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© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

This article presents the distinctive butyrylcholinesterase (BChE) fluorescent probe P5, designed via a targeting-site method. This method was proposed to enhance the affinity of the probe for BChE by targeting the peripheral anionic site (PAS) of BChE. By mimicking the natural substrate butyrylcholine, the structure of the probe was optimized by introducing a positive charge. Fluorescent probe P5, selected from a series of designed fluorescent probes P1P6, exhibited excellent affinity and specificity towards BChE, enabling rapid detection within 5 min with a low detection limit of 16.7 ng/mL. Furthermore, this probe can distinguish between normal cells and Alzheimer’s disease (AD) model cells, and demonstrated good imaging results in a P12 cell AD model. The results of this study indicate that this novel fluorescent probe could serve as a promising tool for the rapid detection of BChE and accurate AD diagnosis.

Details

Title
Improved Affinity: A Customized Fluorescent Probe for the Rapid Detection of Butyrylcholinesterase
Author
Wang, Wei 1 ; Xiao-Fei, Chen 1 ; Zhang, Yi 2 ; Yang, Ran 1 ; Long, Jin 1 ; Li, Shuai 3 ; Bai-Ou Guan 1 

 Guangdong Provincial Key Laboratory of Optical Fiber Sensing and Communication, Institute of Photonics Technology, Jinan University, Guangzhou 510632, China; [email protected] (W.W.); [email protected] (X.-F.C.); [email protected] (Y.R.); [email protected] (L.J.); College of Physics & Optoelectronic Engineering, Jinan University, Guangzhou 510632, China 
 College of Life Science and Technology, Jinan University, Guangzhou 510632, China; [email protected] 
 Special Needs Medical Center, The Eighth Affiliated Hospital of Sun Yat-sen University, Shenzhen 518033, China 
First page
100
Publication year
2024
Publication date
2024
Publisher
MDPI AG
e-ISSN
22279040
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3072274633
Copyright
© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.