Development of Flaxseed Submitted to Nitrogen Doses 1

Headnote

ABSTRACT

The flaxseed is little explored under Brazilian cultivation conditions, generating a gap in infonnation about the appropriate management of the crop, in the country. Hence, the objective was to find the appropriate dose of nitrogen for the development of the variables: technical length, yield length, main stem diameter, number of grains per pod. plant grain yield, and plant dry mass of two flaxseed varieties, brown and golden, in places with subtropical and tropical climate in Brazil. The treatments consisted of a two-factor 2x6 experiment, consisting of two flax varieties (brown and golden) and six nitrogen doses (0; 30; 60; 90; 120 and 150 kg ha1), in randomized blocks with four repetitions and three different enviromnents: Santa Maria and Frederico Westphalen, in the state of Rio Grande do Sul. and Viçosa. in the state of Minas Gerais. The brown variety obtained greater technical and yield length compared to the golden variety, on the other hand, the golden variety showed a higher number of grains per capsule and higher plant grain yield compared to the brown variety, in the three enviromnents. The plant dry mass increased linearly and proportionally to the increase in nitrogen doses in Santa Maria and Frederico Westphalen enviromnents. Therefore, flaxseed responds differently to nitrogen supplementation according to the cultivation enviromnent. showing wide variability. The different doses of nitrogen influenced the variables analyzed and the varieties of flaxseed differently, depending on the enviromnent.

Keywords: Linum usitatissimum L.; fertilizer; multi-environment; nitrogen.

INTRODUCTION

The grains of flaxseed (Linum usitatissimum L.) as they are also known, have been reaching prominence in the world due to the a-linolenic acid (cu-З) content shown in their oil (Madhusudhan, 2009; Lee etai., 2021). Inhumans. cu-З can prevent certain types of cancer, strokes, autoimmune diseases such as systemic lupus erythematosus, cardiovascular diseases (Morris & Vaisey-Genser, 2003), decrease bad cholesterol, diabetes (Ricklefs-Jolmson et al.. 2017; Zhu et al., 2020) and provide an increase in docosahexaenoic acid (DHA) that is directly related to the formation, development, and functioning of the brain and retina (Martin et al., 2006).

The agronomic characteristics an cu-З contents in the oil depend on the flax variety or cultivar (Dmitriev et al., 2020), the cultivation conditions (Morris & Vaisey-Genser, 2003) and especially the fertilization used. Nitrogen fertilizers contribute to a greater extent to the variation in oil content in flax grains and. in addition, to high crop yields. In Brazil, the average grain yield is around 1,000 kg ha1 (IBGE, 2016), while countries such as Sweden, France. and Canada showed averages of 3,918, 1,931, and 1,543 kg ha1 respectively, evidencing the yield potential of the crop (Popovic, 2016). Thus, as in other crops, the flaxseed tends to vary its yield and its phenotypic expression according to the environment in which it is positioned and according to the availability of nutrients in the system, among these nutrients, nitrogen. Considered essential and an important fertility factor, nitrogen increases grain production promoting canopy formation that is necessary for photosynthesis, and consequently increasing crop yields by about 40% (Zörb et al., 2018).

The flaxseed is little explored in research in Brazil, resulting in low use and technical knowledge about the crop, generating a lack of information about proper management, responses to fertilization, and, likewise, the appropriate supply of nitrogen. Studies on nitrogen manuring are carried out in countries such as Egypt (El-Gedwy et al., 2018; Einam, 2019; Abdel-Kader & Mousa 2019; El-Gedwy, 2018), Ethiopia (Taddese & Tenaye, 2018), Kenya (Karinki et al., 2014), Iran (Khajani et al., 2012), Canada (Ibrahim, 2009), China (Shi & Zhao, 2018; Zhang et al., 2020), India (Chopra & Badiyala, 2016; Patel et al., 2017), Russia (Prakhova & Turína, 2021), Iran (Khajani et al., 2012), Japan (Hatanaka et al., 2021) and Poland (Andruszczak et al., 2015).

