Abstract

Voltage imaging holds great potential for biomedical research by enabling noninvasive recording of the electrical activity of excitable cells such as neurons or cardiomyocytes. Camera-based detection can record from hundreds of cells in parallel, but imaging entire volumes is limited by the need to focus through the sample at high speeds. Remote focusing techniques can remedy this drawback, but have so far been either too slow or light-inefficient. Here, we introduce flipped image remote focusing, a remote focusing method that doubles the light efficiency compared to conventional beamsplitter-based techniques and enables high-speed volumetric voltage imaging at 500 volumes/s. We show the potential of our approach by combining it with light sheet imaging in the zebrafish spinal cord to record from >100 spontaneously active neurons in parallel.

The authors develop a high-speed remote focusing method for volumetric voltage imaging enabling imaging at 500 volumes/s. This is combined with light sheet microscopy to record data from >100 spontaneously active neurons in parallel.

Details

Title
Fast and light-efficient remote focusing for volumetric voltage imaging
Author
Böhm, Urs L. 1   VIAFID ORCID Logo  ; Judkewitz, Benjamin 2   VIAFID ORCID Logo 

 Charité – Universitätsmedizin Berlin, Einstein Center for Neurosciences, Berlin, Germany (GRID:grid.6363.0) (ISNI:0000 0001 2218 4662); Institute of Psychiatry and Neuroscience of Paris (IPNP), Université Paris Cité, Paris, France (GRID:grid.512035.0) 
 Charité – Universitätsmedizin Berlin, Einstein Center for Neurosciences, Berlin, Germany (GRID:grid.6363.0) (ISNI:0000 0001 2218 4662) 
Pages
9555
Publication year
2024
Publication date
2024
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3124210726
Copyright
© The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.