Antibacterial efficacy of decorated carbon nanotubes by nano silver against pseudomonas aeruginosa

Abstract

Carbon nanotubes (CNTs) are considered as a promising nanomaterial for a variety of applications. It has desirable physicochemical characteristics of high surface area, superior mechanical and thermal strength, and electrochemical activity. In this study, CNTs decorated with silver nanoparticles (AgNPs) were manufactured by an arc discharge technique. As a result, etching produces stable AgNPs-CNTs aqueous suspensions of pure silver and carbon electrodes in ethanol vapor condensed in water. UV- visible photometer, x-ray Diffraction, transmission electron microscope, and energy dispersive x-ray characterized the synthesized AgNPs-CNTs. The synthesized AgNPs-CNTs crystals showed CNTs formation of an average 9.5 nm, with intermediate length position of about 75.8 nm decorated by homogeneous spherical AgNPs of average size 15.2 nm. The antibacterial efficiency of AgNPs-CNTs against Pseudomonas aeruginosa was established at a series of concentrations (45 g ml⁻¹: 0.0879 g ml⁻¹) while values for the MIC and MBC were determined. The MIC and MBC levels were found to be (>5.625 μg ml⁻¹) and (>11.25 μg/ml) respectively. The bacteria cytotoxicity was evaluated through LDH and protein leakage levels. Treated samples with 3.2 μg ml⁻¹ AgNPs-CNTs revealed significant injuries in the cell membrane by two times greater in LDH and protein leakage levels than samples treated by 5.0 μg ml⁻¹ AgNPs. Results in this work substantiate the synergistic effect of combining AgNPs with CNTs to enhanced antibacterial properties and performance compared to AgNPs alone. The efficiency of using synthesized AgNPs-CNTs showed high antibacterial potential against Pseudomonas aeruginosa in a concentration-dependent manner. The arc discharge method can be adapted to incorporate different materials or change the synthesis conditions, allowing for the production of AgNPs-CNTs with tailored properties for specific applications.