Abstract

Filamentous fungus can produce raw-starch-degrading enzyme (RSDE) that efficiently degrades raw starch below starch gelatinization temperature. Employment of RSDE in starch processing can save energy. A key putative transcription factor PoxRsrA (production of raw-starch-degrading enzyme regulation in Penicilliumoxalicum) was identified to regulate RSDE production in P. oxalicum; however, its regulatory mechanism remains unclear. Here we show that PoxRsrA1434–1730 was the transcriptional activation domain, with essential residues, D1508, W1509 and M1510. SANT (SWI3, ADA2, N-CoR and TFIIIB)-like domain 1 (SANT1) bound to DNA at the sequence 5′-RHCDDGGD-3′ in the promoter regions of genes encoding major amylases, with an essential residue, R866. SANT2 interacted with a putative 3-hydroxyisobutyryl-CoA hydrolase, which suppressed phosphorylation at tyrosines Y1127 and Y1170 of PoxRsrA901–1360, thereby inhibiting RSDE biosynthesis. PoxRsrA1135–1439 regulated mycelial sporulation by interacting with Mediator subunit Med6, whereas PoxRsrA1440–1794 regulated RSDE biosynthesis by binding to Med31. Overexpression of PoxRsrA increased sporulation and RSDE production. These findings provide insights into the regulatory mechanisms of fungal RSDE biosynthesis.

Transcription factor PoxRsrA of Penicillium oxalicum is a nuclear protein containing two SANT domains with different functions, and regulates raw-starch-degrading enzyme production.

Details

Title
Regulation of fungal raw-starch-degrading enzyme production depends on transcription factor phosphorylation and recruitment of the Mediator complex
Author
Ning, Yuan-Ni 1 ; Tian, Di 1 ; Tan, Man-Li 2 ; Luo, Xue-Mei 2 ; Zhao, Shuai 1   VIAFID ORCID Logo  ; Feng, Jia-Xun 1   VIAFID ORCID Logo 

 Guangxi University, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Nanning, PR China (GRID:grid.256609.e) (ISNI:0000 0001 2254 5798); Guangxi University, Guangxi Research Center for Microbial and Enzyme Engineering Technology, Nanning, PR China (GRID:grid.256609.e) (ISNI:0000 0001 2254 5798); Guangxi University, College of Life Science and Technology, Nanning, PR China (GRID:grid.256609.e) (ISNI:0000 0001 2254 5798) 
 Guangxi University, College of Life Science and Technology, Nanning, PR China (GRID:grid.256609.e) (ISNI:0000 0001 2254 5798) 
Pages
1032
Publication year
2023
Publication date
2023
Publisher
Nature Publishing Group
e-ISSN
23993642
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2876186461
Copyright
© The Author(s) 2023. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.