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Abstract
In order to investigate the changes in the properties of the cell culture solution in the effect of cell synchronization via cell starvation (for 12, 24, and 36 h), a new spiral-interdigital pattern of microelectrode as a biosensor has been proposed. Then, to test its superiority, the results of this spiral-interdigital pattern with the results of the commercial pattern have been compared. The cells were selected from breast cancer standard lines (MDA-MB-231). Changes in CV peaks of the secretions were recorded by the spiral-interdigital pattern, in which increasing the interactive surface with homogenous electric paths had been considered by simulation before fabrication. The results of the simulation and experimental procedures showed a meaningful correlation. The occurrence of CV oxidative peaks at about 0.1–0.4 V and reductive peaks at approximately 0 V in the spiral-interdigital biosensor in the starved MDA-MB-231 cell line has been observed. The starvation situation resembles one that does not cause meaningful cell apoptosis or necrosis, and this method is only used to make the cells synchronized. Also, no peak is observed in normal cell growth conditions. In addition, by using the commercial design of the electrodes, no peak is observed in any of the conditions of normal and synchronized growth of the cells. Therefore, it seems that the observed peaks are caused by the agents that are secreted in the cell culture solution in a synchronized situation. Moreover, the design of the new spiral-interdigital electrode can significantly increase the sensitivity of the sensor to receive these peaks due to more space and a uniform electric field.
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1 College of Engineering, University of Tehran, Nano Electronic Center of Excellence, Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, Tehran, Iran (GRID:grid.46072.37) (ISNI:0000 0004 0612 7950); Payame Noor University (PNU), Department of Physics, Tehran, Iran (GRID:grid.412462.7) (ISNI:0000 0000 8810 3346); University of Tehran, Nano Bio Electronic Devices Lab, Cancer Electronic Research Group, School of Electrical and Computer Engineering, College of Engineering, Tehran, Iran (GRID:grid.46072.37) (ISNI:0000 0004 0612 7950)
2 College of Engineering, University of Tehran, Nano Electronic Center of Excellence, Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, Tehran, Iran (GRID:grid.46072.37) (ISNI:0000 0004 0612 7950); University of Tehran, Nano Electronic Center of Excellence, Thin Film and Nano Electronics Lab, School of Electrical and Computer Engineering, Tehran, Iran (GRID:grid.46072.37) (ISNI:0000 0004 0612 7950); Tehran University of Medical Sciences, UT and TUMS Cancer Electronics Research Center, Tehran, Iran (GRID:grid.411705.6) (ISNI:0000 0001 0166 0922); University of Tehran, Nano Bio Electronic Devices Lab, Cancer Electronic Research Group, School of Electrical and Computer Engineering, College of Engineering, Tehran, Iran (GRID:grid.46072.37) (ISNI:0000 0004 0612 7950); Tehran University of Medical Sciences, Institute of Cancer, Imam Khomeini Hospital, Tehran, Iran (GRID:grid.411705.6) (ISNI:0000 0001 0166 0922)
3 Shahid Beheshti University, Department of Physics, Tehran, Iran (GRID:grid.412502.0) (ISNI:0000 0001 0686 4748)
4 College of Engineering, University of Tehran, Nano Electronic Center of Excellence, Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, Tehran, Iran (GRID:grid.46072.37) (ISNI:0000 0004 0612 7950); University of Tehran, Nano Bio Electronic Devices Lab, Cancer Electronic Research Group, School of Electrical and Computer Engineering, College of Engineering, Tehran, Iran (GRID:grid.46072.37) (ISNI:0000 0004 0612 7950)