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© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

The application of synthetic iron chelates to overcome iron deficiency in crops is leading to a high impact on the environment, making it necessary to find more friendly fertilizers. A promising alternative is the application of biodegradable iron chelates, such as those based on siderophores. In the present work, seven bacterial strains of the genus Pseudomonas were selected for their ability to secrete pyoverdine, a siderophore with a high affinity for iron, which could be used as a biofertilizer. The concentration of siderophores secreted by each bacterium expressed as desferrioxamine B equivalents, and the pyoverdine concentration was determined. Their potential as Fe biofertilizers was determined based on their capacity to complex Fe, determining the maximum iron complexation capacity at alkaline pH and selecting the RMC4 strain. The biostimulant capacity of the RMC4 strain was evaluated through the secretion of organic acids such as the hormone Indol-3-acetic acid or glutamic acid, among others, in a kinetic assay. Finally, the genome of RMC4 was determined, and the strain was identified as Pseudomonas monsensis. The annotated genome was screened for genes and gene clusters implicated in biofertilization and plant growth promotion. Besides iron mobilization, genes related to phosphorus solubilization, production of phytohormones and biological control, among others, were observed, indicating the suitability of RMC4 as an inoculant. In conclusion, RMC4 and its siderophores are promising sources for Fe biofertilization in agriculture.

Details

Title
Evaluation of Siderophores Generated by Pseudomonas Bacteria and Their Possible Application as Fe Biofertilizers
Author
Lozano-González, José María 1 ; Valverde, Silvia 1   VIAFID ORCID Logo  ; Montoya, Mónica 2   VIAFID ORCID Logo  ; Martín, Marta 3   VIAFID ORCID Logo  ; Rivilla, Rafael 3   VIAFID ORCID Logo  ; Lucena, Juan J 1   VIAFID ORCID Logo  ; López-Rayo, Sandra 1   VIAFID ORCID Logo 

 Department of Agricultural Chemistry and Food Science, Universidad Autónoma de Madrid, Av. Francisco Tomás y Valiente 7, 28049 Madrid, Spain; [email protected] (J.M.L.-G.); [email protected] (S.V.); [email protected] (J.J.L.) 
 Department of Biology, Universidad Autónoma de Madrid, c/Darwin, 2, 28049 Madrid, Spain; [email protected] (M.M.); [email protected] (M.M.); [email protected] (R.R.); Departamento de Química y Tecnología de Alimentos, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de Madrid, Ciudad Universitaria, 28040 Madrid, Spain 
 Department of Biology, Universidad Autónoma de Madrid, c/Darwin, 2, 28049 Madrid, Spain; [email protected] (M.M.); [email protected] (M.M.); [email protected] (R.R.) 
First page
4054
Publication year
2023
Publication date
2023
Publisher
MDPI AG
e-ISSN
22237747
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2899411546
Copyright
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.