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© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Lectin extracted from Amaranthus hypochondriacus was purified using an affinity column with an agarose-fetuin matrix specific to the lectin of interest. Purification was confirmed by SDS-PAGE, revealing a single protein band with a molecular mass of 34.4 kDa. A hemagglutination assay showed that the lectin had a higher affinity for human type A erythrocytes, and its hemagglutinating activity was inhibited only by fetuin, not by mono-, di-, or trisaccharides. This demonstrated the lectin’s selectivity for the N-acetylgalactosamine present on the surface of type A erythrocytes and fetuin. Amaranth lectin exhibited antioxidant activity, which was attributed to the phenolic compounds, amino acids, and specific peptides within the protein structure that are known for their antioxidant properties. Infrared (IR) spectroscopy provided a structural analysis and confirmed lectin glycosylation, a crucial factor in its stability and its ability to bind specific glycans on cell surfaces. Cu2+, Mn2+, and Zn2+ ions were found in the lectin, and these ions were strongly bound to the protein, as dialysis against ethylenediaminetetraacetic acid (EDTA) did not remove them. pH and temperature influenced lectin stability, with higher hemagglutinating activity observed at pH 7, and it remained thermostable at 25 °C.

Details

Title
Purification, Structural Characterization, and Bioactivity of Amaranthus hypochondriacus Lectin
Author
Resendiz-Otero, Maria Fernanda 1 ; Bernardino-Nicanor, Aurea 1   VIAFID ORCID Logo  ; Lugo-Magaña, Olivia 2   VIAFID ORCID Logo  ; Betanzos-Cabrera, Gabriel 3   VIAFID ORCID Logo  ; González-Cruz, Leopoldo 1   VIAFID ORCID Logo  ; Morales-González, José A 4   VIAFID ORCID Logo  ; Acosta-García, Gerardo 5   VIAFID ORCID Logo  ; Fernández-Martínez, Eduardo 6   VIAFID ORCID Logo  ; Salazar-Campos, Arturo 7 ; Valadez-Vega, Carmen 7   VIAFID ORCID Logo 

 Departamento de Ingeniería Bioquímica, Instituto Tecnológico de Celaya, Av. Tecnológico y A. García Cubas S/N, Apartado Postal 57, Celaya 38010, Mexico; [email protected] (M.F.R.-O.); [email protected] (A.B.-N.); [email protected] (L.G.-C.) 
 Preparatoria Número 1, Universidad Autónoma del Estado de Hidalgo, Av. Benito Juárez S/N, Constitución, Pachuca de Soto 42060, Mexico; [email protected] 
 Área Académica de Nutrición, Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Pachuca Hidalgo 42113, Mexico; [email protected] 
 Laboratorio de Medicina de Conservación, Escuela Superior de Medicina, Instituto Politécnico Nacional, México, Plan de San Luis y Díaz Mirón, Col. Casco de Santo Tomás, Del. Miguel Hidalgo, Ciudad de Mexico 11340, Mexico; [email protected] 
 Departamento de Ingeniería Bioquímica y Ambiental, Tecnológico Nacional de México/IT de Celaya, Antonio-García Cubas Pte #600 Esq. Av. Tecnológico, Celaya 38010, Mexico; [email protected] 
 Laboratory of Medicinal Chemistry and Pharmacology, Centro de Investigación en Biología de la Reproducción, Área Académica de Medicina, Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Pachuca Hidalgo 42113, Mexico 
 Área Académica de Medicina, Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Pachuca Hidalgo 42113, Mexico 
First page
5101
Publication year
2024
Publication date
2024
Publisher
MDPI AG
e-ISSN
14203049
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3126014387
Copyright
© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.