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© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Control of canine infections with Leishmania infantum (L. infantum), a major zoonotic disease in Brazil and southern Europe, is becoming increasingly important due to its close proximity to humans, the increasing import of dogs from endemic regions and the impact of climate change on vector spreading. Simple, rapid and reliable diagnostic tests are therefore needed to detect infected dogs. Here, we re-evaluated different serological methods for the diagnosis of canine leishmaniosis (CanL) in Croatia and Brazil. The diagnostic performance of the indirect fluorescent antibody test (IFAT) and the VetLine® Leishmania ELISA (GSD Frankfurt, Germany) was compared with three rKLi8.3-based diagnostic test systems, the rKLi8.3 ELISA (GSD Frankfurt, Germany), the INgezim® Leishma CROM (GSD Madrid, Spain) lateral flow test (LFT) and the VetBlot® Leishmania LineBlot (GSD Frankfurt, Germany). CanL symptomatic dogs were efficiently diagnosed by all tests, except the VetLine® Leishmania ELISA, which is based on whole Leishmania antigens. The advantage of rKLi8.3 was also observed in oligo- and asymptomatic dogs from Brazil and Croatia, although with reduced diagnostic efficiency compared to symptomatic dogs. Similar to IFAT and rKLi8.3 ELISA, the LFT did not cross-react with other common canine pathogens; it showed very high specificity for healthy dogs from endemic regions in both countries and did not react with healthy, vaccinated dogs in Brazil. In conclusion, serodiagnostic tests based on the rKLi8.3 antigens are superior to whole parasite antigens, and the LFT has the advantage of providing a laboratory-independent, rapid and specific diagnosis of CanL.

Details

Title
Comparative Study of a Novel Lateral Flow Rapid Test with Conventional Serological Test Systems for the Diagnosis of Canine Leishmaniosis in Croatia and Brazil
Author
Mahdavi, Rouzbeh 1 ; Martinkovic, Franjo 2 ; Shams-Eldin, Hosam 1 ; Pereira, Ingrid E 3 ; Reis, Alexandre B 4   VIAFID ORCID Logo  ; Latz, Andreas 5 ; Heinz, Daniela 5 ; Aira, Cristina 6 ; Fresco-Taboada, Alba 6   VIAFID ORCID Logo  ; Elfadil Abass 7   VIAFID ORCID Logo  ; Romero-Olmedo, Jelena 1 ; Teixeira, Henrique C 3   VIAFID ORCID Logo  ; Steinhoff, Ulrich 1   VIAFID ORCID Logo 

 Institute of Medical Microbiology and Hospital Hygiene, Phillips University of Marburg, 35043 Marburg, Germany; [email protected] (R.M.); [email protected] (H.S.-E.); [email protected] (J.R.-O.) 
 Faculty of Veterinary Medicine, University of Zagreb, HR-1000 Zagreb, Croatia; [email protected] 
 Department of Parasitology, Microbiology and Immunology, Federal University of Juiz de Fora, Juiz de Fora 36036-900, MG, Brazil[email protected] (H.C.T.) 
 School of Pharmacy, Federal University of Ouro Preto, Ouro Preto 35400-000, MG, Brazil; [email protected] 
 Gold Standard Diagnostics Frankfurt (GSD Frankfurt), 63128 Dietzenbach, Germany; [email protected] (A.L.); [email protected] (D.H.) 
 Gold Standard Diagnostics Madrid S.A. (GSD Madrid), 28037 Madrid, Spain; [email protected] (C.A.); [email protected] (A.F.-T.) 
 Department of Clinical Laboratory Science, College of Applied Medical Sciences, Imam Abdulrahman Bin Faisal University, Dammam 34212, Saudi Arabia; [email protected] 
First page
109
Publication year
2024
Publication date
2024
Publisher
MDPI AG
e-ISSN
20760817
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2931029542
Copyright
© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.