In the literature Shi&Zhao (2018) and Cui et al. (2019) report that excessive nitrogen application reduces the rate of plant dry biomass accumulation. At the advanced growth stage, it destroys the carbon and nitrogen balance and consequently affects the synthesis of photosynthetic products and their translocation to grain (Yau et al. ,2018), reflecting lower grain weight, often non-viable and low vigor. The content of non-structural carbohydrates shown in various flaxseed plant organs has a negative correlation with nitrogen concentration (Yau et al, 2018), thus reducing the rate of contribution of these non-structural carbohydrates to yield before flowering, which in turn decreased flax grain yield. In addition, the high application of nitrogen can favor lodging, which, in addition to reducing grain yield, makes mechanical harvesting difficult and also increases the cost to the fanner.

Tlris study is based on the lack of studies related to nitrogen fertilizers in the flaxseed crop, as well as the lack of knowledge about appropriate supply under cultivation conditions in Brazil. Hence, the objective was to estimate the appropriate nitrogen dose for the development of the variables technical length, yield length, main stem diameter, number of grains per pod, plant grain yield, and plant dry mass of two flax varieties, brown and golden, in places with subtropical and tropical climate in Brazil.

MATERIAL AND METHODS

The experiment was carried out in a test network in three different enviromnents: Santa Maria (29°43'23"S and 53°43' 15"W, at an altitude of 95m) and Frederico Westphalen (27°39'56"S and 53°42'94"W, at 493 m altitude), in the state of Rio Grande do Sul (RS); and Viçosa (20° 45' 14" S and 42° 52' 55" W, at an altitude of 648 m), in the state of Minas Gerais (MG). The climate, according to the Koppen classification, is classified as Cfa for the municipalities of the state of RS and Cwa for the municipality of MG. (Alvares et al., 2013). The meteorological characterizations of each location were obtained from automatic meteorological stations, located close to the experiments and are represented in Figure 1.

The treatments consisted of a two-factor 2x6 experiment, consisting of two flax varieties (brown and golden), grown by producers in the region of Panambi-RS-Brazil with seeds provided by CISBRA company, and six nitrogen doses (0; 30; 60; 90; 120 and 150 kg ha1) in randomized blocks with four repetitions. The randomization of the treatments followed the structure of split plots, where the flax varieties were randomized in the main plots and the nitrogen doses in the subplots. The experimental units used were plots of 2.5 m in length by 2.0 m in width, equivalent to an area of 5 m2 and seeding was carried out manually in June with density be 80.0 kg of seeds viable per hectare. Each plot consisted of ten lines with a spacing of 20 cm between lines, considering a useful area of eight central lines per plot.

The study lasted 162 days, from June to November 2020. The soils of the different flax cultivation regions are classified as: Arenie Dystrophic Red-Yellow Argisol for the municipality of Santa Maria, Alumino-Ferric Red Latossol for the municipality of Frederico Westphalen-RS, and Red Yellow Argisol for the municipality of Viçosa-MG (Embrapa, 2013). The base manuring was carried out according to the results of the soil analysis of each place and with the application of the NPK 5-20-20 formula, appropriate to the expectation of production of 2 t ha1, according to the manuring and liming, ROLAS (2004) for the crop of flaxseed. The nitrogen application hauling to the crop was performed in a single dose from the beginning of brandling and was provided in the form of urea.

Upon reaching the point of harvest maturation, 20 plants were harvested per plot, which was cut close to the ground in a sequence of five plants, at four random points in the useful area of the plot. Subsequently, the collected plants were taken to the laboratory for analysis of the variables: technical length (cm) obtained by measuring the base of the plant to the region of insertion of the first stems with capsules; yield length (cm) estimated from the calculation of the plant height measurement minus technical length; main stem diameter (mm) measured at 10 cm above the base of the plant; the number of grain per pod; plant grain yield (g) and plant dry mass (g).

