It appears you don't have support to open PDFs in this web browser. To view this file, Open with your PDF reader
Abstract
Background
The clinical efficacy of Jinchuang Ointment, a traditional Chinese medicine (TCM), in treating chronic non-healing diabetic wounds has been demonstrated over the past decades. Both in vitro and in vivo angiogenic activities have been reported for its herbal ingredients, including dragon blood from the palm tree Daemonorops draco and catechu from Uncaria gambir Roxb. Additionally, crude extracts of dragon blood have exhibited hypoglycemic effects not only in animal studies but also in cell-based in vitro assays.
Results
Our findings indicate that crude dragon blood extract promotes the differentiation of myoblasts into myotubes. Partially purified fractions of dragon blood crude extract significantly enhance the expression of muscle cell differentiation-related genes such as myoG, myoD, and myoHC. Our results also demonstrate that crude extracts of dragon blood can inhibit platelet-derived growth factor-induced PAI-1 expression in primary rat vascular smooth muscle cells, thereby favoring changes in hemostasis towards fibrinolysis. Consistent with previous reports, reduced expression of plasminogen activator inhibitor 1 (PAI-1) accelerates wound healing. However, further separation resulted in a significant loss of both activities, indicating the involvement of more than one compound in these processes. Stem cells play a crucial role in muscle injury repair. Neither dragon blood nor catechu alone stimulated the proliferation of human telomerase reverse transcriptase (hTERT)-immortalized and umbilical cord mesenchymal stem cells. Interestingly, the proliferation of both types of stem cells was observed when crude extracts of dragon blood and catechu were present together in the stem cell growth medium.
Conclusions
Dragon blood from D. draco offers multifaceted therapeutic benefits for treating chronic nonhealing diabetic wounds from various perspectives. Most drugs in Western medicine consist of small molecules with defined ingredients. However, this is not the case in TCM, as the activities of dragon blood reported in this study. Surprisingly, the activities documented here align with descriptions in ancient Chinese medical texts dating back to A.D. 1625.
You have requested "on-the-fly" machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Show full disclaimer
Neither ProQuest nor its licensors make any representations or warranties with respect to the translations. The translations are automatically generated "AS IS" and "AS AVAILABLE" and are not retained in our systems. PROQUEST AND ITS LICENSORS SPECIFICALLY DISCLAIM ANY AND ALL EXPRESS OR IMPLIED WARRANTIES, INCLUDING WITHOUT LIMITATION, ANY WARRANTIES FOR AVAILABILITY, ACCURACY, TIMELINESS, COMPLETENESS, NON-INFRINGMENT, MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. Your use of the translations is subject to all use restrictions contained in your Electronic Products License Agreement and by using the translation functionality you agree to forgo any and all claims against ProQuest or its licensors for your use of the translation functionality and any output derived there from. Hide full disclaimer
Details


1 Tzu Chi University, Department of Biomedical Sciences and Engineering, Hualien, Taiwan (GRID:grid.411824.a) (ISNI:0000 0004 0622 7222)
2 Tzu Chi University, Department of Laboratory Medicine and Biotechnology, Hualien, Taiwan (GRID:grid.411824.a) (ISNI:0000 0004 0622 7222)
3 Tzu Chi University, Department of Biochemistry, Hualien City, Taiwan (GRID:grid.411824.a) (ISNI:0000 0004 0622 7222)
4 Hualien Tzu Chi Hospital, Integration Center of Traditional Chinese and Modern Medicine, Hualien, Taiwan (GRID:grid.411824.a)
5 Hualien Tzu Chi Hospital, Integration Center of Traditional Chinese and Modern Medicine, Hualien, Taiwan (GRID:grid.411824.a); Tzu Chi University, School of Post-Baccalaureate Chinese Medicine, Hualien, Taiwan (GRID:grid.411824.a) (ISNI:0000 0004 0622 7222); Hualien Tzu Chi Hospital, Department of Chinese Medicine, Hualien, Taiwan (GRID:grid.411824.a)
6 Tzu Chi University, Department of Biochemistry, Hualien City, Taiwan (GRID:grid.411824.a) (ISNI:0000 0004 0622 7222); Hualien Tzu Chi Hospital, Integration Center of Traditional Chinese and Modern Medicine, Hualien, Taiwan (GRID:grid.411824.a)