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© 2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the "License"). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Cell‐based microarrays are widely used in the fields of drug discovery and toxicology. Precise gradient generation and automated drug feeding are essential for high‐throughput screening of live cells in tiny droplets. However, most existing technologies either require sophisticated robotic equipment or cause mechanical/physiological interference with cells. Here, a heterogeneous organohydrogel is presented for automated gradient drug feeding, while ensuring minimal interference with cells. The heterogeneous organohydrogel comprises three crucial components. The bottom surface can automatically generate gradients functioning as a gradient generator, the organohydrogel bulk allows unidirectional transport of drugs without backflow, and the top surface with hydrophilic arrays can firmly anchor the cell‐based droplet array to evaluate the concentration‐dependent bioeffects of drugs accurately. Such a unique structure enables universal screening of different cell types and drugs dissolved in different solvents, requiring neither additional accessories nor arduous drug functionalization. The heterogeneous organohydrogel with unprecedented automation and non‐interference possesses the enormous potential to be a next‐generation platform for drug screening.

Details

Title
Heterogeneous Organohydrogel Toward Automated and Interference‐Free Gradient Feeding of Drugs in Cell Screening
Author
Gao, Hongxiao 1 ; Wan, Xizi 2 ; Xiao, Wu‐Yi 2 ; Yang, Yuemeng 1 ; Lu, Jingwei 1 ; Wu, Shihao 1 ; Xu, Li‐Ping 1 ; Wang, Shutao 3   VIAFID ORCID Logo 

 Beijing Key Laboratory for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, P. R. China 
 CAS Key Laboratory of Bio‐inspired Materials and Interfacial Science, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, P. R. China, University of Chinese Academy of Sciences, Beijing, P. R. China 
 CAS Key Laboratory of Bio‐inspired Materials and Interfacial Science, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, P. R. China, University of Chinese Academy of Sciences, Beijing, P. R. China, Suzhou Institute for Advanced Research, University of Science and Technology of China, Suzhou, P. R. China 
Section
Research Article
Publication year
2024
Publication date
Oct 1, 2024
Publisher
John Wiley & Sons, Inc.
e-ISSN
21983844
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3121275907
Copyright
© 2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the "License"). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.