After data collection, the residual normality test proposed by Shapiro-Wilk and the variance homogeneity test proposed by Oneil and Matthews were performed. An analysis of variance was carried out for the response variables, and the factors were analyzed by the Tukey test at 0.05 of error probability, except for the nitrogen doses, which were adjusted to polynomial models, to find the ideal nitrogen dose for obtaining the best result, and the expression used MET = -when 2x/?2

the regression model was quadratic and the expression -2 x ß2 ± Jdx ß?2 -12x R x R .

MET =_____ 1 3 for cubic

6x ß3

regression models. All statistical analyzes were performed adopting a 5% error and with the ExpDes and ggplotl packages of the R software (R Development Core Team, 2020).

RESULTS AND DISCUSSIONS

The assumptions of the analysis of variance, homogeneity, and normality of the experimental residues were 100% met and the coefficients of variation (CV%) ranged from 3.20 to 23.40% (Table 1), giving reliability to the results.

In the analysis of variance, the enviromnent of Santa Maria - RS showed significance in the interaction "variety x nitrogen dose" only in the variable plant grain yield. The variables yield length and main stem diameter were significant for the "variety" factor and the "nitrogen dose" factor. In the variables teclmical length and plant dry mass, the significance occurred only for the factor "nitrogen dose", while for the variable number of grains per capsule there was significance only in the "variety" factor.

For the enviromnent of Frederico Westphalen - RS, there was a significant interaction between the factors "variety x nitrogen dose" for the variable teclmical length. The variables main stem diameter, plant dry mass, and plant grain yield showed significance for the factor "nitrogen dose". The variables yield length and number of grains per capsule showed a significant effect only for the "variety" factor.

In Viçosa - MG, there were no significant interactions between the factors "variety x nitrogen dose" for any of the variables studied and, also, it was not significant for the factor "nitrogen dose", showing exclusively the effect of the variety for the variables teclmical length, yield length, number of grains per capsule and plant dry mass.

Comparing the varieties of flaxseeds, brown and golden in the enviromnent of Santa Maria - RS, for the variables main stem diameter, the number of grains per pod, and plant grain yield, it appears that the golden variety obtained values of 2.08 cm, 6.96 and 5.95 g significantly higher compared to the values of the brown variety 1.88 cm, 6.34 and 4.66 g, respectively. As for the yield length variable, the brown variety showed the highest average for this variable (18.17 cm), compared to the golden variety (11.82 cm) (Table 1).

Similarly, in Frederico Westphalen - RS, the brown flaxseed variety showed a significantly higher mean value of yield length (10.50 cm), when compared to the golden variety (8.79 cm). It also showed a significantly higher value of the teclmical length of 58.42 cm compared to the golden variety with 54.12 cm. However, concerning the variable number of grains per pod, the golden variety showed a higher mean value of 8.85, compared to the brown with 7.98, in Frederico Westphalen - RS (Table 1).

In Viçosa - MG, the brown variety showed significantly the highest averages of teclmical length (82.76 cm), yield length (12.46 cm), and plant dry mass (2.58 g). While for the variable number of grains per pod, the result was the opposite, as the golden variety had the highest average of 9.18 grains per pod, compared to the brown variety with 8.87 grains. This superiority in the number of grains per capsule was also verified in the enviromnents of Santa Maria and Frederico Westphalen.

The prominence shown by the brown variety concerning teclmical length and yield length, in the three cultivation enviromnents, may be linked to the genetic variability of flax, which is quite wide (Velho & Lúcio, 2021). Another point to be taken into consideration is the association of technical and yield length with the total height of the plant since both are fractions of the total height. Fila et al. ,(2018), reported that plant height was negatively associated with grain yield. Similarly, with this result found by the authors, in the present study, the variables number of grains per capsule and plant grain yield obtained the lowest averages in the brown variety, compared to the golden variety, in the three enviromnents. Birhanu et al., (2020), evaluating 12 flax genotypes in Southern Tigray upland and water stress, observed a significant positive correlation between the number of grains per capsule and grain yield. From this correlation, it was possible to infer that the golden variety, with significantly higher averages in the number of grains per capsule, was more productive compared to the brown variety, regardless of the enviromnent in which it was cultivated.

