Abstract

Posterolateral spinal fusion (PLF) is a procedure used for the treatment of degenerative spine disease. In this study we evaluated Osteogrow-C, a novel osteoinductive device comprised of recombinant human Bone morphogenetic protein 6 (rhBMP6) dispersed in autologous blood coagulum with synthetic ceramic particles, in the sheep PLF model. Osteogrow-C implants containing 74–420 or 1000–1700 µm ceramic particles (TCP/HA 80/20) were implanted between L4–L5 transverse processes in sheep (Ovis Aries, Merinolaandschaf breed). In the first experiment (n = 9 sheep; rhBMP6 dose 800 µg) the follow-up period was 27 weeks while in the second experiment (n = 12 sheep; rhBMP6 dose 500 µg) spinal fusion was assessed by in vivo CT after 9 weeks and at the end of the experiment after 14 (n = 6 sheep) and 40 (n = 6 sheep) weeks. Methods of evaluation included microCT, histological analyses and biomechanical testing. Osteogrow-C implants containing both 74–420 and 1000–1700 µm ceramic particles induced radiographic solid fusion 9 weeks following implantation. Ex-vivo microCT and histological analyses revealed complete osseointegration of newly formed bone with adjacent transverse processes. Biomechanical testing confirmed that fusion between transverse processes was complete and successful. Osteogrow-C implants induced spinal fusion in sheep PLF model and therefore represent a novel therapeutic solution for patients with degenerative disc disease.

Details

Title
Calcium phosphate ceramics combined with rhBMP6 within autologous blood coagulum promote posterolateral lumbar fusion in sheep
Author
Ivanjko, Natalia 1 ; Stokovic, Nikola 1 ; Pecin, Marko 2 ; Vnuk, Drazen 2 ; Smajlovic, Ana 2 ; Ivkic, Niko 2 ; Capak, Hrvoje 3 ; Javor, Ana 3 ; Vrbanac, Zoran 3 ; Maticic, Drazen 2 ; Vukicevic, Slobodan 1   VIAFID ORCID Logo 

 University of Zagreb, Laboratory for Mineralized Tissues, Center for Translational and Clinical Research, School of Medicine, Zagreb, Croatia (GRID:grid.4808.4) (ISNI:0000 0001 0657 4636); University of Zagreb, Center of Excellence for Reproductive and Regenerative Medicine, School of Medicine, Zagreb, Croatia (GRID:grid.4808.4) (ISNI:0000 0001 0657 4636) 
 University of Zagreb, Clinics for Surgery, Orthopedics and Ophthalmology, Faculty of Veterinary Medicine, Zagreb, Croatia (GRID:grid.4808.4) (ISNI:0000 0001 0657 4636) 
 University of Zagreb, Department of Radiology, Ultrasound Diagnostics and Physical Therapy, Faculty of Veterinary Medicine, Zagreb, Croatia (GRID:grid.4808.4) (ISNI:0000 0001 0657 4636) 
Pages
22079
Publication year
2023
Publication date
2023
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2900960768
Copyright
© The Author(s) 2023. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.