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Abstract
Translational research for the evaluation of physical activity habits and lifestyle modifications based on nutrition and exercise has recently gained attention. In this study, we evaluated the effects of serum samples obtained before and after a 12-week home-based lifestyle intervention based on nutrition and exercise in breast cancer survivors in terms of modulation of the tumorigenic potential of breast cancer cells. The home-based lifestyle intervention proposed in this work consisted of educational counselling on exercise and nutritional behaviors and in 12 weeks of structured home-based exercise. Triple-negative breast cancer cell line MDA-MB-231 was cultured in semi-solid medium (3D culture) with sera collected before (PRE) and after (POST) the lifestyle intervention program. Spheroid formation was evaluated by counting cell colonies after 3 weeks of incubation. Results show a slight but significant reduction of spheroid formation induced by serum collected POST in comparison to those obtained PRE. Moreover, statistical analyses aimed to find physiologic and metabolic parameters associated with 3D cell proliferation revealed the proliferative inducer IGF-1 as the only predictor of cell tumorigenic potential. These results highlight the importance of lifestyle changes for cancer progression control in a tertiary prevention context. Translational research could offer a useful tool to identify metabolic and physiological changes induced by exercise and nutritional behaviors associated with cancer progression and recurrence risk.
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Details
1 University of Urbino Carlo Bo, Unit of Pharmacology and Hygiene, Department of Biomolecular Sciences, Urbino, Italy (GRID:grid.12711.34) (ISNI:0000 0001 2369 7670)
2 University of Urbino Carlo Bo, Division of Exercise and Health Sciences, Department of Biomolecular Sciences, Urbino, Italy (GRID:grid.12711.34) (ISNI:0000 0001 2369 7670)
3 Hospital Santa Maria della Misericordia di Urbino, Medical Oncology, Urbino, Italy (GRID:grid.12711.34)