In the regression analysis of the interaction "variety x nitrogen doses" in the enviromnent of Santa Maria - RS under the variable plant grain yield, there was a significant quadratic polynomial adjustment for the golden variety, with a point of maximum technical efficiency (MTE) at the dose of 125.14 kg ha1 of nitrogen (Figure 2). The brown variety, on the other hand, did not show significant polynomial model adjustments. In a study with flaxseed in Dingxi, Gansu province in China, Zhang et al. (2020) found that grain yield increased with intermediate doses of nitrogen. Compared to the conventional recommendation for flax crop in China (150 kg ha1 of nitrogen), grain yield under the lower nitrogen doses of 120, 90, 60, and 30 kg ha1 increased by 15.63,18.21, and 16.39% respectively, the yield of flax grains.

For the regression analysis of the factor "nitrogen dose", under the average of the brown and golden varieties, in the Santa Maria - RS enviromnent, the variables yield length (Figure 3a), and plant dry mass (Figure 3d) were adjusted to linear polynomial models, with a respective increment of 78 and 182% in the highest nitrogen dose (150 kg ha1), concerning the control treatment (0 kg ha1). The yield length represents the place where the capsules are inserted, which, in turn, contain the seeds. Thus, it can be a relevant variable for obtaining higher yields. Mohammadi et al. (2022) reported positive and significant associations between grain yield and yield length. El-Yamanee (2011) reported that increasing nitrogen doses induced a significant increase in yield length. Kulpa & Dauert (1962), classified plants with a yield length equivalent to 1/2 of the total plant, as appropriate for the production of large seeds. In addition, the linear increase in plant dry mass is also related to the increase in nitrogen doses and can be attributed to the increases in technical length, yield length, and main stem diameter, which occurred in nitrogen-supplemented flaxseed plants. This growing increase in plant dry mass is also reported by Shi & Zhao (2018) and Cui et al. (2019), where excessive nitrogen application reduced the rate of flax plants dry biomass accumulation.

For the variable technical length (Figure 3c), in the enviromnent of Santa Maria - RS and the "nitrogen dose" factor, there was a quadratic polynomial adjustment, with a point of maximum technical efficiency at the dose of 106.47 kg ha1 of nitrogen. On the other hand, the variable main stem diameter (Figme 3b) obtained a significant cubic polynomial adjustment, with a point of maximum technical efficiency estimated at the dose of 80.92 kg ha1 of nitrogen, but it is observed in the figme that the highest dose of N (150 kg ha1) was the one that provided the highest average main stem diameter. The increase in these variables associated with the increase in nitrogen doses may be related to the action of this macronutrient in the increase of meristematic activity and cell division, causing an increase in the number and also the size of cells in the stem of flaxseed. El-Gedway (2018) reports that flax plants fertilized with 70 kg ha1 of nitrogen obtained greater technical length and main stem diameter. The increase in the main stem diameter can be a very valid characteristic to be measmed, flax plants with a larger diameter are more resistant to lodging caused by abiotic factors, such as wind and rain. Lodging is more frequent in flax than in wheat, for example (Vera et al.. 2012).

In the enviromnent of Frederico Westphalen - RS, it was found that the increase in nitrogen doses provided a linear increase in the technical length of the golden variety of flaxseed plants, with an increase of 16.17% in the highest nitrogen dose (150 kg ha1), concerning non-supplementary plants (0 kg ha1) (Figure 4a). There was also a significant polynomial adjustment for the brown variety, however, the adjustment was quadratic with the point of maximum technical efficiency of the nitrogen doses under the technical length of the flax plants of the brown variety, at the dose of 121.90 kg ha1 of nitrogen (Figure 4b). In studies with flaxseed crops El-Gedwy et al. (2018), Abdel-Kader & Mousa (2019) and Einam (2019) reached similar results where there was an increase in technical plant length with nitrogen supplementation.

