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© 2024, Kahn et al. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Antibodies are used in many areas of biomedical and clinical research, but many of these antibodies have not been adequately characterized, which casts doubt on the results reported in many scientific papers. This problem is compounded by a lack of suitable control experiments in many studies. In this article we review the history of the ‘antibody characterization crisis’, and we document efforts and initiatives to address the problem, notably for antibodies that target human proteins. We also present recommendations for a range of stakeholders – researchers, universities, journals, antibody vendors and repositories, scientific societies and funders – to increase the reproducibility of studies that rely on antibodies.

Details

Title
Antibody characterization is critical to enhance reproducibility in biomedical research
Author
Kahn, Richard A; Virk Harvinder; Laflamme, Carl; Houston, Douglas W; Polinski, Nicole K; Meijers, Rob; Levey, Allan I; Saper, Clifford B; Errington, Timothy M; Turn, Rachel E; Bandrowski Anita; Trimmer, James S; Rego Meghan; Freedman, Leonard P; Ferrara Fortunato; Bradbury Andrew RM; Cable, Hannah; Longworth Skye
University/institution
U.S. National Institutes of Health/National Library of Medicine
Publication year
2024
Publication date
2024
Publisher
eLife Sciences Publications Ltd.
e-ISSN
2050084X
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3099261480
Copyright
© 2024, Kahn et al. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.