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© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Migratory flows and international travel are triggering an increase in imported cases of schistosomiasis in non-endemic countries. The present study aims to evaluate the effectiveness of the LAMP technique on patients’ urine samples for the diagnosis of imported schistosomiasis in a non-endemic area in comparison to a commercial immunochromatographic test and microscopic examination of feces and urine. A prospective observational study was conducted in sub-Saharan migrants attending the Tropical Medicine Unit, Almería, Spain. For schistosomiasis diagnosis, serum samples were tested using an immunochromatographic test (Schistosoma ICT IgG-IgM). Stool and urine samples were examined by microcopy. Urine samples were evaluated by combining three LAMP assays for the specific detection of Schistosoma mansoni, S. haematobium, and for the genus Schistosoma. To evaluate the diagnostic accuracy, a latent class analysis (LCA) was performed. In total, 115 patients were included (92.2% male; median age: 28.3 years). Of these, 21 patients (18.3%) were diagnosed with schistosomiasis confirmed by microscopy, with S. haematobium being the most frequent species identified (18/115; 15.7%). The Schistosoma ICT IgG-IgM test result was 100% positive and Schistosoma-LAMP was 61.9% positive, reaching as high as 72.2% for S. haematobium. The sensitivity and specificity estimated by LCA, respectively, were: 92% and 76% for Schistosoma ICT IgG-IgM, 68% and 44% for Schistosoma-LAMP, and 46% and 97% for microscopy. In conclusion, the Schistosoma-LAMP technique presented a higher sensitivity than microscopy for the diagnosis of imported urinary schistosomiasis, which could improve the diagnosis of active infection, both in referral centers and in centers with limited experience or scarce resources and infrastructure.

Details

Title
Evaluation of Loop-Mediated Isothermal Amplification (LAMP) in Urine Samples for the Diagnosis of Imported Schistosomiasis
Author
Salas-Coronas, Joaquín 1   VIAFID ORCID Logo  ; Luzón-García, María Pilar 2   VIAFID ORCID Logo  ; Crego-Vicente, Beatriz 3   VIAFID ORCID Logo  ; Manuel Jesús Soriano-Pérez 2   VIAFID ORCID Logo  ; Febrer-Sendra, Begoña 3   VIAFID ORCID Logo  ; Vázquez-Villegas, José 4   VIAFID ORCID Logo  ; Juan García-Bernalt Diego 3   VIAFID ORCID Logo  ; Cabeza-Barrera, Isabel María 2 ; Castillo-Fernández, Nerea 2 ; Muro, Antonio 3   VIAFID ORCID Logo  ; Bargues, María Dolores 5   VIAFID ORCID Logo  ; Fernández-Soto, Pedro 3   VIAFID ORCID Logo 

 Tropical Medicine Unit, Hospital Universitario Poniente, 04700 El Ejido, Almería, Spain; [email protected] (M.P.L.-G.); [email protected] (M.J.S.-P.); [email protected] (I.M.C.-B.); [email protected] (N.C.-F.); Department of Nursing, Physiotherapy and Medicine, Faculty of Health Sciences, University of Almería, 04120 La Cañada, Almería, Spain 
 Tropical Medicine Unit, Hospital Universitario Poniente, 04700 El Ejido, Almería, Spain; [email protected] (M.P.L.-G.); [email protected] (M.J.S.-P.); [email protected] (I.M.C.-B.); [email protected] (N.C.-F.) 
 Infectious and Tropical Diseases Research Group (e-INTRO), Biomedical Research Institute of Salamanca-Research Centre for Tropical Diseases at the University of Salamanca (IBSAL-CIETUS), Faculty of Pharmacy, University of Salamanca, 37007 Salamanca, Spain; [email protected] (B.C.-V.); [email protected] (B.F.-S.); [email protected] (J.G.-B.D.); [email protected] (A.M.) 
 Tropical Medicine Unit, Distrito Poniente de Almería, 04700 El Ejido, Almería, Spain; [email protected] 
 Department of Parasitology, Faculty of Pharmacy, University of Valencia, 46100 Burjassot, Valencia, Spain; [email protected]; CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III, 28029 Madrid, Spain 
First page
518
Publication year
2023
Publication date
2023
Publisher
MDPI AG
e-ISSN
24146366
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2904917904
Copyright
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.