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Abstract
The cellular response to cisplatin was assessed in human osteosarcoma cells, using synchrotron-based (SR) Fourier Transform InfraRed nanospectroscopy (nano-FTIR) at the MIRIAM beamline B22 of Diamond Light Source (UK). This label-free mapping method delivered simultaneous morphological and biochemical information on a subcellular level (i.e. 100 s nanometer or better). Based on specific spectral biomarkers, the main biochemical constituents affected by the drug were identified at distinct locations within the cell´s inner body. Cisplatin was shown to have a noteworthy effect on proteins, mostly within the cytoplasm. A clear drug impact on cellular lipids was also observed. Within current literature on s-SNOM, this nanospectroscopy work represents a first successful application in life sciences providing full fingerprint nano-FTIR spectra across intact human cancer cells.
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Details
1 University of Coimbra, Department of Chemistry, Química-Física Molecular, Coimbra, Portugal (GRID:grid.8051.c) (ISNI:0000 0000 9511 4342)
2 Harwell Science and Innovation Campus, Diamond Light Source, Chilton - Didcot, UK (GRID:grid.18785.33) (ISNI:0000 0004 1764 0696); University of Oxford, Department of Engineering Science, Oxford, UK (GRID:grid.4991.5) (ISNI:0000 0004 1936 8948)
3 Harwell Science and Innovation Campus, Diamond Light Source, Chilton - Didcot, UK (GRID:grid.18785.33) (ISNI:0000 0004 1764 0696)
4 University of Coimbra, Department of Chemistry, Química-Física Molecular, Coimbra, Portugal (GRID:grid.8051.c) (ISNI:0000 0000 9511 4342); University of Coimbra, Department of Life Sciences, Coimbra, Portugal (GRID:grid.8051.c) (ISNI:0000 0000 9511 4342)