Concerning the regression analysis of the "nitrogen doses", still in the enviromnent of Frederico Westphalen - RS. the variables main stem diameter (Figure 5a) and plant grain yield (Figure 5c) showed a significant polynomial and quadratic adjustment, with maximum technical efficiency at the dose of 134.95 and 100.38 kg ha1 of nitrogen, respectively. In contrast, Zhang et al. (2020) in a similar study with flaxseed reported that 90 kg ha1 was the best nitrogen dose to improve flax grain yield, i.e.. much lower doses than those found in the present study. However, similarly, the sharp increase in grain yield associated with the increase in nitrogen doses was also observed by Khajani et al. (2012), Karinki et al. (2014), Andruszczak et al. (2015), Chopra & Badiyala (2016), Patel et al. (2017), Taddese & Tenaye (2018) and Yau et al. (2018). In addition, the increase in the main stem diameter with nitrogen doses was observed by El-Yamanee (2011). El-Gedway et al. (2018), reported that the dose of 70 kg ha1 of nitrogen provided the greatest thickness in the main stem diameter, a value much lower than the dose of 134.95 kg ha1 found in the present study.

In the variable plant dry mass (Figure 5b). in the enviromnent of Frederico Westphalen - RS. the significant polynomial adjustment was linear and as the nitrogen doses increased, increased the flaxseed plants dry mass. It was also possible to observe that the highest dose of nitrogen (150 kg ha1) provided an increase of 34.44% in the plant dry mass, compared to the control treatment (0 kg ha1). Tins linear increase in plant dry mass also occurred in the enviromnent of Santa Maria - RS. In contrast. Zhang et al. (2020) found that the dry mass increased by 36.13% with the application of 90 kg ha1 of nitrogen, a dose much lower than the linear increase found in the present study with the highest doses. The authors also report that the application of doses above 90 kg ha1 of nitrogen reduced the number of total flax plants' dry mass. Shi & Zhao (2018) and Cui et al. (2019) state that excessive nitrogen application reduced the rate of plant dry biomass accumulation.

The increase in plant dry mass due to the application of nitrogen fertilizers can be explained by the effect of nitrogen stimulation on growth attributes, maximizing the use of photosynthesis and. consequently, the number of metabolites, as well as the formation of new stem cells providing. Thus, an increase in technical length, yield length, and main stem diameter. Tins tendency of increasing dry mass with the application of nitrogen fertilizer was also observed by Khajani et al. (2012), Karinki et al. (2014),

Andruszczak et al. (2015), Chopra & Badiyala (2016) and Patel et al. (2017).

In general, it is possible to infer that flaxseed responds differently to nitrogen supplementation according to the cultivation enviromnent. showing wide variability.

CONCLUSION

The brown variety obtained greater technical and yield length compared to the golden variety.

The golden variety showed a higher number of grains per capsule and higher plant grain yield compared to the brown variety, in the three enviromnents (Santa Maria - RS. Frederico Westphalen - RS. and Viçosa - MG).

The plant dry mass increased linearly and proportionally to the increase in nitrogen doses in the enviromnents of Santa Maria - RS and Frederico Westphalen - RS. The different doses of nitrogen applied influenced the variables analyzed and the varieties of flaxseed differently, depending on the enviromnent.

Sidebar

Submitted on January 30th and accepted on February 13th, 2023.

'Corresponding author: [email protected]

References

REFERENCES

Abdel-Kader EMA & Mousa MA (2019) Effect of nitrogen fertilizer on some flax varieties under two different location conditions. Journal of Plant Production, 10:37-44.

Alvares CÁ., Stape JL, Sentelhas PC, Gonçalves JLM & Sparovek G (2013) Köppen's climate classification map for Brazil. Meteorologische Zeitschrift, 22:711-728.

Andruszczak S, Gawlik-Dziki U, Kraska P, Kwiecińska-Poppe E, Różyło К & Palys E (2015) Yield and quality traits of two linseed (Linum usitatissimum L.) cultivars as affected by some agronomic factors. Plant, Soil and Environment, 61:247-252.

