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© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Food safety control is a key issue in the food and agriculture industries. For such purposes, developing miniaturized analytical methods is critical for enabling the rapid and sensitive detection of food supplements, allergens, and pollutants. Here, a novel bioanalytical methodology based on DNA-functionalized gold nanoparticles (AuNPs) and colorimetric detection was developed to detect the presence of sesame (a major allergen) through sesame seed DNA as a target, in food samples. The presence of sesame DNA induces controlled nanoparticle aggregation/desegregation, resulting in a color change (from blue to red) proportional to sesame DNA concentration. The incorporation of multicomponent nucleic acid enzymes (MNAzymes) in this strategy has been carried out to perform an isothermal signal amplification strategy to improve the sensitivity of detection. Also, open-source software for color analysis was used to ensure an unbiased visual color-change detection, enhancing detection accuracy and sensitivity and opening the possibility of performing a simple and decentralized analyte detection. The method successfully detected the presence of sesame DNA in sesame seed, sesame oil, olive oil, and sunflower oil. In brief, the developed approach constitutes a simple and affordable alternative to perform a highly sensitive detection of DNA in food without complex methodologies or the requirement of expensive instrumentation.

Details

Title
Sesame Detection in Food Using DNA-Functionalized Gold Nanoparticles: A Sensitive, Rapid, and Cost-Effective Colorimetric Approach
Author
Llano-Suárez, Pablo 1   VIAFID ORCID Logo  ; Sánchez-Visedo, Adrián 1   VIAFID ORCID Logo  ; Ortiz-Gómez, Inmaculada 1   VIAFID ORCID Logo  ; Fernández-Argüelles, María Teresa 1   VIAFID ORCID Logo  ; Prado, Marta 2   VIAFID ORCID Logo  ; Costa-Fernández, José Manuel 1   VIAFID ORCID Logo  ; Soldado, Ana 1   VIAFID ORCID Logo 

 Department of Physical and Analytical Chemistry, University of Oviedo, c/Julián Clavería, 8, 33006 Oviedo, Spain[email protected] (A.S.-V.); [email protected] (M.T.F.-A.); [email protected] (J.M.C.-F.) 
 International Iberian Nanotechnology Laboratory, Av. Mestre José Veiga Sthis n, 4715-330 Braga, Portugal; [email protected] 
First page
377
Publication year
2024
Publication date
2024
Publisher
MDPI AG
e-ISSN
20796374
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3097851712
Copyright
© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.