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Abstract
The Tambaqui is one of the most representative Amazon fish species, being highly exploited in fisheries, aquaculture and as a research model. Nonetheless, data about functional genome are still required to evaluate reproductive and nutrition parameters as well as resistance to pathogens. The of next-generation sequencing has allows assessing the transcriptional processes in non-model species by providing comprehensive gene collections to be used as a database in further genomic applications and increased performance of captive populations. In this study, we relied on RNAseq approach to generate the first transcriptome of the telencephalon from adult males and females of Colossoma macropomum, resulting in a reference dataset for future functional studies. We retrieved 896,238 transcripts, including the identification of 267,785 contigs and 203,790 genes. From this total, 91 transcripts were differentially expressed, being 63 and 28 of them positively regulated for females and males, respectively. The functional annotation resulted in a library of 40 candidate genes for females and 20 for males. The functional enrichment classes comprised reproductive processes (GO:0,048,609; GO:0,003,006; GO:0,044,703; GO:0,032,504; GO:0,019,953) being related to sex differentiation (e.g., SAFB) and immune response (e.g., SLC2A6, AHNAK, NLRC3, NLRP3 and IgC MHC I alpha3), thus indicating that the genes in the neurotranscriptome of Tambaqui participate in sex differentiation and homeostasis of captive specimens. These data are useful to design the selection of genes related to sex determination and animal welfare in raising systems of Tambaqui.
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1 Universidade Federal Do Pará, Laboratório de Genética Aplicada, Instituto de Estudos Costeiros, Bragança, Brazil (GRID:grid.271300.7) (ISNI:0000 0001 2171 5249)
2 Universidade Federal do Oeste do Pará, Monte Alegre, Brazil (GRID:grid.448725.8) (ISNI:0000 0004 0509 0076)
3 Instituto Nacional de Câncer José de Alencar Gomes da Silva (INCA), Divisão de Genética, Rio de Janeiro, Brazil (GRID:grid.419166.d)
4 Ciência E Tecnologia Do Pará, - Campus Bragança, Laboratório de Biologia Molecular e Neuroecologia, Instituto Federal de Educação, Bragança, Brazil (GRID:grid.419166.d)
5 Universidade Federal do Pará, Laboratório de Evolução, Instituto de Estudos Costeiros, Bragança, Brazil (GRID:grid.271300.7) (ISNI:0000 0001 2171 5249)
6 Ciência E Tecnologia Do Pará, - Campus Bragança, Laboratório de Biologia Molecular e Neuroecologia, Instituto Federal de Educação, Bragança, Brazil (GRID:grid.271300.7)
7 Universidade Federal Do Pará, Laboratório de Genética e Biologia Molecular, Instituto de Estudos Costeiros, Bragança, Brazil (GRID:grid.271300.7) (ISNI:0000 0001 2171 5249)