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© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Transforming growth factor beta (TGF-β) is ubiquitously found in bone and plays a key role in bone turnover. Mice expressing constitutively active TGF-β receptor type I (Mx1;TβRICA mice) are osteopenic. Here, we identified the candidate genes involved in bone turnover in Mx1;TβRICA mice using RNA sequencing analysis. A total of 285 genes, including 87 upregulated and 198 downregulated genes, were differentially expressed. According to the KEGG analysis, some genes were involved in osteoclast differentiation (Fcgr4, Lilrb4a), B cell receptor signaling (Cd72, Lilrb4a), and neutrophil extracellular trap formation (Hdac7, Padi4). Lilrb4 is related to osteoclast inhibition protein, whereas Hdac7 is a Runx2 corepressor that regulates osteoblast differentiation. Silencing Lilrb4 increased the number of osteoclasts and osteoclast marker genes. The knocking down of Hdac7 increased alkaline phosphatase activity, mineralization, and osteoblast marker genes. Therefore, our present study may provide an innovative idea for potential therapeutic targets and pathways in TβRI-associated bone loss.

Details

Title
Differential Gene Expression Involved in Bone Turnover of Mice Expressing Constitutively Active TGFβ Receptor Type I
Author
Myint, Ohnmar 1   VIAFID ORCID Logo  ; Sakunrangsit, Nithidol 1   VIAFID ORCID Logo  ; Pholtaisong, Jatuphol 1   VIAFID ORCID Logo  ; Toejing, Parichart 1 ; Pho-on, Pinyada 1 ; Asada Leelahavanichkul 2   VIAFID ORCID Logo  ; Sridurongrit, Somyoth 3 ; Aporntewan, Chatchawit 4   VIAFID ORCID Logo  ; Greenblatt, Matthew B 5 ; Lotinun, Sutada 1   VIAFID ORCID Logo 

 Center of Excellence in Skeletal Disorders and Enzyme Reaction Mechanism, Department of Physiology, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand; [email protected] (O.M.); [email protected] (N.S.); [email protected] (J.P.); [email protected] (P.T.); [email protected] (P.P.-o.) 
 Division of Immunology, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand; [email protected] 
 Department of Anatomy, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; [email protected] 
 Department of Mathematics and Computer Science, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; [email protected] 
 Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA; [email protected]; Research Division, Hospital for Special Surgery, New York, NY 10065, USA 
First page
5829
Publication year
2024
Publication date
2024
Publisher
MDPI AG
ISSN
16616596
e-ISSN
14220067
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3067476777
Copyright
© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.