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© 2024. This work is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the "License"). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Objectives

This study investigates curcumin's neuroprotective role and its potential in promoting neurogenesis in progenitor cells within the brain. Notably, curcumin's antioxidant properties have been implicated in Alzheimer's disease treatment. However, the association between curcumin's antioxidative effects and its impact on neural stem cells (NSCs) remains to be elucidated.

Methods

C17.2 neural stem cells were utilized as a model to simulate oxidative stress, induced by hydrogen peroxide (H2O2). We quantified the levels of superoxide dismutase (SOD), malondialdehyde (MDA), and intracellular reactive oxygen species (ROS), alongside the gene expression of SOD1 and SOD2, to assess intracellular oxidative stress. Additionally, Western blot analysis was conducted to measure the expressions of LC3‐II, Beclin‐1, and phosphorylated ERK (p‐ERK), thereby evaluating autophagy and ERK signaling pathway activation.

Results

Treatment with curcumin resulted in a reduction of MDA and ROS levels, suggesting a protective effect on NSCs against oxidative damage induced by H2O2. Furthermore, a decrease in the relative expressions of LC3‐II, Beclin‐1, and p‐ERK was observed post‐curcumin treatment.

Conclusions

The findings suggest that curcumin may confer protection against oxidative stress by attenuating autophagy and deactivating the ERK1/2 signaling pathways, which could contribute to therapeutic strategies for Alzheimer's disease.

Details

Title
Curcumin inhibits oxidative stress and autophagy in C17.2 neural stem cell through ERK1/2 signaling pathways
Author
Ruan, Yuting 1 ; Luo, Haoyu 2 ; Tang, Jingyi 3 ; Ji, Mengyao 2 ; Yu, Dapeng 1 ; Yu, Qun 2 ; Cao, Zhiyu 3 ; Mai, Yingren 2 ; Zhang, Bei 4 ; Chen, Yan 1 ; Liu, Jun 2   VIAFID ORCID Logo  ; Liao, Wang 2   VIAFID ORCID Logo 

 Department of Rehabilitation, The Second Affiliated Hospital, Guangzhou Medical University, Guangzhou, Guangdong, China 
 Department of Neurology, The Second Affiliated Hospital, Guangzhou Medical University, Guangzhou, Guangdong, China 
 Department of Neurology, Sun Yat‐sen Memorial Hospital of Sun Yat‐sen University, Guangdong, China 
 Department of Neurology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, China 
Pages
559-570
Section
ORIGINAL ARTICLE
Publication year
2024
Publication date
Oct 1, 2024
Publisher
John Wiley & Sons, Inc.
e-ISSN
24750360
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3123932731
Copyright
© 2024. This work is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the "License"). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.