Abstract

Aedes aegypti and Aedes albopictus are the main vectors of arboviruses such as Dengue, Chikungunya and Zika, causing a major impact on global economic and public health. The main way to prevent these diseases is vector control, which is carried out through physical and biological methods, in addition to environmental management. Although chemical insecticides are the most effective strategy, they present some problems such as vector resistance and ecotoxicity. Recent research highlights the potential of the imidazolium salt "1-methyl-3-octadecylimidazolium chloride" (C18MImCl) as an innovative and environmentally friendly solution against Ae. aegypti. Despite its promising larvicidal activity, the mode of action of C18MImCl in mosquito cells and tissues remains unknown. This study aimed to investigate its impacts on Ae. aegypti larvae and three cell lines of Ae. aegypti and Ae. albopictus, comparing the cellular effects with those on human cells. Cell viability assays and histopathological analyses of treated larvae were conducted. Results revealed the imidazolium salt’s high selectivity (> 254) for mosquito cells over human cells. After salt ingestion, the mechanism of larval death involves toxic effects on midgut cells. This research marks the first description of an imidazolium salt's action on mosquito cells and midgut tissues, showcasing its potential for the development of a selective and sustainable strategy for vector control.

Details

Title
Imidazolium salt’s toxic effects in larvae and cells of Aedes aegypti and Aedes albopictus (Diptera: Culicidae)
Author
da Silva, Wellington Junior 1 ; Diel, Leonardo Francisco 2 ; Pilz-Júnior, Harry Luiz 1 ; de Lemos, Alessandra Bittencourt 1 ; de Freitas Milagres, Tarcísio 1 ; Pereira, Igor Luiz Gonçalves 3 ; Bernardi, Lisiane 4 ; Ribeiro, Bergmann Morais 5 ; Lamers, Marcelo Lazzaron 4 ; Schrekker, Henri Stephan 3 ; da Silva, Onilda Santos 1 

 Universidade Federal do Rio Grande do Sul, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Porto Alegre, Brazil (GRID:grid.8532.c) (ISNI:0000 0001 2200 7498) 
 Universidade Federal do Rio Grande do Sul, Faculty of Dentistry, Porto Alegre, Brazil (GRID:grid.8532.c) (ISNI:0000 0001 2200 7498) 
 Universidade Federal do Rio Grande do Sul, Laboratory of Technological Processes and Catalysis, Institute of Chemistry, Porto Alegre, Brazil (GRID:grid.8532.c) (ISNI:0000 0001 2200 7498) 
 Universidade Federal do Rio Grande do Sul, Department of Morphological Sciences, Institute of Basic Health Sciences, Porto Alegre, Brazil (GRID:grid.8532.c) (ISNI:0000 0001 2200 7498) 
 Universidade de Brasília, Department of Celular Biology, Institute of Biological Sciences, Brasília-DF, Brazil (GRID:grid.7632.0) (ISNI:0000 0001 2238 5157) 
Pages
15421
Publication year
2024
Publication date
2024
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3075790067
Copyright
© The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.