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© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Sarcopenia, a complex and debilitating condition characterized by progressive deterioration of skeletal muscle, is the primary cause of age-associated disability and significantly impacts healthspan in elderly patients. Despite its prevalence among the aging population, the underlying molecular mechanisms are still under investigation. The NLRP3 inflammasome is crucial in the innate immune response and has a significant impact on diseases related to inflammation and aging. Here, we investigated the expression of the NLRP3 inflammasome pathway and pro-inflammatory cytokines in skeletal muscle and peripheral blood of dependent and independent patients who underwent hip surgery. Patients were categorized into independent and dependent individuals based on their Barthel Index. The expression of NLRP3 inflammasome components was significantly upregulated in sarcopenic muscle from dependent patients, accompanied by higher levels of Caspase-1, IL-1β and IL-6. Among older dependent individuals with sarcopenia, there was a significant increase in the MYH3/MYH2 ratio, indicating a transcriptional shift in expression from mature to developmental myosin isoforms. Creatine kinase levels and senescence markers were also higher in dependent patients, altogether resembling dystrophic diseases and indicating muscle degeneration. In summary, we present evidence for the involvement of the NLRP3/ASC/NEK7/Caspase-1 inflammasome pathway with activation of pro-inflammatory SASP in the outcome of sarcopenia in the elderly.

Details

Title
NLRP3 Contributes to Sarcopenia Associated to Dependency Recapitulating Inflammatory-Associated Muscle Degeneration
Author
Antuña, Eduardo 1   VIAFID ORCID Logo  ; Potes, Yaiza 1   VIAFID ORCID Logo  ; Baena-Huerta, Francisco Javier 2 ; Cachán-Vega, Cristina 1   VIAFID ORCID Logo  ; Menéndez-Coto, Nerea 2   VIAFID ORCID Logo  ; Eva Álvarez Darriba 3 ; Fernández-Fernández, Marta 3 ; Natalie Burgos Bencosme 3 ; Bermúdez, Manuel 4 ; López Álvarez, Eva María 4 ; Gutiérrez-Rodríguez, José 4 ; Boga, José Antonio 5 ; Caballero, Beatriz 1   VIAFID ORCID Logo  ; Vega-Naredo, Ignacio 1 ; Coto-Montes, Ana 1   VIAFID ORCID Logo  ; Garcia-Gonzalez, Claudia 1   VIAFID ORCID Logo 

 Research Group OSKAR, Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), 33011 Oviedo, Spain; Department of Morphology and Cell Biology, University of Oviedo, 33006 Oviedo, Spain; Instituto de Neurociencias del Principado de Asturias (INEUROPA), 33006 Oviedo, Spain 
 Department of Morphology and Cell Biology, University of Oviedo, 33006 Oviedo, Spain 
 Geriatric Service, Monte Naranco Hospital, 33012 Oviedo, Spain 
 Research Group OSKAR, Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), 33011 Oviedo, Spain; Geriatric Service, Monte Naranco Hospital, 33012 Oviedo, Spain 
 Grupo de Investigación Microbiología Traslacional, Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), 33011 Oviedo, Spain; Servicio de Microbiología, Hospital Universitario Central de Asturias (HUCA), 33011 Oviedo, Spain 
First page
1439
Publication year
2024
Publication date
2024
Publisher
MDPI AG
ISSN
16616596
e-ISSN
14220067
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2923960475
Copyright
© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.