Full text

Turn on search term navigation

© 2023. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Background

Astigmatic mites contain potent allergens that can trigger IgE-mediated immune responses, leading to allergic diseases such as asthma, allergic rhinitis and atopic dermatitis. In house dust mites Dermatophagoides pteronyssinus and Dermatophagoides farinae, group 1 allergens (Der p 1 and Der f 1), characterized as papain-like cysteine proteases, have been defined as the major allergens that have high prevalence and potency. Previous studies of mite group 1 allergens mainly focused on identification, comparison of sequence and structure, as well as the investigation of cross-reactivity. To achieve a comprehensive view of mite group 1 allergens, we performed a comparative genomic analysis of all the cysteine proteases in six astigmatic mite species to elucidate the evolutionary relationships of group 1 allergens.

Methods

Based on the high-quality and annotated genomes, all the cysteine proteases in six astigmatic mite species were identified by sequence homology search. The phylogenetic relationships, gene synteny and expression levels were revealed by bioinformatic tools. The allergenicity of recombinant cysteine proteases was evaluated by enzyme-linked immunosorbent assay.

Results

Tandem duplication was revealed as the major feature of cysteine protease gene evolution in astigmatic mites. The high IgE-binding capacity and the significant expression level of the cysteine protease DP_007902.01 suggested its potential as a novel group 1 allergen of D. pteronyssinus. In addition, gene decay events were identified in the skin-burrowing parasitic mite Sarcoptes scabiei.

Conclusion

This comprehensive analysis provided insights into the evolution of cysteine proteases, as well as the component-resolved diagnosis of mite allergies.

Details

Title
Comparative analysis of cysteine proteases reveals gene family evolution of the group 1 allergens in astigmatic mites
Author
Shi, Ling 1   VIAFID ORCID Logo  ; Xiong, Qing 2 ; Fu Kiu Ao 1 ; Tsz Yau Wan 1 ; Xiao, Xiaojun 3 ; Liu, Xiaoyu 3 ; Sun, Baoqing 4 ; Tungtrongchitr, Anchalee 5 ; Ting Fan Leung 6 ; Stephen Kwok Wing Tsui 7 

 School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, China; Hong Kong Bioinformatics Centre, The Chinese University of Hong Kong, Hong Kong, China 
 School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, China; Hong Kong Bioinformatics Centre, The Chinese University of Hong Kong, Hong Kong, China; Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hong Kong, China 
 Shenzhen Key Laboratory of Allergy and Immunology, School of Medicine, Shenzhen University, Shenzhen, China 
 State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China 
 Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand 
 Department of Paediatrics, The Chinese University of Hong Kong, Hong Kong, China 
 School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, China; Hong Kong Bioinformatics Centre, The Chinese University of Hong Kong, Hong Kong, China; Centre for Microbial Genomics and Proteomics, The Chinese University of Hong Kong, Hong Kong, China 
Section
ORIGINAL ARTICLE
Publication year
2023
Publication date
Dec 2023
Publisher
John Wiley & Sons, Inc.
e-ISSN
20457022
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3084304122
Copyright
© 2023. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.