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© 2023. This work is published under http://creativecommons.org/licenses/by/4.0/ (the "License"). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Background

The direct application of umbilical cord‐derived mesenchymal stem cells (UCMSCs) for promoting skin wound healing and regeneration is challenging due to strict maintenance requirements and unpredictable differentiation results.

Methods

We developed dead but functional liquid nitrogen‐treated UCMSCs (LNT‐MSCs) by rapidly immersing live UCMSCs (live‐MSCs) in liquid nitrogen.

Results

The LNT‐MSCs maintained similar cellular structures and surface markers to those of live‐MSCs. We evaluated the therapeutic effects of both live‐MSCs and LNT‐MSCs on full‐thickness skin wound healing in rats. Our results showed that the LNT‐MSCs accelerated wound closure by enhancing the proliferation and migration of skin cells, promoting angiogenesis, and inducing a favourable macrophage phenotype shift. The regenerative healing effect of LNT‐MSCs was comparable to that of live‐MSCs, making them a potential alternative strategy for accelerating wound closure that avoids unpredictable differentiation results and creates a ready‐to‐use cell bank for clinical applications.

Details

Title
Dead but functional liquid nitrogen‐treated umbilical cord mesenchymal stem cells accelerate full‐thickness skin wound healing
Author
Yang, Yan 1 ; Feng, Yuqing 2 ; Hu, Meirong 3 ; Deng, Jingxian 2 ; Wei, Siying 3 ; Wang, Zhaoyang 3 ; Li, Lu 3 ; Huang, Rufei 3 ; Wang, Yuxin 3 ; Liu, Yuan 3 ; Ye, Tao 3 ; Huang, Yadong 4   VIAFID ORCID Logo 

 Guangdong Province Key Laboratory of Bioengineering Medicine, Guangzhou, China 
 Department of Pharmacology, Jinan University, Guangzhou, China 
 Department of Cell Biology, Jinan University, Guangzhou, China 
 Guangdong Engineering Research Centre for Medical Beauty Materials and Effective Skin Care Products, Guangzhou, China 
Section
RESEARCH ARTICLES
Publication year
2023
Publication date
Aug 1, 2023
Publisher
John Wiley & Sons, Inc.
ISSN
27680622
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3090347274
Copyright
© 2023. This work is published under http://creativecommons.org/licenses/by/4.0/ (the "License"). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.