pour la section Castanei récolté sous Cistus ladanifer. Bull. Mycol. Bot. Dauphiné-Savoie; 2023; 248, pp. 45-56.
25. Crous, P.W.; Boers, J.; Holdom, D.; Steinrucken, T.V.; Tan, Y.P.; Vitelli, J.S.; Shivas, R.G.; Barrett, M.; Boxshall, A.-G.; Broadbridge, J. et al. Fungal Planet description sheets: 1383–1435. Persoonia; 2022; 48, pp. 261-371. [DOI: https://dx.doi.org/10.3767/persoonia.2022.48.08]
26. Orton, P.D. Notes of British Agarics VIII. Trans. Br. Mycol. Soc.; 1984; 41, pp. 565-624.
27. Moënne-Loccoz, P.; Reumaux, P.; Henry, R. Atlas des Cortinaires, Pars I; Fédération Mycologique Dauphiné-Savoie: Marlioz, France, 1990.
28. Bidaud, A.; Carteret, X.; Eyssartier, G.; Moënne-Loccoz, P.; Reumaux, P. Atlas des Cortinaires. Pars XI; Fédération Mycologique Dauphiné-Savoie: Marlioz, France, 2001.
29. Moser, M.M.; McKnight, K.H. Fungi (Agaricales, Russulales) from the alpine zone of Yellowstone national Park and the Beartooth Mountains with special emphasis on Cortinarius. Arctic and Alpine Mycology II; Laursen, G.A.; Ammirati, J.F.; Redhead, S.A. Environmental Science Research Springer: Boston, MA, USA, 1987; Volume 34, pp. 299-317. [DOI: https://dx.doi.org/10.1007/978-1-4757-1939-0_20]
30. Bellanger, J.M.; Bidaud, A.; Moreau, P.-A. Cortinarius subturibulosus: «Illumina»-tion d’un champion de l’adaptation. J. JEC; 2021; 23, pp. 3-15.
31. Corriol, G. Quelques Telamonia alpins des Pyrénées. Bull. Mycol. Bot. Dauphiné-Savoie; 2015; 218, pp. 69-89.
32. Bon, M. Mycologie alpine, 24/27 août 1989—“Les Ménuires”. Bull. Trim. Féd. Mycol. Dauphiné-Savoie; 1990; 29, pp. 25-30.
33. Moser, M.M. Studies on North American Cortinarii. III. The Cortinarius flora of dwarf and shrubby Salix associations in the alpine zone of the Windriver Mountains, Wyoming, (USA). Sydowia; 1993; 45, pp. 275-306.
34. Lindström, H.; Bendiksen, E.; Bendiksen, K.; Larsson, E. Studies of the Cortinarius saniosus (Fr.: Fr.) Fr. complex and a new closely related species, C. aureovelatus (Basidiomycota, Agaricales). Sommerfeltia; 2008; 31, pp. 139-159. [DOI: https://dx.doi.org/10.2478/v10208-011-0008-2]
35. Hyde, K.D.; Dong, Y.; Phookamsak, R.; Jeewon, R.; Bhat, D.J.; Jones, E.B.G.; Liu, N.-G.; Abeywickrama, P.D.; Mapook, A.; Wei, D. et al. Fungal diversity notes 1151–1276: Taxonomic and phylogenetic con-tributions on genera and species of fungal taxa. Fungal Div.; 2020; 100, pp. 5-277. [DOI: https://dx.doi.org/10.1007/s13225-020-00439-5]
36. Lamoure, D. Notes critiques sur des Telamonia de la zone alpine (Genre Cortinarius Fr., Agaricales). I—Quelques espèces à odeur de Pelargonium. Bull. Mens. Soc. Linn. Lyon; 1991; 60, pp. 152-156. [DOI: https://dx.doi.org/10.3406/linly.1991.10933]
37. Kokkonen, K. Type studies of Cortinarius phaeopygmaeus and C. rusticellus, and a new salicticolous species C. chrysophilus. Karstenia; 2023; 61, pp. 1-10. [DOI: https://dx.doi.org/10.29203/ka.2023.517]
38. Breitenbach, J.; Kränzlin, F. Champignons de Suisse. Champignons à Lames, 3ème Partie. Cortinariaceae; Tome 5; Mykologia: Lucerne, Switzerland, 2000; 340p.
