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© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

The genome composition of intermediate wheatgrass (IWG) is complex and continues to be a subject of investigation. In this study, molecular cytogenetics were used to investigate the karyotype composition of Th. intermedium and its relative diploid species. St2-80 developed from Pseudowroegneria strigose and pDb12H developed from Dasypyrum breviaristatum were used as probes in fluorescence in situ hybridization (FISH) to classify the chromosomes of Th. intermedium into three groups, expressed as JvsJvsJrJrStSt. A combined multiplex oligonucleotide probe, including pSc119.2-1, (GAA)10, AFA-3, AFA-4, pAs1-1, Pas1-3, pAs1-4, and pAs1-6, was used to establish the FISH karyotype of ten accessions of Th. intermedium. Variability among and within the studied accessions of intermediate wheatgrass was observed in their FISH patterns. Results of this study led to the conclusions that Jvs had largely been contributed from Da. breviaristatum, but not the present-day Da. villosum; IWG had only one J genome, Jr, which was related to either Th. elongatum or Th. bessarabicum; and St was contributed from the genus Pseudoroegneria by hybridization with Th. junceiforme or Th. sartorii.

Details

Title
Genome Analysis of Thinopyrum intermedium and Its Potential Progenitor Species Using Oligo-FISH
Author
Qi, Fei 1 ; Liang, Shuang 2 ; Xing, Piyi 3 ; Bao, Yinguang 3 ; Wang, Richard R-C 4   VIAFID ORCID Logo  ; Li, Xingfeng 3 

 State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, China; [email protected] (F.Q.); [email protected] (P.X.); [email protected] (Y.B.) 
 Agronomy College, Shandong Agricultural University, Tai’an 271018, China 
 State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, China; [email protected] (F.Q.); [email protected] (P.X.); [email protected] (Y.B.); Agronomy College, Shandong Agricultural University, Tai’an 271018, China 
 USDA-ARS Forage & Range Research Laboratory (FRRL), Logan, UT 84322-6300, USA 
First page
3705
Publication year
2023
Publication date
2023
Publisher
MDPI AG
e-ISSN
22237747
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2888351518
Copyright
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.