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© 2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Background and Aim

Until the May 2022 Monkeypox (MPXV) outbreak, which spread rapidly to many non-endemic countries, the virus was considered a viral zoonosis limited to some African countries. The Andalusian circuit of genomic surveillance was rapidly applied to characterize the MPXV outbreak in the South of Spain.

Methods

Whole genome sequencing was used to obtain the genomic profiles of samples collected across the south of Spain, representative of all the provinces of Andalusia. Phylogenetic analysis was used to study the relationship of the isolates and the available sequences of the 2022 outbreak.

Results

Whole genome sequencing of a total of 160 MPXV viruses from the different provinces that reported cases were obtained. Interestingly, we report the sequences of MPXV viruses obtained from two patients who died. While one of the isolates bore no noteworthy mutations that explain a potential heightened virulence, in another patient the second consecutive genome sequence, performed after the administration of tecovirimat, uncovered a mutation within the A0A7H0DN30 gene, known to be a prime target for tecovirimat in its Vaccinia counterpart. In general, a low number of mutations were observed in the sequences reported, which were very similar to the reference of the 2022 outbreak (OX044336), as expected from a DNA virus. The samples likely correspond to several introductions of the circulating MPXV viruses from the last outbreak. The virus sequenced from one of the two patients that died presented a mutation in a gene that bears potential connections to drug resistance. This mutation was absent in the initial sequencing before treatment.

Details

Title
Molecular and phylogenetic characterization of the monkeypox outbreak in the South of Spain
Author
Casimiro-Soriguer, Carlos S 1 ; Perez-Florido, Javier 1 ; Lara, Maria 2 ; Camacho-Martinez, Pedro 3 ; Merino-Diaz, Laura 3 ; Pupo-Ledo, Inmaculada 3 ; de Salazar, Adolfo 4 ; Fuentes, Ana 5 ; Viñuela, Laura 6 ; Chueca, Natalia 5 ; Martinez-Martinez, Luis 7 ; Lorusso, Nicola 8 ; Lepe, Jose A 9 ; Dopazo, Joaquín 10   VIAFID ORCID Logo  ; Garcia, Federico 4 

 Computational Medicine Platform, Andalusian Public Foundation Progress and Health-FPS, Sevilla, Spain; Institute of Biomedicine of Seville, IBiS, University Hospital Virgen del Rocío/CSIC/University of Seville, Seville, Spain 
 Computational Medicine Platform, Andalusian Public Foundation Progress and Health-FPS, Sevilla, Spain 
 Servicio de Microbiología, Unidad Clínica Enfermedades Infecciosas, Microbiología y Medicina Preventiva, Hospital Universitario Virgen del Rocío, Sevilla, Spain 
 Centro de Investigación Biomédica en Red en Enfermedades Infecciosas (CIBERINFEC), ISCIII, Madrid, Spain; Servicio de Microbiología, Hospital Universitario San Cecilio, Granada, Spain; Instituto de Investigación Biosanitaria, ibs.GRANADA, Granada, Spain 
 Centro de Investigación Biomédica en Red en Enfermedades Infecciosas (CIBERINFEC), ISCIII, Madrid, Spain; Instituto de Investigación Biosanitaria, ibs.GRANADA, Granada, Spain 
 Centro de Investigación Biomédica en Red en Enfermedades Infecciosas (CIBERINFEC), ISCIII, Madrid, Spain; Servicio de Microbiología, Hospital Universitario San Cecilio, Granada, Spain 
 Centro de Investigación Biomédica en Red en Enfermedades Infecciosas (CIBERINFEC), ISCIII, Madrid, Spain; Unit of Microbiology, University Hospital Reina Sofia, Cordoba, Spain; Departamento de Química Agrícola, Edafología y Microbiología, Universidad de Córdoba, Córdoba, Spain; Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Córdoba, Spain 
 Dirección General de Salud Pública, Consejería de Salud y Familias, Junta de Andalucía, Sevilla, Spain 
 Institute of Biomedicine of Seville, IBiS, University Hospital Virgen del Rocío/CSIC/University of Seville, Seville, Spain; Servicio de Microbiología, Unidad Clínica Enfermedades Infecciosas, Microbiología y Medicina Preventiva, Hospital Universitario Virgen del Rocío, Sevilla, Spain; Centro de Investigación Biomédica en Red en Enfermedades Infecciosas (CIBERINFEC), ISCIII, Madrid, Spain 
10  Computational Medicine Platform, Andalusian Public Foundation Progress and Health-FPS, Sevilla, Spain; Institute of Biomedicine of Seville, IBiS, University Hospital Virgen del Rocío/CSIC/University of Seville, Seville, Spain; FPS/ELIXIR-ES, Andalusian Public Foundation Progress and Health-FPS, Sevilla, Spain 
Section
ORIGINAL RESEARCH
Publication year
2024
Publication date
Mar 2024
Publisher
John Wiley & Sons, Inc.
e-ISSN
23988835
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3003361370
Copyright
© 2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.