Full Text

Turn on search term navigation

© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Background: Whole-genome sequencing (WGS) provides important information for the characterization, surveillance, and monitoring of antimicrobial resistance (AMR) determinants, particularly in cases of multi- and extensively drug-resistant microorganisms. We reported the results of a WGS analysis carried out on carbapenemases-producing Klebsiella pneumoniae, which causes hospital-acquired infections (HAIs) and is characterized by a marked resistance profile. Methods: Clinical, phenotypic, and genotypic data were collected for the AMR surveillance screening program of the University Hospital of Sassari (Italy) during 2020–2021. Genomic DNA was sequenced using the Illumina Nova Seq 6000 platform. Final assemblies were manually curated and carefully verified for the detection of antimicrobial resistance genes, porin mutations, and virulence factors. A phylogenetic analysis was performed using the maximum likelihood method. Results: All 17 strains analyzed belonged to ST512, and most of them carried the blaKPC-31 variant blaOXA-48-like, an OmpK35 truncation, and an OmpK36 mutation. Phenotypic analysis showed a marked resistance profile to all antibiotic classes, including β-lactams, carbapenems, aminoglycosides, fluoroquinolone, sulphonamides, and novel β-lactam/β-lactamase inhibitors (BL/BLI). Conclusion: WGS characterization revealed the presence of several antibiotic resistance determinants and porin mutations in highly resistant K. pneumoniae strains responsible for HAIs. The detection of blaKPC-31 in our hospital wards highlights the importance of genomic surveillance in hospital settings to monitor the emergence of new clones and the need to improve control and preventive strategies to efficiently contrast AMR.

Details

Title
A Whole-Genome Sequencing-Based Approach for the Characterization of Klebsiella pneumoniae Co-Producing KPC and OXA-48-like Carbapenemases Circulating in Sardinia, Italy
Author
Arcadia Del Rio 1   VIAFID ORCID Logo  ; Fox, Valeria 2 ; Muresu, Narcisa 3   VIAFID ORCID Logo  ; Sechi, Illari 4   VIAFID ORCID Logo  ; Cossu, Andrea 4 ; Palmieri, Alessandra 4   VIAFID ORCID Logo  ; Scutari, Rossana 2 ; Alteri, Claudia 2 ; Sotgiu, Giovanni 5   VIAFID ORCID Logo  ; Castiglia, Paolo 4   VIAFID ORCID Logo  ; Piana, Andrea 4   VIAFID ORCID Logo 

 Department of Biomedical Science, University of Sassari, 07100 Sassari, Italy; [email protected] 
 Department of Oncology and Hemato-Oncology, University of Milan, 20122 Milan, Italy; [email protected] (V.F.); [email protected] (R.S.); [email protected] (C.A.) 
 Department of Humanities and Social Sciences, University of Sassari, 07100 Sassari, Italy 
 Department of Medicine, Surgery, and Pharmacy, University of Sassari, 07100 Sassari, Italy; [email protected] (I.S.); [email protected] (A.C.); [email protected] (A.P.); [email protected] (P.C.); [email protected] (A.P.) 
 Clinical Epidemiology and Medical Statistics Unit, Department of Medical, Surgical and Experimental Medicine, University of Sassari, 07100 Sassari, Italy; [email protected] 
First page
2354
Publication year
2023
Publication date
2023
Publisher
MDPI AG
e-ISSN
20762607
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2869454072
Copyright
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.