Full text

Turn on search term navigation

© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

To better understand the mechanism of action of the compounds in the ethanolic extracts of J. nigra leaves and green husks, their binding to CT-DNA was investigated. This study was conducted to elucidate the in vitro protective effect of extracts against chromosomal damage in mitogen-induced human lymphocytes and investigate the possible application of selec+ted extracts as a natural source of polyphenolic compounds. Using HPLC-MS analysis, 103 different compounds were identified as having a higher number of active species, which is consistent with their activity. The frequency of micronuclei (MN) was scored in binucleated cells, and the nuclear proliferation index was calculated. Cyclic voltammetry experiments demonstrate that the nature of the interaction between extracts and CT-DNA is a synergy of electrostatic and intercalative modes, where leaves extracts showed a higher ability to bind to DNA. Extracts showed excellent antioxidant activity. At a concentration of only 4 µg/mL, extract of J. nigra leaves and the green husks reduced the incidence of MN by 58.2% and 64.5%, respectively, compared to control cell cultures.

Details

Title
Chemical Composition and Protective Possibilities of Juglans Nigra Leaves and Green Husks Extracts: DNA Binding and Micronucleus Assay in Human Lymphocytes
Author
Rajković, Katarina M 1 ; Stanković, Miroslava 2 ; Markićević, Milan 3   VIAFID ORCID Logo  ; Zavišić, Gordana 4   VIAFID ORCID Logo  ; Vranješ-Đurić, Sanja 5 ; Janković, Drina 5   VIAFID ORCID Logo  ; Obradović, Zorica 5 ; Stanković, Dalibor 6   VIAFID ORCID Logo 

 The Academy of Applied Preschool Teaching and Health Studies, Kosančićeva 36, 37000 Kruševac, Serbia; [email protected] 
 Nuclear Facilities of Serbia, Mike Petrovića Alasa 12-14, Vinča, 11001 Belgrade, Serbia 
 Institute for Oncology and Radiology of Serbia (IORS), Department of Radiotherapy Physics, Pasterova 14, 11000 Belgrade, Serbia 
 Faculty of Pharmacy, University Business Academy Novi Sad, Trg mladenaca 5, 21101 Novi Sad, Serbia; [email protected] 
 “Vinča” Institute of Nuclear Sciences—National Institute of the Republic of Serbia, University of Belgrade, Serbia, POB 522, 11001 Belgrade, Serbia[email protected] (Z.O.) 
 “Vinča” Institute of Nuclear Sciences—National Institute of the Republic of Serbia, University of Belgrade, Serbia, POB 522, 11001 Belgrade, Serbia[email protected] (Z.O.); Faculty of Chemistry, University of Belgrade, Studentski Trg 12-16, 11000 Belgrade, Serbia 
First page
1669
Publication year
2024
Publication date
2024
Publisher
MDPI AG
e-ISSN
22237747
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3072658481
Copyright
© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.