Abstract

Cerebrospinal fluid (CSF) plays an important role in brain tumors, including medulloblastoma (MBL). Recent advancements in mass spectrometry systems and ‘Omics’ data analysis methods enable unbiased, high proteome depth research. We conducted proteomic profiling of the total CSF in MBL patients with the purpose of finding a potential diagnostic biomarker for MBL. We quantified 1112 proteins per CSF sample. Feature selection identified four elevated soluble proteins (SPTBN1, HSP90AA1, TKT, and NME1-NME2) in MBL CSF. Validation with ELISA confirmed that TKT was significantly elevated in MBL. Additionally, TKT-positive extracellular vesicles were significantly enriched in MBL CSF and correlated with the burden of leptomeningeal seeding. Our results provide insights into the proteomics data of the total CSF of MBL patients. Furthermore, we identified the significance of TKT within the total CSF and its presence within circulating EVs in the CSF. We suggest that TKT may serve as a biomarker for MBL.

Details

Title
Proteomic profiling of cerebrospinal fluid reveals TKT as a potential biomarker for medulloblastoma
Author
Kim, Joo Whan 1 ; Choi, Seung Ah 1 ; Dan, Kisoon 2 ; Koh, Eun Jung 1 ; Ha, Saehim 1 ; Phi, Ji Hoon 3 ; Kim, Kyung Hyun 1 ; Han, Dohyun 4 ; Kim, Seung-Ki 3 

 Seoul National University Children’s Hospital, Division of Pediatric Neurosurgery, Pediatric Clinical Neuroscience Center, Seoul, South Korea (GRID:grid.412482.9) (ISNI:0000 0004 0484 7305); Seoul National University College of Medicine, Department of Neurosurgery, Seoul National University Hospital, Seoul, South Korea (GRID:grid.31501.36) (ISNI:0000 0004 0470 5905) 
 Seoul National University Hospital, Proteomics Core Facility, Biomedical Research Institute, Seoul, South Korea (GRID:grid.412484.f) (ISNI:0000 0001 0302 820X) 
 Seoul National University Children’s Hospital, Division of Pediatric Neurosurgery, Pediatric Clinical Neuroscience Center, Seoul, South Korea (GRID:grid.412482.9) (ISNI:0000 0004 0484 7305); Seoul National University College of Medicine, Department of Neurosurgery, Seoul National University Hospital, Seoul, South Korea (GRID:grid.31501.36) (ISNI:0000 0004 0470 5905); Seoul National University College of Medicine, Neuroscience Research Institute, Seoul, South Korea (GRID:grid.31501.36) (ISNI:0000 0004 0470 5905) 
 Seoul National University Hospital, Proteomics Core Facility, Biomedical Research Institute, Seoul, South Korea (GRID:grid.412484.f) (ISNI:0000 0001 0302 820X); Seoul National University Hospital, Department of Transdisciplinary Medicine, Seoul, South Korea (GRID:grid.412484.f) (ISNI:0000 0001 0302 820X) 
Pages
21053
Publication year
2024
Publication date
2024
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3102226437
Copyright
© The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.