Abstract

Investigating ligand-protein complexes is essential in the areas of chemical biology and drug discovery. However, detailed information on key reagents such as fluorescent tracers and associated data for the development of widely used bioluminescence resonance energy transfer (BRET) assays including NanoBRET, time-resolved Förster resonance energy transfer (TR-FRET) and fluorescence polarization (FP) assays are not easily accessible to the research community. We created tracerDB, a curated database of validated tracers. This resource provides an open access knowledge base and a unified system for tracer and assay validation. The database is freely available at https://www.tracerdb.org/.

Tracers are fluorescent protein ligands required for various displacement assays. Here, the authors announce a curated database named tracerDB, which will make essential tracer data, contributed by the worldwide research community, easily available and searchable.

Details

Title
tracerDB: a crowdsourced fluorescent tracer database for target engagement analysis
Author
Dopfer, Johannes 1   VIAFID ORCID Logo  ; Vasta, James D. 2 ; Müller, Susanne 1   VIAFID ORCID Logo  ; Knapp, Stefan 3 ; Robers, Matthew B. 2   VIAFID ORCID Logo  ; Schwalm, Martin P. 3   VIAFID ORCID Logo 

1  Goethe University Frankfurt, Institute of Pharmaceutical Chemistry, Frankfurt am Main, Germany (GRID:grid.7839.5) (ISNI:0000 0004 1936 9721); Buchmann Institute for Life Sciences, Structural Genomics Consortium, Goethe University Frankfurt, Frankfurt am Main, Germany (GRID:grid.7839.5) (ISNI:0000 0004 1936 9721) 
2  Promega Corporation, Madison, USA (GRID:grid.418773.e) (ISNI:0000 0004 0430 2735) 
3  Goethe University Frankfurt, Institute of Pharmaceutical Chemistry, Frankfurt am Main, Germany (GRID:grid.7839.5) (ISNI:0000 0004 1936 9721); Buchmann Institute for Life Sciences, Structural Genomics Consortium, Goethe University Frankfurt, Frankfurt am Main, Germany (GRID:grid.7839.5) (ISNI:0000 0004 1936 9721); DKTK Site Frankfurt-Mainz, German Cancer Consortium (DKTK)/German Cancer Research Center (DKFZ), Heidelberg, Germany (GRID:grid.7497.d) (ISNI:0000 0004 0492 0584) 
Pages
5646
Publication year
2024
Publication date
2024
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41467-024-49896-5
ProQuest document ID
3076105101
Copyright
© The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.