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© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Conjugated linoleic acid (CLA) is a class of bioactive fatty acids that exhibit various physiological activities such as anti-cancer, anti-atherosclerosis, and lipid-lowering. It is an essential fatty acid that cannot be synthesized by the human body and must be derived from dietary sources. The natural sources of CLA are limited, predominantly relying on chemical and enzymatic syntheses methods. Microbial biosynthesis represents an environmentally benign approach for CLA production. Pine nut oil, containing 40–60% linoleic acid, serves as a promising substrate for CLA enrichment. In the present study, we developed a novel method for the production of CLA from pine nut oil using Lactiplantibacillus plantarum (L. plantarum) Lp-01, which harbors a linoleic acid isomerase. The optimal fermentation parameters for CLA production were determined using a combination of single-factor and response surface methodologies: an inoculum size of 2%, a fermentation temperature of 36 °C, a fermentation time of 20 h, and a pine nut oil concentration of 11%. Under these optimized conditions, the resultant CLA yield was 33.47 μg/mL. Gas chromatography analysis revealed that the fermentation process yielded a mixture of c9, t11CLA and t10, c12 CLA isomers, representing 4.91% and 4.86% of the total fatty acid content, respectively.

Details

Title
Conjugated Linoleic Acid Production in Pine Nut Oil: A Lactiplantibacillus plantarum Lp-01 Fermentation Approach
Author
Wei, Gang 1   VIAFID ORCID Logo  ; Wu, Ge 2 ; Sun, Jiajia 3 ; Qi, Yi 1 ; Zhao, Qi 1 ; Xu, Fengde 1   VIAFID ORCID Logo  ; Zhang, Zhi 4 ; Peng, Lanzhi 1 

 School of Ocean and Tropical Medicine, Guangdong Medical University, Zhanjiang 524023, China; [email protected] (G.W.); [email protected] (G.W.); [email protected] (Y.Q.); [email protected] (Q.Z.); [email protected] (F.X.); The Marine Biomedical Research Institute of Guangdong Zhanjiang, Zhanjiang 524023, China 
 School of Ocean and Tropical Medicine, Guangdong Medical University, Zhanjiang 524023, China; [email protected] (G.W.); [email protected] (G.W.); [email protected] (Y.Q.); [email protected] (Q.Z.); [email protected] (F.X.) 
 College of Forestry, Northeast Forestry University, 26 Hexing Road, Xiangfang District, Harbin 150040, China; [email protected] 
 College of Life Sciences, Northeast Forestry University, 26 Hexing Road, Xiangfang District, Harbin 150040, China 
First page
2472
Publication year
2024
Publication date
2024
Publisher
MDPI AG
e-ISSN
23048158
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3097927819
Copyright
© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.