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Abstract
Voltage imaging holds great potential for biomedical research by enabling noninvasive recording of the electrical activity of excitable cells such as neurons or cardiomyocytes. Camera-based detection can record from hundreds of cells in parallel, but imaging entire volumes is limited by the need to focus through the sample at high speeds. Remote focusing techniques can remedy this drawback, but have so far been either too slow or light-inefficient. Here, we introduce flipped image remote focusing, a remote focusing method that doubles the light efficiency compared to conventional beamsplitter-based techniques and enables high-speed volumetric voltage imaging at 500 volumes/s. We show the potential of our approach by combining it with light sheet imaging in the zebrafish spinal cord to record from >100 spontaneously active neurons in parallel.
The authors develop a high-speed remote focusing method for volumetric voltage imaging enabling imaging at 500 volumes/s. This is combined with light sheet microscopy to record data from >100 spontaneously active neurons in parallel.
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1 Charité – Universitätsmedizin Berlin, Einstein Center for Neurosciences, Berlin, Germany (GRID:grid.6363.0) (ISNI:0000 0001 2218 4662); Institute of Psychiatry and Neuroscience of Paris (IPNP), Université Paris Cité, Paris, France (GRID:grid.512035.0)
2 Charité – Universitätsmedizin Berlin, Einstein Center for Neurosciences, Berlin, Germany (GRID:grid.6363.0) (ISNI:0000 0001 2218 4662)