Abstract

Background

Oocyte quality is critical for the mammalian reproduction due to its necessity on fertilization and early development. During aging, the declined oocytes showing with organelle dysfunction and oxidative stress lead to infertility. AMP-activated protein kinase (AMPK) is a serine/threonine protein kinase which is important for energy homeostasis for metabolism. Little is known about the potential relationship between AMPK with oocyte aging.

Results

In present study we reported that AMPK was related with low quality of oocytes under post ovulatory aging and the potential mechanism. We showed the altered AMPK level during aging and inhibition of AMPK activity induced mouse oocyte maturation defect. Further analysis indicated that similar with its upstream regulator PKD1, AMPK could reduce ROS level to avoid oxidative stress in oocytes, and this might be due to its regulation on mitochondria function, since loss of AMPK activity induced abnormal distribution, reduced ATP production and mtDNA copy number of mitochondria. Besides, we also found that the ER and Golgi apparatus distribution was aberrant after AMPK inhibition, and enhanced lysosome function was also observed.

Conclusions

Taken together, these data indicated that AMPK is important for the organelle function to reduce oxidative stress during oocyte meiotic maturation.

Details

Title
Loss of AMPK activity induces organelle dysfunction and oxidative stress during oocyte aging
Author
Lin-Lin, Hu; Mei-Hua Liao; Ya-Xi, Liu; Chun-Hua, Xing; Lan-Lan Nong; Feng-Lian, Yang; Shao-Chen, Sun
Pages
1-10
Section
Research
Publication year
2024
Publication date
2024
Publisher
BioMed Central
e-ISSN
1745-6150
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3054192536
Copyright
© 2024. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.