Nanoengineered chitosan functionalized titanium dioxide biohybrids for bacterial infections and cancer therapy

Abstract

Nanoengineered chitosan functionalized titanium dioxide biohybrids (CTiO₂@NPs) were prepared with Amomum subulatum Roxb extract via one-pot green method and assessed by UV–Vis spectroscopy, XRD, SEM and EDAX analyses. As revealed by XRD pattern, the nanohybrids exhibits a rutile TiO₂ crystallites around 45 nm in size. The emergence of the Ti–O–Ti bond is identified by observing a peak between 400 and 800 cm⁻¹. A wide bandgap (4.8 eV) has been observed in CTiO₂@NPs, due to the quantum confinement effects and the oxygen vacancies reveal the intriguing potential of developed nanohybrids for various applications. Surface flaws were identified by observing an emission band at 382, 437, 482, 517, and 556 nm. They also exhibit better antibacterial performances using well diffusion method against Staphylococcus aureus, Bacillus substilis, Klebsiella pneumonia, and Escherichia coli. CTiO₂@NPs were discovered to have free radical scavenging activity on DPPH analysis and exhibit IC₅₀ value as 95.80 μg/mL and standard (Vitamin C) IC₅₀ is 87.62 μg/mL. CTiO₂@NPs exhibited better anticancer properties against the osteosarcoma (MG-63) cell line. All these findings suggest that there is a forum for further useful therapeutic applications. Therefore, we claim that nano-engineered carbohydrated TiO₂ phytohybrid is a promising solution for bacterial infections and bone cancer.