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© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Human cytomegalovirus (HCMV) infection remains a major complication for solid organ transplant recipients (SOTRs). The aim of this study was to evaluate the role of HCMV-specific T cell immunity measured at the time of the HCMV-DNA peak in predicting the spontaneous clearance of infection. The performance of cytokine flow cytometry using infected dendritic cells (CFC-iDC), infected cell lysate (CFC-iCL) and pp65 peptide pool (CFC-pp65 pool) as stimuli, as well as ELISPOT assays using infected cell lysate (ELISPOT-iCL) and the pp65 peptide pool (ELISPOT-pp65 pool), was analysed. Among the 40 SOTRs enrolled, 16 patients (40%) required antiviral treatment for an HCMV infection (Non-Controllers), while the others spontaneously cleared the infection (Controllers). At the HCMV-DNA peak, the number of HCMV-specific CD4+ T cells detected by the CFC-iDC, CFC-iCL and CFC-pp65 pool assays in Controllers was higher than that detected in Non-Controllers, while no difference was observed in terms of HCMV-specific CD8+ T cell response. The same trend was observed when the HCMV-specific T cell response was measured by ELISPOT-iCL and ELISPOT-pp65 pool. We observed that the CD4+ CFC-pp65 pool assay was the best predictor of self-resolving HCMV infection at the time of the HCVM-DNA peak. The CFC-pp65 pool assay is able to discriminate between CD4+ and CD8+ T cell responses and could be used in daily clinical practice.

Details

Title
Immune Control of Human Cytomegalovirus (HCMV) Infection in HCMV-Seropositive Solid Organ Transplant Recipients: The Predictive Role of Different Immunological Assays
Author
Zavaglio, Federica 1 ; Cassaniti, Irene 2   VIAFID ORCID Logo  ; Piera d’Angelo 1   VIAFID ORCID Logo  ; Zelini, Paola 1   VIAFID ORCID Logo  ; Comolli, Giuditta 1 ; Gregorini, Marilena 3   VIAFID ORCID Logo  ; Rampino, Teresa 4   VIAFID ORCID Logo  ; Lucia Del Frate 5 ; Meloni, Federica 5 ; Pellegrini, Carlo 6   VIAFID ORCID Logo  ; Abelli, Massimo 7   VIAFID ORCID Logo  ; Ticozzelli, Elena 7 ; Lilleri, Daniele 1   VIAFID ORCID Logo  ; Baldanti, Fausto 2 

 Microbiology and Virology Unit, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy; [email protected] (F.Z.); [email protected] (P.d.); [email protected] (P.Z.); [email protected] (G.C.); [email protected] (D.L.); [email protected] (F.B.) 
 Microbiology and Virology Unit, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy; [email protected] (F.Z.); [email protected] (P.d.); [email protected] (P.Z.); [email protected] (G.C.); [email protected] (D.L.); [email protected] (F.B.); Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of Pavia, 27100 Pavia, Italy; [email protected] 
 Unit of Nephrology, Dialysis and Transplantation, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy; [email protected] (M.G.); [email protected] (T.R.); Department of Internal Medicine and Therapeutics, University of Pavia, 27100 Pavia, Italy 
 Unit of Nephrology, Dialysis and Transplantation, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy; [email protected] (M.G.); [email protected] (T.R.) 
 Transplant Centre Unit, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy; [email protected] (L.D.F.); [email protected] (F.M.) 
 Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of Pavia, 27100 Pavia, Italy; [email protected]; Cardiac Surgery, Department of Intensive Medicine, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy 
 Department of Surgery, University of Pavia, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy; [email protected] (M.A.); [email protected] (E.T.) 
First page
1325
Publication year
2024
Publication date
2024
Publisher
MDPI AG
e-ISSN
20734409
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3097857311
Copyright
© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.