Birhanu AG (2020) Yield evaluation and character association of linseed (Linum usitatissimum L.) genotypes in moisture stress areas of South Tigray, Ethiopia. Journal of Cereals and Oilseeds, 11:16-20.

Chopra P & Badiyala D (2016) Influence of nitrogen fertilization on performance of linseed (Linum usitatissimum L.) under utera system. Himachal Journal of Agricultural Research, 42:91-93.

Dmitriev A.A, Novakovskiy RO, Pushkova EN, Rozhmina TA, Zhuchenko AA, Bolsheva NL, Beniaminov AD, Mitkevich VA, Povkhova LV, Dvorianinova EM, Snezhkina AV, Kudryavtseva AV, Krasnov GS & Melnikova NV (2020) Trasncriptomes of different tissues of flax (finim usitatissimum L.) cultivars with diverse characteristics. Frontiers in Genetics, 11:01-06.

El-Gedway EMM, Hammam GYM, Állam SAH, Mostafa SHA & ElShimy KHSS (2018) Effect of irrigation intervals and nitrogen fertilizer rates on flax yield and some anatomical manifestations. Menoufia Journal of Plant Production, 3:01-19.

El-Yamanee GSA (2011) Response of flax plant (Linum usitatissimum L) to treatments with mineral and biofertilizers. Doctoral Thesis. Cairo, Cairo University. 82p.

Ernam S (2019) Cultivars response of flax (Linum usitatissimum L.) to different nitrogen sources in dry environment. Egyptian Journal of Agronomy, 41:119-131.

Embrapa (2013) Sistema Brasileiro de Classificaçao de Solos. 3a ed. Brasilia, Embrapa. 353p.

Fila G, Bagatta M, Maestrini C, Potenza E & Matteo R (2018) Linseed as a dual-purpose crop: evaluation of cultivar suitability and analysis of yield determinants. Journal of Agricultural Science, 156:162-176.

Hatanaka T, Yamamoto N, Araki R, Kishigami M, Nakamoto ?, Masumura T & Sugimoto T (2021) Fatty acid compositions of tri acylglycerols in flax (Linum usitatissimumL.) seeds with varied seeding dates and nitrogen fertilization in a temperate region of Japan. Soil Science and Plant Nutrition, 67:269-276.

IBGE - Instituto Brasileiro de Geografia e Estatistica (2016) Censo Agropecuario. Available at: <https://seriesestatisticas.ibge.gov.br/ series. aspx?no=l &op=0&vcodigo=PA3&t=la%2520vouratemporaria-quantidade-produzida. Acesso%2520em%3a08%2f04%2f2015>. zAccessed on: April 12th, 2022.

Ibrahim HM (2009) Effect of sowing date and N-fertilizer rates on seed yield, some yield components and oil content in flax. ^Alexandria Journal of zAgricultural Sciences, 54:19-28.

Kariuki W, Mas inde PW, Onyango zAN, Githiri SM & Ogila К (2014) The growth and seed yield of five linseed (Linum usitatissimum L.) varieties as influenced by nitrogen application. Journal of zAnimal & Plant Sciences, 22:3493-3509.

Khajani FP, Irannezhad H, Majidian M & Oraki H (2012) Influence of different levels of nitrogen, phosphorus and potassium on yield and yield components of flax seed oil (Linum usitatissimum L.) variety Lirina. Journal of Medicinal Plants Research, 6:1050-1054.

Kulpa W & Danert S (1962) ZurSystematik von: Linum usitatissimum L. Kulturpflanze Beiheft, 3:341-388.

Lee SH, Kim YB, Kim DH, Lee DW, Lee HG, Jha R & Lee KW (2021). Dietary soluble flaxseed oils as a source of omega-3 polyunsaturated fatty acids for laying hens. Poultry Science, 100:101276.

Madhusudhan В (2009) Potential Benefits of Flaxseed in Health and Disease - zA Perspective. zAgriculturae Conspectus Scientificus, 74:6772.

