Abstract

Rodents of the genus Cerradomys belong to tribe Oryzomyini, one of the most diverse and speciose groups in Sigmodontinae (Rodentia, Cricetidae). The speciation process in Cerradomys is associated with chromosomal rearrangements and biogeographic dynamics in South America during the Pleistocene era. As the morphological, molecular and karyotypic aspects of Myomorpha rodents do not evolve at the same rate, we strategically employed karyotypic characters for the construction of chromosomal phylogeny to investigate whether phylogenetic relationships using chromosomal data corroborate the radiation of Cerradomys taxa recovered by molecular phylogeny. Comparative chromosome painting using Hylaeamys megacephalus (HME) whole chromosome probes in C. langguthi (CLA), Cerradomys scotii (CSC), C. subflavus (CSU) and C. vivoi (CVI) shows that karyotypic variability is due to 16 fusion events, 2 fission events, 10 pericentric inversions and 1 centromeric repositioning, plus amplification of constitutive heterochromatin in the short arms of the X chromosomes of CSC and CLA. The chromosomal phylogeny obtained by Maximum Parsimony analysis retrieved Cerradomys as a monophyletic group with 97% support (bootstrap), with CSC as the sister to the other species, followed by a ramification into two clades (69% of branch support), the first comprising CLA and the other branch including CVI and CSU. We integrated the chromosome painting analysis of Eumuroida rodents investigated by HME and Mus musculus (MMU) probes and identified several syntenic blocks shared among representatives of Cricetidae and Muridae. The Cerradomys genus underwent an extensive karyotypic evolutionary process, with multiple rearrangements that shaped extant karyotypes. The chromosomal phylogeny corroborates the phylogenetic relationships proposed by molecular analysis and indicates that karyotypic diversity is associated with species radiation. Three syntenic blocks were identified as part of the ancestral Eumuroida karyotype (AEK): MMU 7/19 (AEK 1), MMU 14 (AEK 10) and MMU 12 (AEK 11). Besides, MMU 5/10 (HME 18/2/24) and MMU 8/13 (HME 22/5/11) should be considered as signatures for Cricetidae, while MMU 5/9/14, 5/7/19, 5 and 8/17 for Sigmodontinae.

Details

Title
Chromosomal rearrangements played an important role in the speciation of rice rats of genus Cerradomys (Rodentia, Sigmodontinae, Oryzomyini)
Author
Oliveira da Silva, Willam 1   VIAFID ORCID Logo  ; Malcher, Stella Miranda 1 ; Ferguson-Smith, Malcolm Andrew 2   VIAFID ORCID Logo  ; O’Brien, Patricia Caroline Mary 2 ; Rossi, Rogério Vieira 3   VIAFID ORCID Logo  ; Geise, Lena 4   VIAFID ORCID Logo  ; Pieczarka, Julio Cesar 1   VIAFID ORCID Logo  ; Nagamachi, Cleusa Yoshiko 1   VIAFID ORCID Logo 

 Universidade Federal do Pará (UFPA), Laboratório de Citogenética, Centro de Estudos Avançados da Biodiversidade, Instituto de Ciências Biológicas, Belém, Brazil (GRID:grid.271300.7) (ISNI:0000 0001 2171 5249) 
 University of Cambridge, Cambridge Resource Centre for Comparative Genomics, Department of Veterinary Medicine, Cambridge, UK (GRID:grid.5335.0) (ISNI:0000 0001 2188 5934) 
 Universidade Federal do Mato Grosso (UFMT), Departamento de Biologia e Zoologia, Instituto de Biociências, Mato Grosso, Brazil (GRID:grid.411206.0) (ISNI:0000 0001 2322 4953) 
 Universidade do Estado do Rio de Janeiro, Departamento de Zoologia, Laboratório de Mastozoologia, Rio de Janeiro, Brazil (GRID:grid.412211.5) (ISNI:0000 0004 4687 5267) 
Pages
545
Publication year
2024
Publication date
2024
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2910058813
Copyright
© The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.