39. Consiglio, G.; Antonini, D.; Antonini, M. Il Genre Cortinarius in Italia; Volume 5. Edizione AMB Centro Studi Micologici: Trento, Italy, 2007.
40. Associazione Micologica Bresadola. Guppo du Pesaro. Cortinarius caesionigrellus. Available online: http://www.ambpesaro.it/portfolio-item/cortinarius-caesionigrellus (accessed on 1 August 2023).
41. Arnold, N. Boekbespreking: Morphologisch-Anatomische und Chemische Untersuchungen an der Untergattung Telamonia (Cortinarius. Agaricales); Libri Botanici 7 IHW-Verlag: Eching, Germany, 1993.
42. Brandrud, T.E.; Lindström, H.; Marklund, H.; Melot, J.; Muskos, S. Cortinarius, Flora Photografica; 4th ed. Cortinarius HB: Matfors, Sweden, 1998.
43. Lamoure, D. Agaricales de la zone alpine. Genus Cortinarius, subgenus Telamonia, part III. Arctic and Alpine Mycology II; Environmental Science Research Laursen, G.A.; Ammirati, J.F.; Redhead, S.A. Springer: Boston, MA, USA, 1987; Volume 34, pp. 255-259. [DOI: https://dx.doi.org/10.1007/978-1-4757-1939-0_16]
44. Brandrud, T.E.; Schmidt-Stohn, G.; Liimatainen, K.; Niskanen, T.; Frøslev, T.G.; Soop, K.; Bojantchev, D.; Kytövuori, I.; Jeppesen, T.S.; Bellù, F. et al. Cortinarius sect. Riederi: Taxonomy and phylogeny of the new section with European and North American distribution. Mycol. Prog.; 2018; 17, pp. 1323-1354. [DOI: https://dx.doi.org/10.1007/s11557-018-1443-0]
45. Vizzini, A. Novitates. Miscellanea. Rivista Micol.; 2008; 51, pp. 63-66.
46. Peintner, U.; Bougher, N.L.; Castellano, M.A.; Moncalvo, J.-M.; Moser, M.M.; Trappe, J.M.; Vilgalys, R. Multiple origins of sequestrate fungi related to Cortinarius (Cortinariaceae). Am. J. Bot.; 2001; 88, pp. 2168-2179. [DOI: https://dx.doi.org/10.2307/3558378]
47. Turland, N.J.; Wiersema, J.H.; Barrie, F.R.; Greuter, W.; Hawksworth, D.L.; Herendeen, P.S.; Knapp, S.; Kusber, W.H.; Li, D.-Z.; Marhold, K. International Code of Nomenclature for Algae, Fungi, and Plants (Shenzhen Code) Adopted by the Nineteenth International Botanical Congress Shenzhen, China, July 2017. Regnum Vegetabile 159; Koeltz Botanical Books: Glashütten, Germany, 2018; [DOI: https://dx.doi.org/10.12705/Code.2018]
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
Details
; Armada, François 2 ; Fellin, Alessandro 3 ; Pierre-Arthur Moreau 4
1 CEFE, CNRS, Univ. Montpellier, EPHE, IRD, INSERM, 1919, Route de Mende, CEDEX 5, F-34293 Montpellier, France
2 203, Montée Saint-Mamert-le-Haut, F-38138 Les Côtes-d’Arey, France;
3 Via G. Canestrini 10/B, I-38028 Novella, TN, Italy;
4 203, Montée Saint-Mamert-le-Haut, F-38138 Les Côtes-d’Arey, France;