Martin CzA, zAlmeida VV, Ruiz MR, Visentainer JEL, Matshushita M, Souza NE & Visentainer JV (2006) zAcidos graxos poli-insaturados ômega-3 e ômega-б: importancia e ocorrencia em alimentos. Revista de Nutriçao, 19:761-770.

Mohammadi M, Sharifi P, Karimizadeh R & Shefazadeh MK (2012) Relationships between Grain Yield and Yield Components in Bread Wheat under Different Water Availability (Dryland and Supplemental Irrigation Conditions). Notulae Botanicae Horti zAgrobotanici, 40:195200.

Morris DH & Vaisey-Genser M (2003) Flaxseed. In: Benjamin Caballero (Eds.) Encyclopedia of Food Sciences and Nutrition. Baltimore, Johns Hopking University, p.2525-2531.

Patel RK, Tomar GS & Dwivedi SK (2017) Effect of irrigation scheduling and nitrogen levels on growth, yield and water productivity of linseed (Linum usitatissimum L.) under Vertisols. Journal of zApplied and Natural Science, 9:698-705.

Popovic V, Sikora V, Tátié M, Filipović V, Terzić D, Janjić S & Brdar-Jokanović M (2016) zAnalysis of production of linseed (Linum usitatissimumŁ.) in the world. In: 20th International Eco-Conference and 9th Intemationational Eco-Conferenceon Safe Food, Novi Sadi. Proceedings, IUNS. p. 119-127.

Prakhova TYA & Turiną EL (2021) Effect of mineral fertilizers on productivity and quality of winter false flax. IOP Conference Series: Earth and Environmental Science, 937:01-06.

R Development Core Team (2020) R: zA Language and environment for statistical computing. Available at: <https://www.r-project.org/>. zAccessed on: March lrst, 2020.

Ricklefs-Johnson K, Johnston CS & Sweazea KL (2017) Ground flaxseed increased nitric oxide levels in adults with type 2 diabetes: zA randomized comparative effectiveness study of supplemental flaxseed and psyllium fiber. Obesity Medicine, 5:16-24.

ROLzAS - Rede Oficial de Laboratorios de zAnálise de Solo e de Tecido Vegetal (2004) Manual de adubaçao e calagem para os estados do Rio Grande do Sul e Santa Catarina. 10a ed. Porto zAlegre, Sociedade Brasileira de Ciencia do Solo. 400p.

Shi HJ & Zhao YW (2018) Effects of different nitrogen application rates on growth and yield of upland flax. zAgricultural Science of Technological Information, 20:40-42.

Taddese G & Tenaye S (2018) Effect of nitrogen on flax (Linum usitatissimum L.) fiber yield at debreberhan area, Ethiopia. Forestry Research and Engineering: International Journal, 2:284-286.

Velho JP & Lúcio zAD (2021) Linhaça: perspectiva de produçao e usos na alimentaçao humana e animal. Ponta Grossa, zAtena Editora. 156p.

Vera CL, Duguid SD, Fox SL, Rashid KY, Dribnenki JCP & Clarke FR (2012) Comparative effect of lodging on seed yield of flax and wheat. Canadian Journal of Plant Science, 92:39-43.

Yan B, Bing WU, Gao Y & Jianmin WU (2018) Effects of nitrogen and phosphorus dosage on accumulation, transport and yield of non-structural carbohydrate in flax. Chinese Soil Fertility, 2:63-69.

Zhang Q, Gao Y, Yan B, Cui Z, Wu B, Yang К & Ma J (2020) Perspective on oil flax yield and dry biomass with reduced nitrogen supply. Oil Crop Science, 5:42-46.

Zhu L, Sha L, Li K, Wang Z, Wang T, Li Y, Liu P, Dong X, Dong Y, Zhang X & Wang H (2020) Dietary flaxseed oil rich in omega-3 suppresses severity of type 2 diabetes mellitus via anti-inflammation and modulating gut microbiota in rats. Lipids in Health and Disease, 19:01-16.

Zörb C, Ludwing U & Hawkesford MJ (2018) Perspective on wheat yield and quality with reduced nitrogen supply. Trends in Plant Science, 23:1029-1037.